Improving Nitrogen Use Efficient in Crop Plants Using Biotechnology Approaches

Beatty, Perrin H.; Good, Allen G.

doi:10.1007/978-3-319-92958-3_2

Cited by 11 publications

(14 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conversely, the photosynthetic activity in N-inefficient genotypes especially XLZ-30 decreased, which might have been due to inhibition in the photosystem, as many genes involved in the photosystem were downregulated under contrasting nitrogen supply [46]. Similarly, low nitrogen significantly decreased the photosynthetic activities in cotton genotypes, which are consistent with the previous results in which Arabidopsis, rice, maize, wheat, and other nitrogen-deficient plants were reduced [47][48][49][50]. This reduction in photosynthetic activities under low nitrogen may also be due to poor carboxylation, as noted from the high levels of intercellular CO 2 concentration, which are in line with the results obtained in rice and sunflower [51].…”

Section: Variations In Morphophysiological and Biochemical Traits Amosupporting

confidence: 89%

Variations in Nitrogen Metabolism are Closely Linked with Nitrogen Uptake and Utilization Efficiency in Cotton Genotypes under Various Nitrogen Supplies

Iqbal

Dong

Wang

et al. 2020

Plants

View full text Add to dashboard Cite

Cotton production is highly sensitive to nitrogen (N) fertilization, whose excessive use is responsible for human and environmental problems. Lowering N supply together with the selection of N-efficient genotypes, more able to uptake, utilize, and remobilize the available N, could be a challenge to maintain high cotton production sustainably. The current study aimed to explore the intraspecific variation among four cotton genotypes in response to various N supplies, in order to identify the most distinct N-efficient genotypes and their nitrogen use efficiency (NUE)-related traits in hydroponic culture. On the basis of shoot dry matter, CCRI-69 and XLZ-30 were identified as N-efficient and N-inefficient genotypes, respectively, and these results were confirmed by their contrasting N metabolism, uptake (NUpE), and utilization efficiency (NUtE). Overall, our results indicated the key role of shoot glutamine synthetase (GS) and root total soluble protein in NUtE. Conversely, tissue N concentration and N-metabolizing enzymes were considered as the key traits in conferring high NUpE. The remobilization of N from the shoot to roots by high shoot GS activity may be a strategy to enhance root total soluble protein, which improves root growth for N uptake and NUE. In future, multi-omics studies will be employed to focus on the key genes and pathways involved in N metabolism and their role in improving NUE.

show abstract

Section: Variations In Morphophysiological and Biochemical Traits Amosupporting

confidence: 89%

Variations in Nitrogen Metabolism are Closely Linked with Nitrogen Uptake and Utilization Efficiency in Cotton Genotypes under Various Nitrogen Supplies

Iqbal

Dong

Wang

et al. 2020

Plants

View full text Add to dashboard Cite

show abstract

“…Large quantities of nitrogenous fertilizers are required to attain maximal yields in cereals such as maize, wheat, and rice, which account for 70% of the worldwide food production [1,2]. In consideration of both environmental and economic concerns, several studies were thus conducted to improve nitrogen use efficiency (NUE) in these crops [3,4]. The efficiency of N use is generally defined as the yield obtained per unit of available N in the soil (supplied by the soil + N fertilizer).…”

Section: Introductionmentioning

confidence: 99%

“…The efficiency of N use is generally defined as the yield obtained per unit of available N in the soil (supplied by the soil + N fertilizer). It is the product of absorption efficiency (amount of absorbed N/quantity of available N) and use efficiency (yield/absorbed N), [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, studies in which physiological and molecular genetic approaches were combined have been undertaken in order to identify the limiting steps involved in the control of N uptake, assimilation and recycling in many crops [3,4] including maize [6]. Different approaches, including whole-plant physiological [7] and quantitative genetic studies [8] were originally developed to identify both structural and regulatory candidate genes, as well as the key enzymatic reactions involved in the control of NUE.…”

Section: Introductionmentioning

confidence: 99%

“…To study the role of the different enzymes involved in providing C to the ammonium assimilation pathway and in the subsequent synthesis of amino acids, a large number of reverse and forward genetic approaches have been conducted over the past two decades both in model and crop species [4,21]. Both glutamine and glutamate are two key N metabolites that act as amino group donors to form other amino acids used for transport, structural and storage protein synthesis and the synthesis of nucleotides for the formation of RNA and DNA [11,27].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

