Thermo-responsive poly(N-isopropylacrylamide)-grafted hollow fiber membranes for osteoblasts culture and non-invasive harvest

Zhuang, Meiling; Liu, Tianqing; Song, Kedong; Li, Xiangqin

doi:10.1016/j.msec.2015.05.040

Cited by 14 publications

(8 citation statements)

References 44 publications

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“…After polymerization, notable IR peaks including amide I (1650 cm −1 ), amide II (1547 cm −1 ), NH stretching vibration (3300 cm −1 ), and CH 3 group (3025 cm −1 ) are evident. Similar results have been reported by other researchers, when they grafted PNIPAAm on the surface of TCPS substrates . It should be noted, CO peak partly interferes with NH bending band, resulting a twin peak around 1620–1640 cm −1 .…”

Section: Discussionsupporting

confidence: 90%

Switchable phase transition behavior of thermoresponsive substrates for cell sheet engineering

Mokhtarinia

Nourbakhsh

Masaeli

et al. 2018

J Polym Sci B Polym Phys

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Recently, there are significant interests in the development of biomaterials with nonlinear response to an external stimulus. Thermoresponsive polymers as a well‐known class of stimuli‐responsive materials represent reversible hydrophilicity/hydrophobicity characteristics around a critical temperature. This switchable behavior applies for nondestructive cellular detachment from cultivation substrates. In this study, poly (N‐isopropylacrylamide) (PNIPAAm)‐grafted dishes were made up to harvest retinal pigmented epithelial (RPE) and periodontal ligament cell (PDLC) sheets. Wettability assessments verified that all functionalized surfaces were inverted from hydrophilic to hydrophobic state when the temperature rises from lower critical solution temperature (LCST) at 37 °C. Other physicochemical characteristics such as chemical composition, grafting thickness, and surface topography were investigated through attenuated total reflection Fourier transform infrared spectroscopy (ATR‐FTIR) and atomic force microscopy (AFM). ATR‐FTIR results showed typical peaks of amide group corresponding to successful PNIPAAm polymerization. AFM microscopy results also proved creating a rough PNIPAAm layer with thickness of 29.2 nm after grafting process in the mixture of methanol and water. Cell culture experiments showed an irreversible cellular attachment/detachment from modified surfaces upon temperature changes. These results introduced thermoresponsive TCPS to noninvasively harvest RPE and PDLCs sheets especially for application in scaffold‐free tissue engineering decorations. © 2018 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2018, 56, 1567–1576

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Section: Discussionsupporting

confidence: 90%

Switchable phase transition behavior of thermoresponsive substrates for cell sheet engineering

Mokhtarinia

Nourbakhsh

Masaeli

et al. 2018

J Polym Sci B Polym Phys

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“…Steam sterilization was already proven to be suitable for PNIPAM microgels, which can also be concluded for PVCL microgels according to the DLS data. [ 36 ] Also, steam sterilization is a common, feasible, and widely used technique in bioprocess engineering and is therefore chosen to be the standard treatment for the present work. Hence, hollow fiber membranes were coated with PVCL microgels, integrated into a custom made bioreactor and steam sterilized at 121 °C.…”

Section: Resultsmentioning

confidence: 99%

Trypsin‐Free Cultivation of 3D Mini‐Tissues in an Adaptive Membrane Bioreactor

et al. 2020

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The production of large scaffold‐free tissues is a key challenge in regenerative medicine. Nowadays, temperature‐responsive polymers allow intact tissue harvesting without needing proteolytic enzymes. This method is limited to tissue culture plastic with limited upscaling capacity and plain process control. Here, a thermoresponsive hollow fiber membrane bioreactor is presented to produce large scaffold‐free tissues. Intact tissues, rich in cell‐to‐cell connections and ECM, are harvested from a poly(N‐vinylcaprolactam) microgel functionalized poly(ether sulfone)/poly(vinylpyrrolidone) hollow fiber membrane by a temperature shift. The harvested 3D tissues adhere in successive cultivation and exhibit high vitality for several days. The facile adsorptive coating waives the need for extensive surface treatment. The research is anticipated to be a starting point for upscaling the production of interconnected tissues enabling new opportunities in regenerative medicine, large‐scale drug screening on physiological relevant tissues, and potentially opening new chances in cell‐based therapies.

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“…The new materials produced by Zhuang may solve this problem and promote the better application of hollow fiber bioreactors. 77 3.3. Other Technologies for Better Control of Microenvironment.…”

Section: Acs Applied Bio Materialsmentioning

confidence: 99%

“…However, it is still a difficult problem for the harvest of cells in hollow fibers and some researchers have done some work on it. The new materials produced by Zhuang may solve this problem and promote the better application of hollow fiber bioreactors …”

Section: Smart-bioreactor For Stem Cell Proliferation and Differentia...mentioning

confidence: 99%

Bioreactor Synergy with 3D Scaffolds: New Era for Stem Cells Culture

Huang

Liu

et al. 2018

ACS Appl. Bio Mater.

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Because of unparalleled advantages over other cells, stem cells are widely used in genetic diagnosis, drug delivery, and regenerative medicine. However, because the content of stem cells in the organism is far from satisfactory, it is of great significance of stem cells to in vitro proliferation and differentiation. However, many stem cell cultures have low expansion efficiency and stem cells lose their value-adding ability and differentiation ability after many generations of culture. To solve these problems, people hope to more realistically simulate the microenvironment in which stem cells grow in vivo. Cell scaffolds gradually evolve from 2D structures to 3D structures. The addition of growth factors influences cell behavior from internal biochemical conditions and the development of smart bioreactors gradually make progress to more precise regulate the external conditions of stem cell. In this paper, the key factors for constructing the microenvironment of stem cell growth were analyzed, and we reviewed the application of bioreactors and 3D scaffolds in stem cell cultivation. Finally, this paper indicated the development directions of stem cell culture in vitro.

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Thermo-responsive poly(N-isopropylacrylamide)-grafted hollow fiber membranes for osteoblasts culture and non-invasive harvest

Cited by 14 publications

References 44 publications

Switchable phase transition behavior of thermoresponsive substrates for cell sheet engineering

Switchable phase transition behavior of thermoresponsive substrates for cell sheet engineering

Trypsin‐Free Cultivation of 3D Mini‐Tissues in an Adaptive Membrane Bioreactor

Bioreactor Synergy with 3D Scaffolds: New Era for Stem Cells Culture

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