Improving the Stability of Cold-Adapted Enzymes by Immobilization

Abstract

Cold-adapted enzymes have gained considerable attention as biocatalysts that show high catalytic activity at low temperatures. However, the use of cold-adapted enzymes at ambient temperatures has been hindered by their low thermal stabilities caused by their inherent structural flexibilities. Accordingly, protein engineering and immobilization have been employed to improve the thermal stability of cold-adapted enzymes. Immobilization has been shown to increase the thermal stability of cold-adapted enzymes at the critical temperatures at which denaturation begins. This review summarizes progress in immobilization of cold-adapted enzymes as a strategy to improve their thermal and organic solvent stabilities.

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

“…It also intends to improve the stability of the enzyme (Chandorkar et al, 2014; Lee et al, 2017). To study the effect of entrapment on lipase activity, entrapment of B. cereus HSS cells was carried out in 3% sodium alginate.…”

A Bacterial Machinery for Surface Displayed Enzymes

“…However, generally enzymes are used in their pure form and the cost for the enzymes may cause problems in their co-effectiveness. In this regard, there have been studies to immobilize enzymes and protein on solid supports to provide a longer lifetime and better durability for them [21]. In a whole cell system these optimizations can be achieved through engineering of the cellular genetic regulation systems [22].…”

Cold-active enzymes in food biotechnology: An updated mini review

“…Even though the cold-active enzymes have high specific activity but small half-life and low thermal stability are the major drawback that limits utilization of such enzymes at industrial level. To increase thermal and solvent stability of cold-active enzymes, different methods of enzyme immobilization are recommended by various researchers [31]. Along with immobilization, different molecular tactics, for example, protein engineering, recombinant DNA technology and metagenomic approach could also be used to cope the commercial expectations and development of unique cold-active enzymes.…”