1-Methylcyclopropene (1-MCP), an ethylene action inhibitor, is routinely applied to fruit as a postharvest treatment prior to cold storage to extend fruit storability and posterior shelf life. Nevertheless, preharvest 1-MCP applied as a liquid spray to trees is a novel treatment for maintaining fruit quality throughout the postharvest in some crops and can be a very useful tool for improving handling operations in packing houses. This chapter aims to provide an overview of not only employing 1-MCP as a preharvest treatment in different crops, but also of its effect on the biochemical and physico-chemical parameters that influence fruit postharvest quality, storage capacity, and chilling injury development. It also intends to address the main factors related to the preharvest 1-MCP application effect, such as application time, optimum concentrations, and its combination with other preharvest treatments.