Impurity profiling of l-asparagine monohydrate by ion pair chromatography applying low wavelength UV detection

Schilling, Klaus; Amstalden, Maria Cecilia; Meinel, Lorenz; Holzgrabe, Ulrike

doi:10.1016/j.jpba.2016.08.035

Cited by 5 publications

(4 citation statements)

References 19 publications

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“…A cation exchange column [ 28 ], reverse-phase column [ 29 , 30 , 31 , 32 ], hydrophilic interaction liquid chromatography (HILIC)-mode column [ 33 , 34 ], and mix-mode column [ 35 ] are used for HPLC separation. In addition, ion-pair mobile phases are applied to detect amino acid impurities with a reverse-phase column [ 36 , 37 , 38 , 39 ] or HILIC-mode column [ 40 ]. Most amino acids and impurities that lack a chromophore are detected by ultraviolet (UV) detection at a low wavelength near UV 210 nm [ 3 , 29 , 30 , 33 , 36 ] or charged aerosol detection (CAD) [ 29 , 37 , 38 , 39 , 40 ].…”

Section: Analytical Methods To Determine Impurities In Amino Acidsmentioning

confidence: 99%

“…In addition, ion-pair mobile phases are applied to detect amino acid impurities with a reverse-phase column [ 36 , 37 , 38 , 39 ] or HILIC-mode column [ 40 ]. Most amino acids and impurities that lack a chromophore are detected by ultraviolet (UV) detection at a low wavelength near UV 210 nm [ 3 , 29 , 30 , 33 , 36 ] or charged aerosol detection (CAD) [ 29 , 37 , 38 , 39 , 40 ]. For more sensitive detection, pre-column derivatization with fluorescence reagents and fluorescence detection (FL) are used.…”

Section: Analytical Methods To Determine Impurities In Amino Acidsmentioning

confidence: 99%

“…An ion-pair mobile phase and reverse-phase column are often used in combination. Schilling et al reported an analytical method using a C18 column with an ion-pair reagent (sodium octanesulfonate) and applied it to asparagine analysis [ 36 ]. Qiu et al reported the use of trifluoroacetic acid and heptafluorobutyric acid (HFBA) as ion-pair reagents [ 37 ].…”

Section: Analytical Methods To Determine Impurities In Amino Acidsmentioning

confidence: 99%

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Analytical Chemistry of Impurities in Amino Acids Used as Nutrients: Recommendations for Regulatory Risk Management

et al. 2022

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Proteinogenic amino acids are natural nutrients ingested daily from standard foods. Commercially manufactured amino acids are added to a wide range of nutritional products, including dietary supplements and regular foods. Currently, the regulatory risk management of amino acids is conducted by means of setting daily maximum limits of intake. However, there have been no reported adverse effects of amino acid overdosing, while impurities in low-quality amino acids have been identified as causative agents in several health hazard events. This paper reviews the analytical chemistry of impurities in amino acids and highlights major variations in the purity of commercial products. Furthermore, it examines the international standards and global regulatory risk assessment of amino acids utilized in dietary supplements and foods, recommending (1) further research on analytical methods that can comprehensively separate impurities in amino acids, and (2) re-focusing on the regulatory risk management of amino acids to the analytical chemistry of impurities.

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Section: Analytical Methods To Determine Impurities In Amino Acidsmentioning

confidence: 99%

Section: Analytical Methods To Determine Impurities In Amino Acidsmentioning

confidence: 99%

Section: Analytical Methods To Determine Impurities In Amino Acidsmentioning

confidence: 99%

See 1 more Smart Citation

Analytical Chemistry of Impurities in Amino Acids Used as Nutrients: Recommendations for Regulatory Risk Management

et al. 2022

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“…Over the last decades, some efforts have been made to develop methods for determining L-Asp in serum samples. Standard instrumental techniques such as ion-pair chromatography [2], spectrophotometry [3], and colorimetric assay [4] have been used for the determination of L-Asp; however, these methods are often sophisticated, time-consuming, and very expensive.…”

Section: Introductionmentioning

confidence: 99%

The Development of an Amperometric Enzyme Biosensor Based on a Polyaniline‐Multiwalled Carbon Nanocomposite for the Detection of a Chemotherapeutic Agent in Serum Samples from Patients

2021

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Polyaniline was electrochemically polymerized onto the platinum electrode modified with a mixture of Nafion and multiwalled carbon nanotube (PANI/MWCNT/Nf/Pt) to detect ammonium ion. The nanobiosensor (ASNase/PANI/MWCNT/Nf/Pt) was then prepared by immobilizing L-asparaginase (L-ASNase) on the PANI/MWCNT/Nf nanocomposite. The prepared nanobiosensor was used for the rapid and sensitive detection of serum concentration of the anticancer agent L-asparagine (L-Asp) during chemotherapy. The nanobiosensor has dynamic ranges of zero to 180 μM. The sensitivity of the nanobiosensor was 0.829 μA μM−1 cm−2, and the response time was less than 30 s. The detection limit was 140 nM of L-Asp. The Michaelis–Menten constant ( K m ) was measured to be 36.2 mM. The nanobiosensor was successfully applied for the determination of L-Asp in the blood samples of leukemia patients.

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Method transfer from ion pair chromatography to charged aerosol detector-compatible mixed-mode chromatography: A case study using carbocisteine

Walther

Oliver

Holzgrabe

2021

Journal of Chromatography Open

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Impurity profiling of l-asparagine monohydrate by ion pair chromatography applying low wavelength UV detection

Cited by 5 publications

References 19 publications

Analytical Chemistry of Impurities in Amino Acids Used as Nutrients: Recommendations for Regulatory Risk Management

Analytical Chemistry of Impurities in Amino Acids Used as Nutrients: Recommendations for Regulatory Risk Management

The Development of an Amperometric Enzyme Biosensor Based on a Polyaniline‐Multiwalled Carbon Nanocomposite for the Detection of a Chemotherapeutic Agent in Serum Samples from Patients

Method transfer from ion pair chromatography to charged aerosol detector-compatible mixed-mode chromatography: A case study using carbocisteine

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