Improving the quality of neurosteroid induced anaesthesia in rodents

Abstract

Declaration by author iv Publications during candidature v Publications included in this thesis v Contributions by others to the thesis vi Statement of parts of the thesis submitted to qualify for the award of another degree vi Research involving human or animal subjects (Ethics approval) vi Acknowledgements vii Financial support ix Keywords ix Australian and New Zealand Standard Research Classifications (ANZSRC) ix Fields of Research (FoR) Classification ix Table of contents x List of figures xiii List of tables xv List of abbreviations used in the thesis xvi Chapter 1 Laboratory animal anaesthesia 1 Safety in laboratory rodent anaesthesia 3 Limitations of current anaesthetic regimes 3 Current approaches to anaesthetic drug development 4 Neurosteroids as anaesthetics 5 Alfaxalone 7 Neuromotor excitation during anaesthesia 8 Glutamatergic transmission in motor neurons 9 Possible involvement of transient receptor potential (TRP) channels in motor neuron excitation 9 Rationale and aim of study 11 Materials and methods 12 xi Chapter 2

Alfaxalone Causes Reduction of Glycinergic IPSCs, but Not Glutamatergic EPSCs, and Activates a Depolarizing Current in Rat Hypoglossal Motor Neurons

“…The data presented in this article includes content which first appeared in CL’s and PT’s doctoral theses (Lau, 2013; Thakre, 2018); these theses represent the only medium in which this content has appeared in, are in line with The University of Queensland policy, and can be accessed online at https://doi.org/10.14264/uql.2016.1015 and https://doi.org/10.14264/uql.2018.709. Jurox Australia provided gifts of alfaxalone and HPCD to MB.…”