As a country, Serbia is paving the path for membership in the European Union - facing many complex challenges, where a significant place belongs to the solution of environmental protection and sustainable development problems. Approaching these challenges effectively contributes to regional cooperation in the field of resource utilization for sustainable development, as well as for the improvement of the health of all citizens. In the scope of economic branches, specific forms of tourism business: ecotourism, rural tourism, health, and recreational tourism, could be of great importance for financing the protection of sensitive areas, i.e., the socio-economic development of the population in Serbia. The subject of the chapter is the research and development of regional cooperation that affect the tourism sector of Serbia, with an emphasis on health tourism. The aim of improving regional cooperation is to achieve a more successful business in the field of tourism, especially in specific tourism branches, such as health and recreational tourism.