ImQuant — An image based fluorescence reader for quantitative lateral flow immunoassays

Chowdhury, Kaushik; Joseph, Jayaraj; Vasan, Jayaraman Kiruthi; Sivaprakasam, Mohanasankar

doi:10.1109/embc.2016.7591887

Cited by 5 publications

(2 citation statements)

References 8 publications

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“…In 2012, Mudanyali et al, demonstrated a cellphone-based rapid-diagnostic-test (RDT) reader platform that works with various lateral flow immuno-chromatographic assays and similar tests to sense the presence of a target analyte in a sample [ 16 ]. In 2015 and 2016, Chowdhury et al, developed a reader for quantitative lateral flow immunoassays using a line laser diode module to illuminate a lateral flow immunoassay test strip with fluorescent dye [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Use of the Taguchi Method to Optimize an Immunodetection System for Quantitative Analysis of a Rapid Test

Lin

Chang

2021

Diagnostics

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In this research, the Taguchi method was used to optimize the detection accuracy and reproducibility of an immunodetection system used for a quantitative analysis of a rapid test. Furthermore, the standard deviation (SD) and coefficient of variation (CV) between the theoretical value and the measured value of the self-made simulated rapid test became smaller, and the linearity became higher. The results thus indicated that the immunodetection system became more reliable. In the present research, a camera was used to capture an image containing the control line (C line) and the test line (T line) in the self-made simulated rapid test. The captured image was then analyzed, and the grayscales of the C line and T line were calculated. The Taguchi method was used to adjust the light intensity of the light-emitting diode (LED) and the camera parameters in the immunodetection system to determine the optimal parameters by which to optimize the performance of the immunodetection system. The goal of the present research was to obtain a measurement with a minimum SD and CV between the detected grayscales and the grayscales of the self-made simulated rapid test, thus indicating successful development of a practical, stable, and accurate immunodetection system. To mimic the color expression in an actual rapid test, the ratio of the red, green, blue (RGB) components of the self-made simulated rapid test had to be adjusted to closely fit the color expression of the actual rapid test. After the RGB ratio was set, the Taguchi method was used to optimize the parameters for the purpose of detection. When the optimal parameters were found, the signal-to-noise ratio (S/N ratio) had been increased from −12.89 dB to −10.91 dB, which means the accuracy of the color detection had been improved. Compared to the original detection system, the quality loss had been reduced to 33.1%.

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Section: Introductionmentioning

confidence: 99%

Use of the Taguchi Method to Optimize an Immunodetection System for Quantitative Analysis of a Rapid Test

Lin

Chang

2021

Diagnostics

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“…Therefore, to meet current clinical requirements, quantitative detection of targeted specimens has become necessary, and the development of rapid tests and image detection equipment has thus gained broad interest. Image-based immunochromatographic test strip readers have already been demonstrated by a few researchers [ 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Embedded Immunodetection System for Fecal Occult Blood

Lin

Chang

2021

Biosensors

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In this paper, a rapid test system with high sensitivity, linearity, and stability is presented for fecal occult blood (FOB) detection. The coloration results of the immune response are used as the basis for the determination of the detection target in combination with an immunochromatographic strip. The rapid test system can be used to detect and calculate the concentration of the sample, so detection of the immune coloration response is more accurate in a quantitative analysis. The system is composed of both hardware and software. The programs used for the analysis and programmed by Python include the main program, polarization calibration, QR Code decoding, Bluetooth transmission, and image processing. After verification of each part of the system, it was found that the rapid test system successfully detects from 0 ng/mL to 400 ng/mL of FOB with coefficients of variation (CV) below 3.7% and 1000 ng/mL with a CV only at 7.41%.

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PhoneQuant: A smartphone-based quantitative immunoassay analyser

Shah

Joseph

Sanne

et al. 2017

2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

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There is a vital need for portable and cost-effective point-of-care (PoC) testing technologies that provide reliable and rapid results. Lateral Flow Immunoassays (LFIA) are suitable PoC diagnostic tools with the potential for use in a wide variety of field applications ranging from uses in clinical diagnostics to aiding law enforcement. Quick and reliable diagnosis of non-communicable diseases (NCD) like diabetes is vital especially in developing countries like India where the burden of these diseases is very high and is increasing day by day. In this paper, we have presented the design of smartphone-based fully quantitative LFIA analyser, An automatic image processing algorithm is also described. A repeatability study was done with stable fluorescence reference cartridges. The Coefficient of Variation (CoV) for repeatability study was calculated and it was found to be good (<; 1.5%). The instrument was tested with blood samples to generate a calibration curve for Glycated Haemoglobin (HbA1c) with respect to standard lab instrument. Three different set of settings parameters were used in smartphone camera their calibration curve formed. All three curves were linearly correlated with R-value greater than 0.985. Finally, the calibration curve was validated through three HbA1c blood sample tests- for each sample, CoV was less than 5%. The PhoneQuant analyser is the portable cost-effective solution to traditional bulky LFIA analysers and it has good potential to be deployed at physician's desk or for in-home PoC testing for quick and reliable diagnosis.

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ImQuant — An image based fluorescence reader for quantitative lateral flow immunoassays

Cited by 5 publications

References 8 publications

Use of the Taguchi Method to Optimize an Immunodetection System for Quantitative Analysis of a Rapid Test

Use of the Taguchi Method to Optimize an Immunodetection System for Quantitative Analysis of a Rapid Test

Embedded Immunodetection System for Fecal Occult Blood

PhoneQuant: A smartphone-based quantitative immunoassay analyser

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