Improving the lipid recovery from wet oleaginous microorganisms using different pretreatment techniques

Howlader, Shamim; Rai, Neeraj; French, W. Todd

doi:10.1016/j.biortech.2018.07.092

Cited by 23 publications

(14 citation statements)

References 140 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Firstly, the total lipid content of our biomass was determined according to the conventional modified Folch's method [21]. According to some studies, surfactants were used to ease the disruption of the algae cell wall and enhance the lipid yield [22,23]. Aside from its role in facilitating microalgae cell disruption, surfactants have been proven to be viable alternatives to some environmentally toxic organic solvents [7].…”

Section: Pretreatment Of Microalgae Biomassmentioning

confidence: 99%

A Comprehensive Study on DES Pretreatment Application to Microalgae for Enhanced Lipid Recovery Suitable for Biodiesel Production: Combined Experimental and Theoretical Investigations

2023

View full text Add to dashboard Cite

Cell wall disturbance is an important step in the downstream process of improving the efficiency of lipid extraction from microalgae. Surfactants have been proven to be efficient alternatives to organic solvents in the extraction process. In this study, an effective approach involving deep eutectic solvent (DES) (choline chloride and carboxylic acids) treatment supplemented with surfactants has been developed to disrupt the cell walls of microalgae and increase the extraction of lipids suitable for biodiesel production. A combination of polar and non-polar solvents (ethyl acetate and n-butanol) was used for the lipid extraction process. Microalgae biomass pretreated with choline chloride malonic acid supplemented with the surfactant hexadecyl trimethylammonium chloride (HTAC) showed the best results, improving lipid extraction by 12.365%. Further elucidation of the detailed mechanism behind the cell disruption of the microalga wall by DES was achieved using density functional theory (DFT) methods. The DFT calculations revealed that hydrogen bonds between the chloride ion of the DES and hydrogen bond donor (HBD) molecules are key factors dominating the destruction of the cell wall structure of Chlorella pyrenoidosa. The optimization of lipid extraction was performed through a single-factor experiment, which included the effects of different variables (time, temperature, dosage of surfactant, and ratio of n-butanol to ethyl acetate). An extraction period of 60 min at 80 °C with a surfactant concentration of 0.5% at a 1:2 ratio of n-butanol to ethyl acetate was found to produce the maximum lipid yield (16.97%). Transesterification reactions were used to obtain fatty acid methyl esters from the optimized extracted lipids. Thus, it was determined that C16:0 (20.04%), C18:2 (29.95%), and C18:3 (21.21%) were the most prevalent fatty acids. The potential for producing biodiesel from C. pyrenoidosa was validated by the high yields of C18 fatty acid methyl esters, and the properties of biodiesel are within the European and US standards.

show abstract

Section: Pretreatment Of Microalgae Biomassmentioning

confidence: 99%

A Comprehensive Study on DES Pretreatment Application to Microalgae for Enhanced Lipid Recovery Suitable for Biodiesel Production: Combined Experimental and Theoretical Investigations

2023

View full text Add to dashboard Cite

show abstract

“…One of the key features of microalgae is the rigidity of its cell wall, while damage to their cell wall permits further CO 2 -SF to reach the selective compound. There are several cell disruption methods, such as mechanical, physical, chemical, and enzymatic approaches [3,19,43,44,45]. Lee et al [17] reported a study to compare different microalgal biomass pretreatment methods and suggested that mechanical disruption methods be considered as highly energy efficient approaches when conducted under laboratory conditions.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, by combining different sets of pressure and temperature, it is possible to obtain extracts with different compositions [54]. Also, Cheung, [44] reported that, under low pressures, more SUFA are extracted. On the contrary, as the pressure increased, the proportion of unsaturated FAs increased in the extracted phase from Hypneacharoides (Red Seaweed).…”

