Improving cardiac myocytes performance by carbon nanotubes platforms†

Martinelli, Valentina; Cellot, Giada; Fabbro, Alessandra; Bosi, Susanna; Mestroni, Luisa; Ballerini, Laura

doi:10.3389/fphys.2013.00239

Cited by 51 publications

(76 citation statements)

References 39 publications

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“…Traditional 2D gelatin coating culture dishes, prepared as previously described, were used as controls. 4,44,45 Figure 3 shows the live/dead results.…”

Section: Results and Discussionmentioning

confidence: 99%

Biomimetic Polymers for Cardiac Tissue Engineering

et al. 2016

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Heart failure is a morbid disorder characterized by progressive cardiomyocyte (CM) dysfunction and death. Interest in cell-based therapies is growing, but sustainability of injected CMs remains a challenge. To mitigate this, we developed an injectable biomimetic Reverse Thermal Gel (RTG) specifically engineered to support long-term CM survival. This RTG biopolymer provided a solution-based delivery vehicle of CMs, which transitioned to a gel-based matrix shortly after reaching body temperature. In this study we tested the suitability of this biopolymer to sustain CM viability. The RTG was biomolecule-functionalized with poly-l-lysine or laminin. Neonatal rat ventricular myocytes (NRVM) and adult rat ventricular myocytes (ARVM) were cultured in plain-RTG and biomolecule-functionalized-RTG both under 3-dimensional (3D) conditions. Traditional 2D biomolecule-coated dishes were used as controls. We found that the RTG-lysine stimulated NRVM to spread and form heart-like functional syncytia. Regarding cell contraction, in both RTG and RTG-lysine, beating cells were recorded after 21 days. Additionally, more than 50% (p value < 0.05; n = 5) viable ARVMs, characterized by a well-defined cardiac phenotype represented by sarcomeric cross-striations, were found in the RTG-laminin after 8 days. These results exhibit the tremendous potential of a minimally invasive CM transplantation through our designed RTG-cell therapy platform.

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“…Traditional 2D gelatin coating culture dishes, prepared as previously described, were used as controls. 4,44,45 Figure 3 shows the live/dead results.…”

Section: Results and Discussionmentioning

confidence: 99%

Biomimetic Polymers for Cardiac Tissue Engineering

et al. 2016

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“…For example, carbon nanotubes and graphene have been assembled into two-dimensional films (using vacuum filtration and chemical vapor deposition (CVD) methods) and 3D foams (using CVD and sacrificial template-transfer methods) and their cytocompability has been examined for applications in bone, neuron and cardiac tissue engineering [26–38]. However, these methods exhibit several limitations.…”

Section: Introductionmentioning

confidence: 99%

Porous three-dimensional carbon nanotube scaffolds for tissue engineering

Lalwani

Gopalan

D’Agati

et al. 2015

J. Biomed. Mater. Res.

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Assembly of carbon nanomaterials into three-dimensional (3D) architectures is necessary to harness their unique physiochemical properties for tissue engineering and regenerative medicine applications. Herein, we report the fabrication and comprehensive cytocompatibility assessment of 3D chemically crosslinked macro-sized (5–8 mm height and 4–6 mm diameter) porous carbon nanotube (CNT) scaffolds. Scaffolds prepared via radical initiated thermal crosslinking of single- or multi- walled CNTs (SWCNTs and MWCNTs) possess high porosity (>80%), and nano-, micro- and macro-scale interconnected pores. MC3T3 pre-osteoblast cells on MWCNT and SWCNT scaffolds showed good cell viability comparable to poly(lactic-co-glycolic) acid (PLGA) scaffolds after 5 days. Confocal live cell and immunofluorescence imaging showed that MC3T3 cells were metabolically active and could attach, proliferate and infiltrate MWCNT and SWCNT scaffolds. SEM imaging corroborated cell attachment and spreading and suggested that cell morphology is governed by scaffold surface roughness. MC3T3 cells were elongated on scaffolds with high surface roughness (MWCNTs) and rounded on scaffolds with low surface roughness (SWCNTs). The surface roughness of scaffolds may be exploited to control cellular morphology, and in turn govern cell fate. These results indicate that crosslinked MWCNTs and SWCNTs scaffolds are cytocompatible, and open avenues towards development of multifunctional all-carbon scaffolds for tissue engineering applications.

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“…Also, the formation of electrically conductive nanofi bers similar to collagen was observed to improve cell to cell electrical coupling, along with cell adhesion. Despite the proven advantages of carbon nanomaterials in myocardial tissue regeneration, further research needs to be done in potential immune responses due to implantation and possible toxic effects [ 50 ]. Once these concerns are addressed and the safety of these particles is increased, the product has the potential to provide advantages for tissue regeneration that scientists currently do not have.…”

Section: Carbon-based Nanomaterialsmentioning

confidence: 96%