Improving the protective ability of lignin against vascular and neurological development in BPAF-induced zebrafish by high-pressure homogenization technology

Deng, Junping; Gu, Jie; Zhao, Xiaoxue; Yan, Bowen; Wang, Lei; Ji, Guixiang; Huang, Caoxing

doi:10.1016/j.ijbiomac.2023.123356

Cited by 8 publications

(2 citation statements)

References 59 publications

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“…To date, the highest concentration (100 mg/L) used for the experimental analyses is greater than the lignin NPs concentration reported in literature, tested for biomedical purposes. 25 …”

Section: Discussionmentioning

confidence: 99%

“… 23 , 24 Zebrafish has been employed so far as a model organism for in vivo illness modeling and evaluation of medicine and plant-derived extractives since the hematopoietic system and the immune cell types are similar to the human ones. 25 Furthermore, zebrafish has been largely implied as an inflammation model, since it is highly influenced by different types of external injuries, and consequently more susceptible to inflammation. 26 …”

Section: Introductionmentioning

confidence: 99%

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Exploring the Effects of Lignin Nanoparticles in Different Zebrafish Inflammatory Models

Bragato,

Persico,

Ferreres

et al. 2024

IJN

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Purpose Lignin is the most abundant source of aromatic biopolymers and has gained interest in industrial and biomedical applications due to the reported biocompatibility and defense provided against bacterial and fungal pathogens, besides antioxidant and UV-blocking properties. Especially in the form of nanoparticles (NPs), lignin may display also antioxidant and anti-inflammatory activities. Methods To evaluate these characteristics, sonochemically nano-formulated pristine lignin (LigNPs) and enzymatically-phenolated one (PheLigNPs) were used to expose zebrafish embryos, without chorion, at different concentrations. Furthermore, two different zebrafish inflammation models were generated, by injecting Pseudomonas aeruginosa lipopolysaccharide (LPS) and by provoking a wound injury in the embryo caudal fin. The inflammatory process was investigated in both models by qPCR, analyzing the level of genes as il8, il6, il1β, tnfα, nfkbiaa, nfk2 , and ccl34a.4 , and by the evaluation of neutrophils recruitment, taking advantage of the Sudan Black staining, in the presence or not of LigNPs and PheLigNPs. Finally, the Wnt/β-catenin pathway, related to tissue regeneration, was investigated at the molecular level in embryos wounded and exposed to NPs. Results The data obtained demonstrated that the lignin-based NPs showed the capacity to induce a positive response during an inflammatory event, increasing the recruitment of cytokines to accelerate their chemotactic function. Moreover, the LigNPs and PheLigNPs have a role in the resolution of wounds, favoring the regeneration process. Conclusion In this paper, we used zebrafish embryos within 5 days post fertilization (hpf). Despite being an early-stage exemplary, the zebrafish embryos have proven their potential as predicting models. Further long-term experiments in adults will be needed to explore completely the biomedical capabilities of lignin NPs. The results underlined the safety of both NPs tested paved the way for further evaluations to exploit the anti-inflammatory and pro-healing properties of the lignin nanoparticles examined.

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“…To date, the highest concentration (100 mg/L) used for the experimental analyses is greater than the lignin NPs concentration reported in literature, tested for biomedical purposes. 25 …”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exploring the Effects of Lignin Nanoparticles in Different Zebrafish Inflammatory Models

Bragato,

Persico,

Ferreres

et al. 2024

IJN

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Advancing Sustainable Building Materials: Reducing Formaldehyde Emissions in Medium Density Fiber Boards with Lignin Nanoparticles

Dorieh,

Pahlavan,

Hájková

et al. 2024

Advanced Sustainable Systems

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The pressing need to develop eco‐friendly polymer materials for building applications has led to increased interest in modifying existing polymer systems. In this study, a sustainable approach to augmenting urea‐formaldehyde (UF) resins, widely employed in wood‐based panels is introduced. Addressing this, formaldehyde‐scavenger demethylated lignin nanoparticles into UF resins, aiming to produce a green and enhanced medium‐density‐fiberboard (MDF) with minimal formaldehyde emissions is incorporated. The results indicate that increasing concentrations of demethylated lignin nanoparticles in the UF adhesive, there is not only a reduction in formaldehyde emissions from MDF composites but also a significant decrease in thickness swelling. The highest reduction in formaldehyde emission is observed in the MDF composite prepared with UF resin containing 7% lignin nanoparticles (UF‐7NL), with an emission of 2.9 mg/100 g, marking a substantial decrease of 74% compared to emission of 11.2 mg/100 g from neat resin. Importantly, this reduction does not compromise physical and mechanical properties of the MDF; they remain comparable to boards bonded with unmodified UF. Molecular modeling revealed that lignin effectively traps formaldehyde, incorporating it as ‐CH2OH groups, leading to a notable decrease in formaldehyde emission from MDF. This approach offers an eco‐friendly modification to a common polymer, showcasing lignin nanoparticles as innovative additives.

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Biosynthesizing lignin dehydrogenation polymer to fabricate hybrid hydrogel composite with hyaluronic acid for cartilage repair

Pei,

Yusufu,

Zhan

et al. 2023

Adv Compos Hybrid Mater

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Improving the protective ability of lignin against vascular and neurological development in BPAF-induced zebrafish by high-pressure homogenization technology

Cited by 8 publications

References 59 publications

Exploring the Effects of Lignin Nanoparticles in Different Zebrafish Inflammatory Models

Exploring the Effects of Lignin Nanoparticles in Different Zebrafish Inflammatory Models

Advancing Sustainable Building Materials: Reducing Formaldehyde Emissions in Medium Density Fiber Boards with Lignin Nanoparticles

Biosynthesizing lignin dehydrogenation polymer to fabricate hybrid hydrogel composite with hyaluronic acid for cartilage repair

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