Improving hybrid Pennisetum growth and cadmium phytoremediation potential by using Bacillus megaterium BM18-2 spores as biofertilizer

Kamal, Nehal; Liu, Zhiwei; Chen, Qian; Wu, Juan; Zhong, Xiaoyan

doi:10.1016/j.micres.2020.126594

Cited by 32 publications

(9 citation statements)

References 29 publications

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“…It is used in various fields because of its advantages, such as environmentally beneficial, nonpathogenic to humans and animals, small generation time, simple nutritional requirements, and strong stress resistance (Nascimento et al, 2019), especially agriculture. B. megaterium has been extensively studied as the bio-fungicide, biofertilizer, plant growth enhancer, plant growth-promoting rhizobacteria (PGPR) and broad-spectrum biocontrol agent in the agriculture industry (Chakraborty et al, 2006;Boostani et al, 2014;Kamal et al, 2021), which may improve the soil microbial ecosystem and reduce the number of soil-borne plant pathogens. In addition, isolated active antibacterial substances could be applied onto the soil to improve the growth of plants and reduce the number of plant pathogens (Ryan et al, 2009;Hu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Antibacterial Bioactive Compounds From Bacillus megaterium L2

Xie

Peng

et al. 2021

Front. Microbiol.

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Bacterial metabolites exhibit a variety of biologically active compounds including antibacterial and antifungal activities. It is well known that Bacillus is considered to be a promising source of bioactive secondary metabolites. Most plant pathogens have an incredible ability to mutate and acquire resistance, causing major economic losses in the agricultural field. Therefore, it is necessary to use the natural antibacterial compounds in microbes to control plant pathogens. This study was conducted to investigate the bio-active compounds of Bacillus megaterium L2. According to the activity guidance of Agrobacterium tumefaciens T-37, Erwinia carotovora EC-1 and Ralstonia solanacearum RS-2, five monomeric compounds, including erucamide (1), behenic acid (2), palmitic acid (3), phenylacetic acid (4), and β-sitosterol (5), were fractionated and purified from the crude ethyl acetate extract of B. megaterium. To our knowledge, all compounds were isolated from the bacterium for the first time. To understand the antimicrobial activity of these compounds, and their minimum inhibitory concentrations (MICs) (range: 0.98∼500 μg/mL) were determined by the broth microdilution method. For the three tested pathogens, palmitic acid exhibited almost no antibacterial activity (>500 μg/mL), while erucamide had moderate antibacterial activity (MIC = 500 μg/mL). Behenic acid showed MICs of 250 μg/mL against T-37 and RS-2 strains with an antibacterial activity. β-sitosterol showed significant antimicrobial activity against RS-2. β-sitosterol showed remarkable antimicrobial activity against RS-2 with an MIC of 15.6 μg/mL. In addition, with the antimicrobial activity, against T-37 (62.5 μg/mL) and against EC-1 (125 μg/mL) and RS-2 (15.6 μg/mL) strains notably, phenylacetic acid may be interesting for the prevention and control of phytopathogenic bacteria. Our findings suggest that isolated compounds such as behenic acid, β-sitosterol, and phenylacetic acid may be promising candidates for natural antimicrobial agents.

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Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Antibacterial Bioactive Compounds From Bacillus megaterium L2

Xie

Peng

et al. 2021

Front. Microbiol.

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“…Microbes are pivotal players in the bioremediation of polluted soil, with certain functional microbes capable of reducing soil contamination levels [48]. Several growth parameters of the plant H. Pennisetum, including shoot length, biomass, and dry weight, indicate that inoculation with Bacillus significantly improves plant growth in the soil [49]. Compared to the CK treatment, the application of Bacillus cereus increased the diversity of bacterial communities in the non-rhizospheric soil of the lawn plants but reduced the richness of bacterial communities in the rhizospheric soil (Table 2).…”

Section: The Response Relationship Between Plant Remediation and Soil...mentioning

confidence: 99%

Microbiological Mechanisms of Collaborative Remediation of Cadmium-Contaminated Soil with Bacillus cereus and Lawn Plants

