Improving production of hyperthermostable and high maltose-forming α-amylase by an extreme thermophile Geobacillus thermoleovorans using response surface methodology and its applications

Rao, J.L. Uma Maheswar; Satyanarayana, T.

doi:10.1016/j.biortech.2005.12.022

Cited by 66 publications

(29 citation statements)

References 27 publications

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“…Glucoamylase production predicted by the statistical design was very close to the experimental enzyme titers, which reflected the accuracy of the model [24]. Response surface optimization has been successfully used earlier for optimizing fermentation variables for the production of α-amylase [15], phytase [25], and chitinase [26].…”

Section: Discussionmentioning

confidence: 98%

“…Design Expert version 6.0 provides rotatable 3-D plots of response surfaces, which help in establishing the relationship between one or more measured responses and vital input factors [11]. With the development of computing software and their wide applications, RSM has emerged as a valuable technique in many areas of biotechnology for optimizing the process parameters [14,15].…”

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confidence: 99%

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Production of Raw Starch-Saccharifying Thermostable and Neutral Glucoamylase by the Thermophilic Mold Thermomucor indicae-seudaticae in Submerged Fermentation

Kumar

Satyanarayana

2007

Appl Biochem Biotechnol

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The effect of temperature on the performance of an anaerobic sequencing biofilm batch reactor (ASBBR) with liquid-phase recirculation was assessed. Assays were performed using a recirculation velocity of 0.20 cm/s, 8-h cycles, and an average treated synthetic wastewater volume of 2 L/cycle with a concentration of 500 mg of Chemical Oxygen Demand (COD)/L. Operation temperatures were 15, 20, 25, 30, and 35 degrees C. At 25, 30, and 35 degrees C, organic matter removal efficiencies for filtered samples ranged from 81 to 83%. At lower temperatures, namely 15 and 20 degrees C, removal efficiency decreased significantly to 61 and 65%, respectively. A first-order model could be fitted to the experimental concentration profile values. The first-order kinetic parameter value of this model varied from 0.46 to 0.81 h-1 considering the lowest and highest temperature studied. Moreover, analysis of the removal profile values allowed fitting of an Arrhenius-type equation with an activation energy of 5715 cal/mol.

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Section: Discussionmentioning

confidence: 98%

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confidence: 99%

Production of Raw Starch-Saccharifying Thermostable and Neutral Glucoamylase by the Thermophilic Mold Thermomucor indicae-seudaticae in Submerged Fermentation

Kumar

Satyanarayana

2007

Appl Biochem Biotechnol

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“…The Aspergillus species produce a large variety of extracellular enzymes of which amylases are of world-wide interest in fermentation, food, pharmaceutical, textile and paper industries (Bhargav et al 2008;Uma Maheswar Rao and Satyanarayana 2007). Production of amylases by Aspergillus strains in both submerged liquid fermentation (SLF) and solid-state fermentation (SSF) by using different food wastes or agricultural residues has been thoroughly studied (Ellaiah et al 2002;Francis et al 2002Francis et al , 2003Murado et al 1997;Salas et al 2006).…”

Section: Introductionmentioning

confidence: 98%

Optimization of amylase production by Aspergillus niger in solid-state fermentation using sugarcane bagasse as solid support material

Rosés

Guerra

2009

World J Microbiol Biotechnol

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Synthesis of amylase by Aspergillus niger strain UO-01 under solid-state fermentation with sugarcane bagasse was optimized by using response surface methodology and empirical modelling. The process parameters tested were particle size of sugarcane bagasse, incubation temperature and pH, moisture level of solid support material and the concentrations of inoculum, total sugars, nitrogen and phosphorous. The optimum conditions for high amylase production (457.82 EU/g of dry support) were particle size of bagasse in the range of 6-8 mm, incubation temperature and pH: 30.2°C and 6.0, moisture content of bagasse: 75.3%, inoculum concentration: 1 9 10 7 spores/g of dry support and concentrations of starch, yeast extract and KH 2 PO 4 : 70.5, 11.59 and 9.83 mg/ g of dry support, respectively. After optimization, enzyme production was assayed at the optimized conditions. The results obtained corroborate the effectiveness and reliability of the empirical models obtained.

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“…Fungal and bacterial amylases are widely used for commercial application in food processing industries (Burhan et al, 2003). The Aspergillus species produce a variety of extracellular enzymes of which amylases are of the world-wide interest in fermentation, food, pharmaceutical, textile and paper industries (Rao and Satyanarayana, 2007;Bhargav et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Amylase Production from Solid State Fermentation and Submerged Liquid Fermentation by <i>Aspergillus niger</i>

Dharani¹,

Kumaran

2012

Bangladesh J. Sci. Ind. Res.

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The purpose of this work is to study the optimized cultural conditions for the production of amylase by Aspergillus niger in solid state and submerged liquid fermentation. Four solid substrates banana peel, corn, potato and tapioca with different moisture conditions were taken for solid state fermentation (SSF). Basal medium was used for submerged liquid fermentation (SLF) with different pH (3 to 8), temperature (25, 30, 35 and 40ºC), carbon concentration (1, 2 and 3 g) and nitrogen source (0.1, 0.2 and 0.3 g). In SSF, tapioca yielded highest amylase activity and specific activity (4.43U/ml and 4.58U/mg) at 50% moisture content. In SLF, 2 g starch and 0.3 g peptone concentration showed 0.78 and 1.23 U/ml amylase activities under the optimum pH (5) and temperature (30ºC) the amylase activities reached to 0.86 U/ml and 0.76 U/ml respectively. In SSF using tapioca as substrate the enzyme yield is about five times higher than that achieved with submerged liquid culture.

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Improving production of hyperthermostable and high maltose-forming α-amylase by an extreme thermophile Geobacillus thermoleovorans using response surface methodology and its applications

Cited by 66 publications

References 27 publications

Production of Raw Starch-Saccharifying Thermostable and Neutral Glucoamylase by the Thermophilic Mold Thermomucor indicae-seudaticae in Submerged Fermentation

Production of Raw Starch-Saccharifying Thermostable and Neutral Glucoamylase by the Thermophilic Mold Thermomucor indicae-seudaticae in Submerged Fermentation

Optimization of amylase production by Aspergillus niger in solid-state fermentation using sugarcane bagasse as solid support material

Amylase Production from Solid State Fermentation and Submerged Liquid Fermentation by <i>Aspergillus niger</i>

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