Improving beetle karyotype analysis: restriction endonuclease banding of Tenebrio molitor chromosomes

Juan, Carlos; Gosálvez, Jaime; Petitpierre, Eduard

doi:10.1038/hdy.1990.83

Cited by 17 publications

(10 citation statements)

References 31 publications

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“…The Coleoptera constitute the largest animal group with more than 350,000 described species (Lawrence, 1982). The family Tenebrionidae is very conservative in terms of karyological evolution; nearly 60 per cent of the 200 species studied have 2n =20 chromosomes (Juan & Petitpierre, 1990) but some species are known to contain large blocks of heteropycnotic chromatin in meiocytes (Smith, 1952) and variable amounts of C-banding positive chromosomal material (Juan & Petitpierre, 1989). Tenebrio molitor is the only beetle where the satellite DNA has been characterized (Petitpierre et al 1988;Davis & Wyatt, 1989;Ugarkovic et at., 1989).…”

Section: Introductionmentioning

confidence: 99%

Presence of highly repetitive DNA sequences in Tribolium flour-beetles

et al. 1993

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Digestion of genomic DNA from seven species of Tribolium (Coleoptera) with Sau3AI, TaqI and C/al restriction enzymes shows the presence of remarkable amounts of highly repetitive DNA sequences in these species. In Tribolium freemani the sequences are tandemly repeated with a satellite monomer of 166 bp, A-T rich (70.5 per cent), representing 31 per cent of the total genome and located in centromeric chromosome areas as demonstrated by in situ hybridization. The sequence has the potential to form secondary structures such as stems or cruciforms due to the presence of frequent inverted repeats. Tribolium castaneum, T. anaphe and T. madens show homologous sequences to T freemani satellite DNA but T confusum, T audax, T brevicornis and other tenebrionid beetles, such as Tenebrio mo/itor and Misolampus goudoti, do not. A phylogenetic dendrogram, based on the homology and abundance of highly repetitive sequences deduced by dot-blot hybridization, chemotaxonomic and karyological characters, is proposed for the seven studied species of Tribo/ium.

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Section: Introductionmentioning

confidence: 99%

Presence of highly repetitive DNA sequences in Tribolium flour-beetles

et al. 1993

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“…This has been very useful in the analysis of heteromorphisms and/or the analysis of the existence and distribution of different heterochromatic types (Babu & Verma, 1986;Bianchi et at., 1990;Juan et a!., 1990;. We have applied this powerful set of techniques on fixed metaphase chromosomes to determine the heterochromatin differentiation in M.…”

Section: Introductionmentioning

confidence: 99%

Characterization of different chromatin types in Mytilus galloprovincialis L. after C-banding, fluorochrome and restriction endonuclease treatments

1994

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In this study, we have carried out the cytogenetical characterization of Mytilus galloprovincialis L. (2n = 28) using conventional staining and banding techniques such as fluorochromes and restriction endonucleases treatment. Chromosome digestion with trypsin enzyme resulted in a G-banding pattern which allowed us to clearly identify and classify the chromosome pairs of M. galloprovincialis. C-banding and chromomycin A3 staining confirmed the existence of small amounts of constitutive heterochromatin. The treatment of samples with AluI, HaeIII, DpnI, MspI, HpaII and Hinfl restriction endonucleases produced specific banding patterns which demonstrate the potential of endonucleases for chromosome banding in mussels. The results obtained allow us to describe six different types of chromatin in M. galloprovincialis. The type is determined by the response of the chromosomes to the different treatments. Differential digestion by the enzyme pair HpaII-MspI of specific C-band positive heterochromatic areas in some of the chromosomes suggests the presence of methylation.

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“…Smith and Virkki (1978) proposed that 2n=20 with meioformula 9+Xy p is the ancestral karyotype (basal) in the order and that some families are more conserved (Smith and Virkki 1978, Juan et al 1990, Cabral-de-Melo et al 2008). However, several cytogenetic studies show a great variability with respect to both chromosome number and types of sex chromosome systems among different families, in which one can observe a variation from 2n=4 in Chalcolepidius zonatus, Family Elateridae (Ferreira et al 1984) to 2n=69 in Ditomus capito, Family Carabidae (Serrano 1981).…”

Section: Discussionmentioning

confidence: 99%

Evolutionary Dynamics of <i>Tropisternus</i> Karyotype (Coleoptera: Hydrophilidae: Hydrophilini) from Southern Brazil

et al. 2013

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SummaryThe family Hydrophilidae is the largest group of the superfamily Hydrophiloidea to which the largest aquatic Polyphaga are allocated. Phylogenetic studies based on morphological characters suggest that in Hydrophilidae, specifically in Hydrophilini, the body size is related to the group evolution. Despite these morphological analyses, cytogenetic studies of this family are scarce, and there is not a correlation of morphologic and cytogenetic data outlined for this group. In this paper, we analyzed seventeen specimens of Tropisternus mutatus sapucay Fernández & Bachman from rivers from two localities in Southern Brazil. Chromosome analyses showed 2n=18 with a sex chromosome system of the Xy p type (2n=18=16+Xy p ). All the autosomes, including the X p chromosome, were metacentric. The basal number and the possible divergence among the different Hydrophilidae species analyzed to date, as well as the phylogeny proposed for Hydrophilini, are discussed.

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Improving beetle karyotype analysis: restriction endonuclease banding of Tenebrio molitor chromosomes

Cited by 17 publications

References 31 publications

Presence of highly repetitive DNA sequences in Tribolium flour-beetles

Presence of highly repetitive DNA sequences in Tribolium flour-beetles

Characterization of different chromatin types in Mytilus galloprovincialis L. after C-banding, fluorochrome and restriction endonuclease treatments

Evolutionary Dynamics of <i>Tropisternus</i> Karyotype (Coleoptera: Hydrophilidae: Hydrophilini) from Southern Brazil

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