Improving osteoblast functions and bone formation upon BMP-2 immobilization on titanium modified with heparin

Thermo-responsive poly(N-isopropylacrylamide)-grafted hollow fiber membranes for osteoblasts culture and non-invasive harvest

“…9(A), after being cultured for 4 and 8 days, the ALP activity of osteoblasts grown on thermo-responsive HFMs was not significantly different from that on unmodified HFMs. It was reported that ALP was widely used as markers for early differentiation of osteoblasts [39]. Therefore, this observation indicated that the grafted PNIPAAm had no obvious influence on the differentiation of osteoblasts at early stage.…”

Study of a new bone-targeting titanium implant–bone interface

“…[5][6][7] Different strategies have been proposed to improve implant-bone interfaces on mainstream titanium (Ti) and Ti alloy implants, including the manipulation of surface topography, application of biomimetic coatings, and local delivery of drugs to enhance bone formation or inhibit bone resorption. [8][9][10] To enhance the osseointegration of Ti implants in either normal or osteoporotic subjects, in some ways, it is important to induce and enhance the osteoblast functions more precisely and more effectively on the implant-bone interface. Thus, the studies of bone-targeting Ti implant-bone interface with bone-targeting effect 6308 liu et al have become a focus recently.…”

Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering

“…Moreover, the amount of calcium deposited on MG-63 cells grown on BMP-2/Hep-PMS was significantly greater than cells grown on BMP-2/PMS. The results of Kim et al [19,42,43] demonstrated that periodontal ligament cells (PDLCs) or MG-63 cells grown on BMP-2-immobilized PCL fiber or Ti with heparin showed significant differences in ALP activity and calcium deposition compared to PCL fibers or Ti alone. As we have briefly mentioned in our results and previous studies, BMP-2 directly affects the early and late differentiation of MG-63 cells.…”

“…It is well known that heparin is composed of many sulfate and carboxyl groups and has good binding affinities for BMP-2. Huh et al [41] and Kim et al [42] showed that BMP-2 from Ti and bovine bone (Bio-Oss) modified with heparin were released in a sustained fashion compared to BMP-2 from Ti and bovine bone (Bio-Oss). Consistent with the previous studies, this study showed the sustained release of BMP-2 from BMP-2/Hep-PMS.…”

“…ALP activity is typically used to confirm early differentiation of osteoblast-like cells [9,19,34,42]. The ALP activity of MG-63 cells grown on PMS, BMP-2/PMS, and BMP-2/Hep-PMS were evaluated for culture periods of up to 10 days.…”