Abstract. Fat, cholesterol and fatty acid profile of breast and thigh meat of 12 Muscovy ducks (6 drakes) aged 8 weeks from the local farm in Bogor, West Java were analyzed in thus study. In general, Muscovy ducks were fed with rice bran twice a day and free-ranged in the backyard. The nutrition of rice bran is varied in crude protein, crude fiber, fat, and ash content. Muscovy ducks were slaughtered and the percentage of carcass weight, fat, cholesterol and fatty acid profile of the breast and thigh meat were measured and analyzed. The result showed that the breast meat had higher (P<0.05) ω3 total fatty acid EPA (C20:5ω3) but DHA (C22:6ω3) and linolenic acid (C18:3ω3) were not significant compared to that of thigh meat. Concentration of linolenic acid in breast meat ranged from 0.26 to 0.51% of fat and 0.35 to 0.39% of fat in thigh meat. Linoleic acid (C18:2ω6) in breast and thigh meat was 7.64 -10.73% and 5.17 -13.93 % fat, respectively. DHA concentration ranged from 0.13-1.31% fat in breast meat and EPA in thigh meat was approximately 0.08-0.32 % fat. EPA was significantly higher (P<0.05) in breast meat (0.03-0.28 %) than thigh meat (0.05-0.08 %) The study produced ω 6:3 ratio ranged 6.50-24.14 and 8.94-31.64 in breast and thigh meat, respectively. Key words: Muscovy duck, fatty acid, fat, cholesterol.Abstrak. Penelitian ini bertujuan untuk mendapatkan informasi tentang kandungan leamk,cholesterol dan profil asam lemak dari daging dada dan daging paha entok (Muscovy duck) yang dipelihara secara tradisional oleh masyarakat. Entok diberi pakan dedak padi yang mempunyai kualitas rendah dan beragam dari 6 peternak yang ada di kota Bogor, Jawa Barat, Indonesia. Dedak padi diberikan dua kali sehari yaitu pagi dan siang kemudian entok di lepas disekitar halaman rumah. Penelitian ini menggunakan 12 sampel entok berumur 8 minggu kemudian dipotong dan diukur karkas, persentase daging dada dan paha dan kandungan lemak,cholesterol dan asam lemak. Hasil penelitian diperoleh daging dada lebih tinggi kandungan total asam lemak ω 3 (P<0.05), EPA (C20:5ω3) (P<0.05) daripada daging paha tetapi DHA (C22:6ω 3) tidak berbeda nyata. Kadar asam linolenat (C18: 3ω3) pada daging dada berkisar dari 0,26 to 0,51% total lemak dan 0,35 to 0,39 % total lemak di daging paha. Asam linoleat (C18:2 ω 6) berkisar 7,64 -10,73% total lemak di daging dada dan 5,17 -13,93 % total lemak di daging paha. Kadar DHA (C22:6ω 3) berkisar 0,13-1,31% total lemak di daging dada dan EPA berkisar 0,08-0,32 % total lemak di daging paha. Kadar EPA lebih tinggi (P<0,05) di daging dada dibandingkan daging paha berkisar 0,03-0,28 % total lemak (daging dada) sampai 0,05-0,08 % total lemak (daging paha). Penelitian ini menghasilkan rasio ω 6/ ω 3 berkisar 6,50-24,14 di daging dada dan 8,94-31,64 di daging paha.