Section: Resultsmentioning

confidence: 99%

Eicosapentaenoic Acid Extraction from Nannochloropsis gaditana Using Carbon Dioxide at Supercritical Conditions

et al. 2019

View full text Add to dashboard Cite

This research shows that carbon dioxide supercritical fluid (CO2-SF) is an emerging technology for the extraction of high interest compounds for applications in the manufacturing of pharmaceuticals, nutraceuticals, and cosmetics from microalgae. The purpose of this study is to recover fatty acids (FAs) and, more precisely, eicosapentaenoic acid (EPA) from Nannochloropsis gaditana biomass by CO2-SF extraction. In the paper, the effect of mechanical pre-treatment was evaluated with the aim of increasing FAs recovery. Extraction was performed at a pressure range of 250–550 bars and a CO2 flow rate of 7.24 and 14.48 g/min, while temperature was fixed at 50 or 65 °C. The effect of these parameters on the extraction yield was assessed at each extraction cycle, 20 min each, for a total extraction time of 100 min. Furthermore, the effect of biomass loading on EPA recovery was evaluated. The highest EPA extraction yield, i.e., 11.50 mg/g, corresponding to 27.4% EPA recovery, was obtained at 65 °C and 250 bars with a CO2 flow rate of 7.24 g/min and 1.0 g biomass loading. The increased CO2 flow rate from 7.24 to 14.48 g/min enhanced the cumulative EPA recovery at 250 bars. The purity of EPA could be improved by biomass loading of 2.01 g, even if recovery was reduced.

show abstract

“…The physicochemical composition is the key factor for the valorization of the biomass growth during the production of erythritol. Several studies have considered the potential of Y. lipolytica in multiple applications including the utilization as an oleaginous platform for obtaining different fatty acids that can be used in biodiesel production (Patel et al 2016 ; Howlader et al 2018 ) . Besides, in 2019, the European Food Safety Authority (EFSA) recognized Y. lipolytica as a dietary supplement targeted to a general population from 3 years of age onwards (Turck et al 2019 ).…”

Section: Opportunities For the Production Of Erythritol In A Circular Economy Contextmentioning

confidence: 99%

From the culture broth to the erythritol crystals: an opportunity for circular economy

Daza-Serna

Serna-Loaiza

Masi

et al. 2021

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The reduction of sugar intake by adults has been stated by the World Health Organization as an important strategy to reduce the risk of non-communicable diseases. Erythritol is a four-carbon sugar alcohol that is considered as a highly suitable substitution for sucrose. This review article covers approaches for the separate stages of the biotechnological production of erythritol from cultivation to the downstream section. The first part focuses on the cultivation stage and compares the yields of erythritol and arising by-products achieved with different types of substrates (commercial versus alternative ones). The reported numbers obtained with the most prominently used microorganisms in different cultivation methods (batch, fed-batch or continuous) are presented. The second part focuses on the downstream section and covers the applied technologies for cell removal, recovery, purification and concentration of erythritol crystals, namely centrifugation, membrane separation, ion and preparative chromatography, crystallization and drying. The final composition of the culture broth and the preparative chromatography separation performance were identified as critical points in the production of a high-purity erythritol fraction with a minimum amount of losses. During the review, the challenges for a biotechnological production of erythritol in a circular economy context are discussed, in particular regarding the usage of sustainable resources and minimizing waste streams. Key points • Substitution of sucrose by erythritol can be a step towards a healthier society • Biotechnological production of erythritol should follow a circular economy concept • Culture broth composition and preparative chromatography are keys for downstreaming • Substrate, mother liquor and nutrients are challenges for circular economy

show abstract

Improving the lipid recovery from wet oleaginous microorganisms using different pretreatment techniques

Cited by 23 publications

References 140 publications

A Comprehensive Study on DES Pretreatment Application to Microalgae for Enhanced Lipid Recovery Suitable for Biodiesel Production: Combined Experimental and Theoretical Investigations

A Comprehensive Study on DES Pretreatment Application to Microalgae for Enhanced Lipid Recovery Suitable for Biodiesel Production: Combined Experimental and Theoretical Investigations

Eicosapentaenoic Acid Extraction from Nannochloropsis gaditana Using Carbon Dioxide at Supercritical Conditions

From the culture broth to the erythritol crystals: an opportunity for circular economy

Contact Info

Product

Resources

About