In‐depth analysis of low abundant proteins in bovine colostrum using different fractionation techniques

Nissen, Asger; Bendixen, Emøke; Ingvartsen, Klaus Lønne; Røntved, Christine M.

doi:10.1002/pmic.201200231

Cited by 47 publications

(65 citation statements)

References 62 publications

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“…Previous research identified milk proteins in bovine colostrum and mature milk (D'Alessandro et al, 2011;Nissen et al, 2012;Sacerdote et al, 2013), and the quantitative differences in the milk proteome between bovine colostrum and mature milk (Stelwagen et al, 2009;Zhang et al, 2015). So far, however, variation has not been studied in these low-abundant proteins in mature bovine milk over the whole lactation (from early to the end of lactation).…”

Section: Introductionmentioning

confidence: 94%

Perspective on calf and mammary gland development through changes in the bovine milk proteome over a complete lactation

Zhang

Boeren

Hageman

et al. 2015

Journal of Dairy Science

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Milk contains all the nutrients for the growth and development of the neonate. However, milk composition is not constant during lactation. To study the changes of the milk proteome over lactation, filter-aided sample preparation combined with dimethyl labeling followed by liquid chromatography tandem mass spectrometry was used to identify and quantify milk proteins from 4 cows. A total of 229 proteins were identified, of which 219 were quantified. An 80% overlap was found in identified and quantified proteins between the 4 individual cows during lactation. Over lactation, the number of quantified proteins changed slightly (less than 10%), whereas the concentration of proteins changed considerably. Transport proteins involved in lipid synthesis (fatty acid-binding protein, perilipin-2, butyrophilin) increased, whereas proteins related to cholesterol transport (apolipoprotein E) decreased. The changes of lipid synthesis proteins are in accordance with the increased milk fat yield over lactation, indicating the increase of de novo mammary fatty acid synthesis as lactation advances. The high abundance of immune-related proteins in early lactation indicates the important role of these proteins for immune system development of calves. The increase in immune-related proteins (immunoglobulins, osteopontin, lactoferrin) and the decrease of proteins related to milk component synthesis (α-lactalbumin, β-lactoglobulin, fatty acid-binding protein, perilipin-2, butyrophilin) in late lactation can be associated with the protection of the mammary gland. In conclusion, the changes of proteins with different biological functions reflect not only the changing needs of calves but also the development and protection of the mammary gland over lactation.

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Section: Introductionmentioning

confidence: 94%

Perspective on calf and mammary gland development through changes in the bovine milk proteome over a complete lactation

Zhang

Boeren

Hageman

et al. 2015

Journal of Dairy Science

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“…Previously, we published 2 comprehensive proteomebased studies of the of the proteins present in bovine colostrum sampled from the first milking after calving and in milk sampled on d 10 (Nissen et al, 2012(Nissen et al, , 2013. These results allow us to investigate whether biologically active proteins other than immunoglobulins were exclusive to colostrum or present in higher concentrations in colostrum than in milk.…”

Section: Introductionmentioning

confidence: 99%

Colostrum and milk protein rankings and ratios of importance to neonatal calf health using a proteomics approach

Nissen

Andersen

Bendixen

et al. 2017

Journal of Dairy Science

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Administration of colostrum to the newborn calf before gut closure is pivotal to its health, because of the transfer of passive immunity. Traditionally, passive immunity has been attributed to the transfer of immunoglobulins although it is increasingly clear that multiple other factors contribute, including innate immune proteins, developmental factors, immunomodulatory factors, and the presence of cellular immunity. The objective of this study was to produce a comprehensive comparison of the bovine colostrum proteome and the milk proteome by applying 2-dimensional liquid chromatography-tandem mass spectrometry. Further, the objectives were to rank proteins mutually and generate protein ratios from the spectral counts of the 2 proteomes and ELISA to gain insight into which proteins could be of most relevance to neonatal calf health. To obtain an in-depth picture of the bovine colostrum and milk proteome, we compared the contents of different fractions from bovine colostrum and milk from our 2 previous studies. A total of 140 colostrum fluid-phase proteins and 103 milk fluid-phase proteins were detected. In the cellular fraction, 324 and 310 proteins were detected in colostrum and milk, respectively. In total, 514 proteins were detected, of which 162 were in the fluid phase. Of these, 50 proteins were exclusively seen in colostrum, 13 were exclusively seen in milk, and 99 were common to colostrum and milk. Ranking of proteins mutually and calculating protein ratios based on spectral counts and ELISA resulted in new information on how proteins were associated with the fluid or cellular fraction of the samples. Interestingly, despite lower counts/concentrations than the classical proteins such as immunoglobulins, β-lactoglobulin, and lactotransferrin, several proteins appeared in higher or similar colostrum:milk spectral count ratios as these. Using this approach indicated, for example, that osteopontin, haptoglobin, milk amyloid A, and gelsolin may be interesting molecules to study in detail in their relation to calf health. Although the sensitivity, identification, and ranking of proteins varied between the 2 methods, and proteome analysis clearly suffers from low sensitivity, we believe that this idea and approach of generating ratios and ranking proteins can contribute new information and perspectives on how to prioritize the importance of multiple proteins, beyond immunoglobulins, in relation to neonatal calf health.

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“…Proteomics has been used to characterize protein changes in the transition from colostrum to milk in cattle [16,17]. Additionally, the study of low abundance proteins from different body fluids such as blood plasma, colostrum or milk is becoming increasingly relevant [16,18-21].…”

Section: Introductionmentioning

confidence: 99%

The effect of colostrum intake on blood plasma proteome profile in newborn lambs: low abundance proteins

et al. 2014

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BackgroundColostrum intake by newborn lambs plays a fundamental role in the perinatal period, ensuring lamb survival. In this study, blood plasma samples from two groups of newborn lambs (Colostrum group and Delayed Colostrum group) at 2 and 14 h after birth were treated to reduce the content of high abundance proteins and analyzed using Two-Dimensional Differential in Gel Electrophoresis and MALDI MS/MS for protein identification in order to investigate low abundance proteins with immune function in newborn lambs.ResultsThe results showed that four proteins were increased in the blood plasma of lambs due to colostrum intake. These proteins have not been previously described as increased in blood plasma of newborn ruminants by colostrum intake. Moreover, these proteins have been described as having an immune function in other species, some of which were previously identified in colostrum and milk.ConclusionsIn conclusion, colostrum intake modified the low abundance proteome profile of blood plasma from newborn lambs, increasing the concentration of apolipoprotein A-IV, plasminogen, serum amyloid A and fibrinogen, demonstrating that colostrum is essential, not only for the provision of immunoglobulins, but also because of increases in several low abundance proteins with immune function.

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In‐depth analysis of low abundant proteins in bovine colostrum using different fractionation techniques

Cited by 47 publications

References 62 publications

Perspective on calf and mammary gland development through changes in the bovine milk proteome over a complete lactation

Perspective on calf and mammary gland development through changes in the bovine milk proteome over a complete lactation

Colostrum and milk protein rankings and ratios of importance to neonatal calf health using a proteomics approach

The effect of colostrum intake on blood plasma proteome profile in newborn lambs: low abundance proteins

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