Improving Grain Quality: Wheat

Shewry, Peter R.

doi:10.1002/9781118525524.ch9

Seed Genomics 2013

DOI: 10.1002/9781118525524.ch9

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Improving Grain Quality: Wheat

Peter R. Shewry

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2017

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Doubled Haploid Transgenic Wheat Lines by Microspore Transformation

Rustgi

Ankrah

Brew-Appiah

et al. 2017

Methods in Molecular Biology

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Microspores are preferred explant choice for genetic transformation, as their use shortens the duration of obtaining homozygous transformants. All established gene-delivery methods of particle bombardment, electroporation, and cocultivation with Agrobacterium tumefaciens were optimized on androgenic microspores or derived tissues. In the biolistic gene delivery method 35-40 days old haploid microspore embryoids were used for genetic transformation, whereas freshly isolated androgenic microspores were used for genetic transformation in the electroporation and Agrobacterium cocultivation-based methods. The genetic transformation methods of biolistic gene-delivery and electroporation gave rise to the chimeric plants, whereas the method involving cocultivation with Agrobacterium yielded homozygous transformants. These methods were tested on a large number of cultivars belonging to different market classes of wheat, and found to be fairly independent of the explant genotype. Other benefits of using microspores or derived tissues for transformation are: (1) a few explant donors are required to obtain desired transformants and (2) the time required for obtaining homozygous transformants is about 8 months in case of spring wheat genotypes and about a year in case of winter wheat genotypes.

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Doubled Haploid Transgenic Wheat Lines by Microspore Transformation

Rustgi

Ankrah

Brew-Appiah

et al. 2017

Methods in Molecular Biology

View full text Add to dashboard Cite

show abstract

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Improving Grain Quality: Wheat

Cited by 1 publication

References 111 publications

Doubled Haploid Transgenic Wheat Lines by Microspore Transformation

Doubled Haploid Transgenic Wheat Lines by Microspore Transformation

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