Improving electron trans-inner membrane movements in microbial electrocatalysts

Le, Tao; Xie, Mingshi; Chiew, Geraldine Giap Ying; Wang, Zhijuan; Chen, Wei Ning; Wang, Xin

doi:10.1039/c6cc00976j

Cited by 14 publications

(9 citation statements)

References 49 publications

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“…32 The F/S system also had the highest exchange current I 0 (the intersection point of the Y-axis and the fitted reduction line), indicating that F/S had the highest reduction reaction kinetic activity for H 2 O 2 production. 41,42 The CV curves (Figure 5b) further show the redox enzymes of F/S in the potential range of −0.4−0.0 V, showed much stronger activation than those of S, which may be MtrC (−0.250 V), 43 OmcA (−0.201 V), 44 and flavins (−0.210 V). 19 This indicated that Fe 2 O 3 stimulates Shewanella's ability for dissimilatory reduction.…”

Section: Resultsmentioning

confidence: 91%

Shewanella Drive Fe(III) Reduction to Promote Electro-Fenton Reactions and Enhance Fe Inner-Cycle

Zhao

Cui

et al. 2020

ACS EST Water

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The slow reduction of Fe(III) to Fe(II) in electro-Fenton technology limits pollutant removal and causes Fe sludge production. This study hypothesizes that Shewanella, a dissimilatory iron-reducing bacteria, can accelerate the iron reduction of the Fenton reaction. A Shewanella biofilm coupled with Fe2O3 coated electrode (F/S) was used to drive the electro-Fenton reaction, and compared the results with the singlet Fe2O3 (F) anode and Shewanella biofilm anode (S). Meanwhile, dissolved oxygen (DO) is studied as an important influencing factor. The suitable DO was verified at ∼2 mg/L, phenol removal of F/S was 67% higher than that of the sum of three other singlet systems. The chemical oxygen demand (COD) removal was 72% and 50% higher than that of F and S, respectively. F/S corrosion current (I corr) was 6.3 times higher than F, and induced hematite to transform into Fe2PO5 and FeOOH on the anode. Long-term operation showed that phenol was almost 100% removed for F/S; COD removal was generally 20% higher than F; thus, the toxicity of the effluent could be significantly decreased. The total Fe loss did not exceed 10% at the end of operation. This paper provides a feasibly novel method for electro-Fenton reaction, by employing Fe-reducing bacteria.

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Section: Resultsmentioning

confidence: 91%

Shewanella Drive Fe(III) Reduction to Promote Electro-Fenton Reactions and Enhance Fe Inner-Cycle

Zhao

Cui

et al. 2020

ACS EST Water

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“…The catabolic pathways controlling electron flux exerted significant influence on polymerization rate, which makes them promising targets for future engineering applications. For example, pathways for utilization of different carbon sources (e.g., glucose, glycerol, xylose) or increasing electron-equivalent flux to the inner membrane via NADH dehydrogenase overexpression could be integrated into S. oneidensis to modulate Cu(II) reduction (56)(57)(58). Alternatively, controlling the expression of electron transfer proteins could be used to tune polymerization activity.…”

Section: Discussionmentioning

confidence: 99%

Shewanella oneidensisas a living electrode for controlled radical polymerization

Fan

Dundas

Graham

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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Metabolic engineering has facilitated the production of pharmaceuticals, fuels, and soft materials but is generally limited to optimizing well-defined metabolic pathways. We hypothesized that the reaction space available to metabolic engineering could be expanded by coupling extracellular electron transfer to the performance of an exogenous redox-active metal catalyst. Here we demonstrate that the electroactive bacterium can control the activity of a copper catalyst in atom-transfer radical polymerization (ATRP) via extracellular electron transfer. Using, we achieved precise control over the molecular weight and polydispersity of a bioorthogonal polymer while similar organisms, such as , showed no significant activity. We found that catalyst performance was a strong function of bacterial metabolism and specific electron transport proteins, both of which offer potential biological targets for future applications. Overall, our results suggest that manipulating extracellular electron transport pathways may be a general strategy for incorporating organometallic catalysis into the repertoire of metabolically controlled transformations.

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“…Shewanella oneidensis MR-1 is a γ-proteobacterium that has been used as a pure-culture model organism to study the biochemical underpinnings of microbe-electrode interaction in BESs. Detailed understanding of the Mtr electron transfer pathway in S. oneidensis MR-1 has enabled development of new bioelectrochemical technologies, such as genetically-encoded biosensors 3,4 and strains with increased current production capability 2,5 . Continued development of synthetic biology tools for electrochemically active bacteria, combined with increased knowledge of the molecular basis of extracellular electron transfer can increase functionality of bioelectrochemical technologies 2,6 .…”

Section: Introductionmentioning

confidence: 99%

NADH dehydrogenases Nuo and Nqr1 contribute to extracellular electron transfer by Shewanella oneidensis MR-1 in bioelectrochemical systems

Madsen

TerAvest

2019

Sci Rep

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Shewanella oneidensis MR-1 is quickly becoming a synthetic biology workhorse for bioelectrochemical technologies due to a high level of understanding of its interaction with electrodes. Transmembrane electron transfer via the Mtr pathway has been well characterized, however, the role of NADH dehydrogenases in feeding electrons to Mtr has been only minimally studied in S. oneidensis MR-1. Four NADH dehydrogenases are encoded in the genome, suggesting significant metabolic flexibility in oxidizing NADH under a variety of conditions. A strain lacking the two dehydrogenases essential for aerobic growth exhibited a severe growth defect with an anode (+0.4 VSHE) or Fe(III)-NTA as the terminal electron acceptor. Our study reveals that the same NADH dehydrogenase complexes are utilized under oxic conditions or with a high potential anode. Our study also supports the previously indicated importance of pyruvate dehydrogenase activity in producing NADH during anerobic lactate metabolism. Understanding the role of NADH in extracellular electron transfer may help improve biosensors and give insight into other applications for bioelectrochemical systems.

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Improving electron trans-inner membrane movements in microbial electrocatalysts

Cited by 14 publications

References 49 publications

Shewanella Drive Fe(III) Reduction to Promote Electro-Fenton Reactions and Enhance Fe Inner-Cycle

Shewanella Drive Fe(III) Reduction to Promote Electro-Fenton Reactions and Enhance Fe Inner-Cycle

Shewanella oneidensisas a living electrode for controlled radical polymerization

NADH dehydrogenases Nuo and Nqr1 contribute to extracellular electron transfer by Shewanella oneidensis MR-1 in bioelectrochemical systems

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