Zhou,

Yang,

Chen

et al. 2024

Plants

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Severe cadmium contamination poses a serious threat to food security and human health. Plant–microbial combined remediation represents a potential technique for reducing heavy metals in soil. The main objective of this study is to explore the remediation mechanism of cadmium-contaminated soil using a combined approach of lawn plants and microbes. The target bacterium Bacillus cereus was selected from cadmium-contaminated soil in mining areas, and two lawn plants (Festuca arundinacea A‘rid III’ and Poa pratensis M‘idnight II’) were chosen as the target plants. We investigated the remediation effect of different concentrations of bacterial solution on cadmium-contaminated soil using two lawn plants through pot experiments, as well as the impact on the soil microbial community structure. The results demonstrate that Bacillus cereus promotes plant growth, and the combined action of lawn plants and Bacillus cereus improves soil quality, enhancing the bioavailability of cadmium in the soil. At a bacterial suspension concentration of 105 CFU/mL, the optimal remediation treatment was observed. The removal efficiency of cadmium in the soil under Festuca arundinacea and Poa pratensis treatments reached 33.69% and 33.33%, respectively. Additionally, the content of bioavailable cadmium in the rhizosphere soil increased by up to 13.43% and 26.54%, respectively. Bacillus cereus increased the bacterial diversity in the non-rhizosphere soil of both lawn plants but reduced it in the rhizosphere soil. Additionally, the relative abundance of Actinobacteriota and Firmicutes, which have potential for heavy metal remediation, increased after the application of the bacterial solution. This study demonstrates that Bacillus cereus can enhance the potential of lawn plants to remediate cadmium-contaminated soil and reshape the microbial communities in both rhizosphere and non-rhizosphere soils.

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“…The isolates were identified morphologically and biochemically using Bergey's Manual of Systematic Bacteriology. The isolates were also characterized physiologically and biochemically using a Colorimetric reagent card from a VITEK 2 analyzer (BioMerieux, France) (Kamal et al,2021).…”

Section: Characterization and Identification Of Isolatesmentioning

confidence: 99%

Study of antibacterial activity and cytotoxicity of the bioactive compound of Bacillus megaterium L2 strains isolated from the oral cavity of hospital workers and visitors at Dental Health Centre, Babylon, Iraq

Jassim

Al-Yassiry

Awadh

2023

JANS

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Because of the resistance of pathogenic bacteria to antibiotics, there is an urgent necessity to search for new antibiotics produced by Bacillus spp., which are characterized by their capability to produce secondary metabolites with high efficacy against numerous types of pathogenic bacteria. A total of 40 Bacillus isolates were isolated from the mouths of 150 volunteers from the Dental Health Center in Babylon and diagnosed based on phenotypic characteristics and biochemical and physiological reaction tests with a colorimetric reagent card using the VITEK2 analyzer. The active compounds were extracted from Bacillus megaterium L2 and their antibacterial activity was tested against a group of gram-negative and gram-positive bacteria. The Minimum inhibitory concentration (MIC) of the extract was estimated, whereas 16 isolates showed high effectiveness against pathogenic bacteria, with the zone of inhibition ranging from 8-22 mm and the MIC ranging from 0.25–6.25 mg/ml. The active compounds were extracted, purified, and detected by Thin-layer chromatography (TLC), Infrared (IR) spectroscopy, and Ultraviolet (UV) spectroscopy. The cytotoxic activity of the extracts was studied using the MCF7 cell line. This showed that cytotoxicity effects on valid object count, nuclear morphology, and total nuclear intensity ranged from 17.245-441.24 and the cytotoxic effect on cell membrane permeability, mitochondrial membrane potential, and cytochrome C ranged from 49.04-601.79 Among the isolates, Bacillus megaterium L2(B9) was the best isolated strain of bacteria that was the most effective against anti-pathogenic bacterial strains- Gram positive (Staphylococcus pyogenes NCTC 8198 and St. aureus ATCC 29213) and gram negative (Pseudomonas aeruginosa RW109, Escherichia coli O157, and Salmonella typhi Ty2) and was non-toxic to human cells (MCF7).

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Improving hybrid Pennisetum growth and cadmium phytoremediation potential by using Bacillus megaterium BM18-2 spores as biofertilizer

Cited by 32 publications

References 29 publications

Isolation and Identification of Antibacterial Bioactive Compounds From Bacillus megaterium L2

Isolation and Identification of Antibacterial Bioactive Compounds From Bacillus megaterium L2

Microbiological Mechanisms of Collaborative Remediation of Cadmium-Contaminated Soil with Bacillus cereus and Lawn Plants

Study of antibacterial activity and cytotoxicity of the bioactive compound of Bacillus megaterium L2 strains isolated from the oral cavity of hospital workers and visitors at Dental Health Centre, Babylon, Iraq

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