NADH-GOGAT Overexpression Does Not Improve Maize (Zea mays L.) Performance Even When Pyramiding with NAD-IDH, GDH and GS

Cañas

Yesbergenova-Cuny

Belanger

et al. 2020

Plants

View full text Add to dashboard Cite

Maize plants overexpressing NADH-GOGAT were produced in order to determine if boosting 2-Oxoglurate production used as a carbon skeleton for the biosynthesis of amino acids will improve plant biomass and kernel production. The NADH-GOGAT enzyme recycles glutamate and incorporates carbon skeletons into the ammonium assimilation pathway using the organic acid 2-Oxoglutarate as a substrate. Gene pyramiding was then conducted with NAD-IDH and NADH-GDH, two enzymes also involved in the synthesis of 2-Oxoglurate. NADH-GOGAT overexpression was detrimental for shoot biomass production but did not markedly affect kernel yield. Additional NAD-IDH and NADH-GDH activity did not improve plant performance. A decrease in kernel production was observed when NADH-GDH was pyramided to NADH-GOGAT and NAD-IDH. This decrease could not be restored even when additional cytosolic GS activity was present in the plants overexpressing the three enzymes producing 2-Oxoglutarate. Detailed leaf metabolic profiling of the different transgenic plants revealed that the NADH-GOGAT over-expressors were characterized by an accumulation of amino acids derived from glutamate and a decrease in the amount of carbohydrates further used to provide carbon skeletons for its synthesis. The study suggests that 2-Oxoglutarate synthesis is a key element acting at the interface of carbohydrate and amino acid metabolism and that its accumulation induces an imbalance of primary carbon and nitrogen metabolism that is detrimental for maize productivity.

show abstract

Cocoa Clones Reveal Variation in Plant Biomass, Root Nitrogen Uptake, and Apparent Nitrogen Recovery at the Seedling Stage

Ruseani

Vanhove

Susilo

et al. 2022

J Soil Sci Plant Nutr

View full text Add to dashboard Cite

We aimed to investigate variation in growth, biomass, macro-nutrient uptake, and nitrogen (N) use efficiency indices of cocoa (Theobroma cocoa L.) scion clones under two different nitrogen conditions. Ten clones were top-grafted onto 4-month-old rootstocks clone MCC 02. Each seedling received either 0 or 4.6 g of nitrogen applied as urea fertilizer. The design was arranged as a two-factor experiment with four replicates in a factorial randomized block design. We measured stem diameter, biomass, nutrient concentration, and calculated nutrient uptake, nitrogen use efficiency (NUE), agronomic efficiency (AE), apparent nitrogen recovery (ANR), and physiological efficiency (PE). We found that clones significantly affected stem diameter and shoot biomass of both rootstocks and scions. Clones differed in root magnesium (Mg) and shoot calcium (Ca) concentration, root N, and shoot Mg uptake. Furthermore, a significant clone effect was observed on ANR, with local clones showing higher ANR than introduced clones. Nitrogen application significantly improved stem diameter and biomass. Compared to unfertilized plants, a significantly higher N concentration was observed in roots, resulting in higher N uptake in both roots and shoots. Nitrogen application increased shoot Ca concentration and uptake but decreased shoot phosphorus (P) and Mg concentrations. Our results suggest genotypic variability in nitrogen uptake efficiency in clonal cocoa. Clones with high nitrogen uptake efficiency are valuable genetic resources for breeding cocoa clones that efficiently use available N and, therefore, grow better under N-deficient conditions.

show abstract

Improving Nitrogen Use Efficient in Crop Plants Using Biotechnology Approaches

Cited by 11 publications

References 97 publications

Variations in Nitrogen Metabolism are Closely Linked with Nitrogen Uptake and Utilization Efficiency in Cotton Genotypes under Various Nitrogen Supplies

Variations in Nitrogen Metabolism are Closely Linked with Nitrogen Uptake and Utilization Efficiency in Cotton Genotypes under Various Nitrogen Supplies

NADH-GOGAT Overexpression Does Not Improve Maize (Zea mays L.) Performance Even When Pyramiding with NAD-IDH, GDH and GS

Cocoa Clones Reveal Variation in Plant Biomass, Root Nitrogen Uptake, and Apparent Nitrogen Recovery at the Seedling Stage

Contact Info

Product

Resources

About