Improving Bioavailability of Hydrophobic Prodrugs through Supramolecular Nanocarriers Based on Recombinant Proteins for Osteosarcoma Treatment

Abstract

Supramolecular nanodrug assembly driven by supramolecular chemistry is becoming a powerful strategy for medication. The potential of engineered proteins as building blocks for nanoformulations is rarely investigated. Here, we developed a new generation of recombinant proteinbased nanodrug carriers, which is very efficient for loading and delivering the hydrophobic prodrug aldoxorubicin. Significantly enhanced anti-tumor effects in osteosarcoma (OS) models were observed. The half-life of the nanodrug reached almost two days and the corresponding bioavailability increased by 17-fold. This is significantly superior to other drug counterparts, empowering long-acting OS treatment scenarios. Importantly, off-target side effects of the prodrug, including cardiotoxicity and lung-metastasis, were greatly mitigated with our medication. Thus, our assembly strategy enables the customized design of advanced nanodelivery systems employing broader biomaterial building blocks for cancer therapy.

Genetically Engineered Polypeptide Adhesive Coacervates for Surgical Applications

“…Meanwhile, a series RESEARCH ARTICLE of surfactants containing 3,4-dihydroxylphenylalanine (DOPA) or azobenzene (Azo) moieties were synthesized (Figure 1B The SUP-surfactant coacervates were prepared by electrostatic complexation of SUPs and the corresponding surfactants. [23,24] Typically, SUPs and surfactants were mixed in an aqueous solution with the initial molar ratio of lysine or glutamic acid to surfactant at 1:1. As a result, the solution became turbid and after centrifugation a viscous SUP-surfactant complex was obtained.…”

Genetically Engineered Polypeptide Adhesive Coacervates for Surgical Applications

“…TheS UP-surfactant coacervates were prepared by electrostatic complexation of SUPs and the corresponding surfactants. [23,24] Ty pically,S UPs and surfactants were mixed in an aqueous solution with the initial molar ratio of lysine or glutamic acid to surfactant at 1:1. As ar esult, the solution became turbid and after centrifugation av iscous SUPsurfactant complex was obtained.…”

Biomacromolecule-based photo-thermal agents for tumor treatment

“…84 In this regard, recombinant proteins or peptides with low immunogenicity have been designed and used for the preparation of photo-responsive hydrogels. 85,86 Recently, Wei and coworkers developed a new type of injectable NIR lightresponsive hydrogel consisting of elastin-like polypeptide (ELP), chitosan, and Au/Ag hybrid nanoparticles (Ag 3 AuS 2 NPs) via electrostatic interactions and forming amide bonds (Fig. 9C).…”