Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding

Jiang, Hui; Yan, Feng; Bao, Liang; X, Li; Gao, Guanjun; Zhang, Qinglu; Xiao, Jinghua; Xu, Caiguo; He, Yuqing

doi:10.1007/s11032-012-9751-6

Cited by 76 publications

(58 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, rice blast resistance has been considered as one of the priorities in the rice breeding program. R-gene linked or specific molecular markers were developed and widely used in the mainstream breeding programs via marker aided selection (MAS) (Jiang et al 2012;Balachiranjeevi et al 2015;Ni et al 2015;Shalini et al 2016;Wu et al 2016;Mi et al 2018). However, many rice blast R-gene haplotypes from different rice lines are almost identical except for a few nucleotide differences, leading to different specificities or even loss of function, e.g, different Pik and Pi2/9 alleles (Zhou et al 2007;Constanzo and Jia 2010).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Ineffective Haplotypes at the Rice Blast Resistance (R) Gene Loci in Chinese Elite Hybrid Rice Varieties Revealed by Sequence-Based Molecular Diagnosis

Xiao

Jiao

Zhu

et al. 2020

Rice

View full text Add to dashboard Cite

Multiple haplotypes at the same rice blast R-gene locus share extremely high sequence similarity, which makes the gene diagnostic method using molecular markers less effective in differentiation from one another. The composition and distribution pattern of deployed R genes/haplotypes in elite rice varieties has not been extensively analyzed. In this study, we employed PCR amplification and sequencing approach for the diagnosis of R-gene haplotypes in 54 Chinese elite rice varieties. A varied number of functional and nonfunctional haplotypes of 4 target major R-gene loci, i.e., Pi2/9, Pi5, Pik, and Pib, were deduced by referring to the reference sequences of known R genes. Functional haplotypes accounted for relatively low frequencies for the Pi2/9 (15%) and Pik (9%) loci but for relatively high frequencies for the Pi5 (50%) and Pib (54%) loci. Intriguingly, significant frequencies of 33%, 39%, 46% of non-functional haplotypes at the Pi2/9, Pik, and Pib loci, respectively, with traceable original donors were identified, suggesting that they were most likely unintentionally spread by using undesirable donors in various breeding programs. In the case of Pi5 locus, only a single haplotype, i.e., Pi5 was identified. The reactions of 54 rice varieties to the differential isolates were evaluated, which showed a good correlation to the frequency of cognate avirulence (Avr) genes or haplotypes in the differential isolates. Four R genes, i.e., Pi2, Piz-t, Pi50, and Pikm were found to contribute significantly to the resistance of the elite rice varieties. Other two genes, Pi9 and Pikh, which were not utilized in rice varieties, showed promising values in breeding durable resistance due to their high resistance frequencies to the contemporary rice blast population. The sequencebased molecular diagnosis provided a promising approach for the identification and verification of haplotypes in different R-gene loci and effective R genes valuable for breeding durable rice resistance to rice blast.

show abstract

Section: Discussionmentioning

confidence: 99%

Prevalence of Ineffective Haplotypes at the Rice Blast Resistance (R) Gene Loci in Chinese Elite Hybrid Rice Varieties Revealed by Sequence-Based Molecular Diagnosis

Xiao

Jiao

Zhu

et al. 2020

Rice

View full text Add to dashboard Cite

show abstract

“…Luo and Yin, (2013) developed a new Thai fragrant rice, 'T5105', which showed a semi-dwarf phenotype with traits such as submergence tolerance and disease resistance to rice blast and bacterial blight. Jiang et al (2012) improved the rice variety Jin32B with blast resistance via the pyramiding of Pi1, Pi2 and D12 genes by marker-assisted selection. Regarding rice eating quality, phenotypes cannot be observed in the field.…”

Section: Discussionmentioning

confidence: 99%

Development of a new fragrant and good eating quality rice variety with stripe virus disease resistance by molecular marker-assisted gene pyramiding

Zhao¹,

Xie²,

Yu³

et al. 2017

Ind. Jrnl. Gen. Plnt. Bree.

View full text Add to dashboard Cite

Rice (Oryza sativa L.) is one of the most important staple crops providing near half of the global population. With the advance of living standards, people demand for novel rice varieties with better quality of eating and fragrance. Unfortunately, the practical application of pesticides to control rice stripe disease results a significant deterioration of rice quality and nutrition. Therefore, it is an urgent need to develop disease resistant rice varieties with good eating quality and fragrance. Here, we present the strategy and practical development of a new rice variety, Shangshida No. 15, by molecular markerassisted gene pyramiding. 'Shangshida No. 15' displayed the aroma trait and the same good eating quality phenotypes as that of Jinfeng rice, a famous rice variety for its good eating quality and high yield in the Yangtze River region of China. Shangshida No. 15 exhibited significantly higher average plant height and 1000-grain weight, while its growth duration was approximately 2 weeks shorter than those of Jinfeng. In conclusion, our study demonstrated a breeding strategy for obtaining high quality rice through molecular marker-assisted gene pyramiding, and the resulting new variety could facilitate further attempts to develop more high eating quality rice varieties together with stripe disease-resistant traits without affecting yields.

show abstract

“…There are three aspects of MABB, namely, positive selection for the target gene using linked markers, negative selection of alleles from the donor parent surrounding the target gene, and background selection for the maximum recovery of the recurrent parent genome using polymorphic markers covering the whole genome1516. MABB has been widely used in rice breeding for disease and insect resistance161718. Likewise, MABB has been used to develop multiple BPH-resistant introgression lines (ILs) and near-isogenic lines (NILs).…”

mentioning

confidence: 99%

Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311

Xiao

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Brown planthopper (BPH) is the most destructive pest of rice in Asia. To date 29 BPH resistance genes have been identified, but only a few genes are being used in breeding due to inefficient markers for marker-assisted selection (MAS) and little knowledge of the real effects of the genes. In this study we individually transferred 13 genes or QTLs (Bph14, QBph3, QBph4, Bph17, Bph15, Bph20, Bph24, Bph6, Bph3, Bph9, Bph10, Bph18 and Bph21) into cultivar 9311 by marker assisted backcross breeding (MABB). Through positive and negative selection we narrowed the segments from donors containing Bph14, Bph15, Bph6 and Bph9 to 100–400 kb. Whole-genome background selection based on a high resolution SNP array was performed to maximize reconstitution of the recurrent parent genome (RPG 99.2–99.9%). All genes reduced BPH growth and development and showed antibiotic responses in seedlings. Based on genetic effects and amino acid sequences of genes in three clusters we inferred that Bph10 and Bph21 might be identical to Bph26, whereas Bph9 and Bph18 were different. Bph15 might be same with Bph17, but QBph4, Bph20 and Bph24 might be different. We believe that these NILs will be useful in rice BPH resistance research and breeding.

show abstract

Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding

Cited by 76 publications

References 20 publications

Prevalence of Ineffective Haplotypes at the Rice Blast Resistance (R) Gene Loci in Chinese Elite Hybrid Rice Varieties Revealed by Sequence-Based Molecular Diagnosis

Prevalence of Ineffective Haplotypes at the Rice Blast Resistance (R) Gene Loci in Chinese Elite Hybrid Rice Varieties Revealed by Sequence-Based Molecular Diagnosis

Development of a new fragrant and good eating quality rice variety with stripe virus disease resistance by molecular marker-assisted gene pyramiding

Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311

Contact Info

Product

Resources

About