Improving the reliability and ecological validity of pharmaceutical risk assessment: Turquoise killifish (<i>Nothobranchius furzeri</i>) as a model in behavioral ecotoxicology

Thoré, Eli S.J.; Steenaerts, Laure; Philippe, Charlotte; Grégoir, Arnout; Brendonck, Luc; Pinceel, Tom

doi:10.1002/etc.4301

Cited by 25 publications

(21 citation statements)

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“…Spontaneous locomotor activity and boldness were assessed using an open field assay (cf. Ansai et al, 2016;Thoré et al, 2018aThoré et al, , 2018b. Fish were individually transferred to a 2.5 l tank (18 cm long × 12 cm wide × 11.5 cm high) with a water level of 2 cm (water volume of 0.5 l).…”

Section: Open Field Assaymentioning

confidence: 99%

“…An emergence test was used to study fish boldness and exploration tendency (cf. Thoré et al, 2018aThoré et al, , 2018b…”

Section: Emergence Testmentioning

confidence: 99%

“…When the fish initiated feeding (nonfeeding fish were excluded), a suspended, weighted 15 ml falcon tube (opaque, beak-shaped) was dropped and allowed to touch the water surface to simulate an avian predator attack (cf. Bell & Sih, 2007;Hedgespeth et al, 2016;Thoré et al, 2018aThoré et al, , 2018b. Subsequently, the time to resume feeding was recorded during the next 30 min.…”

Section: Life-skills Testmentioning

confidence: 99%

“…Because of the inconveniently long lifespan and generation time of classic fish models, full life-cycle and multigenerational assays (e.g., OECD guidelines for the testing of chemicals, OECD, 234, 240) are highly resource and time expensive and are therefore hardly performed for regulatory or research purposes (Ankley & Villeneuve, 2006). After an initial screening of congeneric species such as N. rachovii (van der Hoeven et al, 1982) and N. guentheri (Shedd et al, 1999), N. furzeri has been introduced as a more favourable model organism to facilitate such research agendas (Philippe et al, 2018a(Philippe et al, , 2018b(Philippe et al, , 2018cThoré et al, 2018a). Elaborate protocols for acute and chronic ecotoxicity testing with N. furzeri have been developed (Philippe et al, 2018b).…”

Section: Introductionmentioning

confidence: 99%

“…Elaborate protocols for acute and chronic ecotoxicity testing with N. furzeri have been developed (Philippe et al, 2018b). Recently, traditional ecotoxicological testing has been expanded by also including sensitive behavioural responses to more accurately assess the environmental impact of pollution, especially with regard to pharmaceutical contaminants (Thoré et al, 2018a(Thoré et al, , 2019(Thoré et al, , 2020. To date, however, a lack of fundamental baseline data and the "behavioural norm" of fish models hampers rigorous ecological risk assessment of chemical compounds, and examining how environmental conditions affect baseline behavioural expression is imperative (Harris et al, 2014;Thoré et al, 2018a;Tanoue et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Conspecific density and environmental complexity impact behaviour of turquoise killifish (Nothobranchius furzeri)

Thoré

Brendonck

Pinceel

2020

Journal of Fish Biology

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Fish models are essential for research in many biological and medical disciplines. With a typical lifespan of only 6 months, the Turquoise killifish (Nothobranchius furzeri) was recently established as a time-and cost-efficient model to facilitate whole-life and multigenerational studies in several research fields, including behavioural ecotoxicology. Essential information on the behavioural norm and on how laboratory conditions affect behaviour, however, is deficient. In the current study, we examined the impact of the social and structural environment on a broad spectrum of behavioural endpoints in N. furzeri. While structural enrichment affected only fish boldness and exploratory behaviour, fish rearing density affected the total body length, locomotor activity, boldness, aggressiveness and feeding behaviour of N. furzeri individuals. Overall, these results contribute to compiling a behavioural baseline for N. furzeri that increases the applicability of this new model species. Furthermore, our findings will fuel the development of improved husbandry protocols to maximize the welfare of N. furzeri in a laboratory setting.

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Section: Open Field Assaymentioning

confidence: 99%

“…An emergence test was used to study fish boldness and exploration tendency (cf. Thoré et al, 2018aThoré et al, , 2018b…”

Section: Emergence Testmentioning

confidence: 99%

Section: Life-skills Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Conspecific density and environmental complexity impact behaviour of turquoise killifish (Nothobranchius furzeri)

Thoré

Brendonck

Pinceel

2020

Journal of Fish Biology

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A Critical Review of the Availability, Reliability, and Ecological Relevance of Arctic Species Toxicity Tests for Use in Environmental Risk Assessment

Eldridge

Jourdan

Hanson

2021

Enviro Toxic and Chemistry

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There is a pressing need to understand the impact of contaminants on Arctic ecosystems; however, most toxicity tests are based on temperate species, and there are issues with reliability and relevance of bioassays in general. Together this may result in an underestimation of harm to Arctic organisms and contribute to significant uncertainty in risk assessments. To help address these concerns, a critical review to assess reported effects for these species, quantify methodological and endpoint relevance gaps, and identify future research needs for testing was performed. We developed uniform criteria to score each study, allowing an objective comparison across experiments to quantify their reliability and relevance. We scored a total of 48 individual studies, capturing 39 tested compounds, 73 unique Arctic test species, and 95 distinct endpoints published from 1975 to 2021. Our analysis shows that of 253 test substance and species combinations scored (i.e., a unique toxicity test), 207 (82%) failed to meet at least one critical study criterion that contributes to data reliability for use in risk assessment. Arctic-focused toxicity testing needs to ensure that exposures can be analytically confirmed, include environmentally realistic exposure scenarios, and report test methods more thoroughly. Significant data gaps were identified as related to standardized toxicity testing with Arctic species, diversity of compounds tested with these organisms, and the inclusion of ecologically relevant sublethal and chronic endpoints assessed in Arctic toxicity testing. Overall, there needs to be ongoing improvement in test conduction and reporting in the scientific literature to support effective risk assessments in an Arctic context.

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Impact of Fluoxetine on Herbivorous Zooplankton and Planktivorous Fish

Grzesiuk

Gryglewicz

Bentkowski

et al. 2022

Enviro Toxic and Chemistry

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The contamination of freshwater environments by pharmaceuticals is a growing problem. Modern healthcare uses nearly 3000 substances, many of which are designed to work at low dosages and act on physiological systems that have been evolutionarily conserved across taxa. Because drugs affect the organisms from different trophic levels, pharmaceutical pollution is likely to disturb species interactions. However, such effects are still only poorly understood. We investigated the impacts of environmentally relevant concentrations of the common drug fluoxetine (Prozac), an increasingly common contaminant of European waters, on predation behavior of crucian carp (Carassius carassius), a common planktivorous European fish, and the somatic growth of its prey, the water flea (Daphnia magna), a widespread planktonic crustacean. We exposed these two organisms to environmentally relevant levels of fluoxetine (360 ng L−1): the fish for 4 weeks and the water fleas for two generations. We tested the growth of the daphnids and the hunting behavior (reaction distance at which fish attacked Daphnia and feeding rate) of the fish under drug contamination. We found that Daphnia exposed to fluoxetine grew larger than a nonexposed cohort. The hunting behavior of C. carassius was altered when they were exposed to the drug; the reaction distance was shorter, and the feeding rate was slower. These effects occurred regardless of Daphnia size and the treatment regime they were subjected to. Our results suggest that contamination of freshwater environments with fluoxetine can disrupt the top‐down ecological control of herbivores by reducing the hunting efficiency of fish and, as a consequence, may lead to increases in cladoceran population numbers. Environ Toxicol Chem 2023;42:385–392. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

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Improving the reliability and ecological validity of pharmaceutical risk assessment: Turquoise killifish (Nothobranchius furzeri) as a model in behavioral ecotoxicology

Cited by 25 publications

References 58 publications

Conspecific density and environmental complexity impact behaviour of turquoise killifish (Nothobranchius furzeri)

Conspecific density and environmental complexity impact behaviour of turquoise killifish (Nothobranchius furzeri)

A Critical Review of the Availability, Reliability, and Ecological Relevance of Arctic Species Toxicity Tests for Use in Environmental Risk Assessment

Impact of Fluoxetine on Herbivorous Zooplankton and Planktivorous Fish

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