In-depth biochemical identification of a novel methyl parathion hydrolase from Azohydromonas australica and its high effectiveness in the degradation of various organophosphorus pesticides

Zhao, Sumao; Xu, Wei; Zhang, Wenli; Wu, Hao; Guang, Cuie; Mu, Wanmeng

doi:10.1016/j.biortech.2020.124641

Cited by 44 publications

(12 citation statements)

References 45 publications

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“…Therefore, Mn 2+ ions are not only necessary for the catalytic activity but also involved in OPAA114644 structure stabilization. The same phenomenon was observed in MPH from Azohydromonas australica [24] and prolidase in human [38].…”

Section: Metal Ions Analysissupporting

confidence: 76%

“…For OPAA114644 expression, the BL21 (DE3) strain with recombinant plasmid was cultivated in 200 mL Luria-broth (LB) medium containing 50 µg/mL kanamycin and 0.2 mM MnCl 2 at 37 • C. OPAA114644 expression was induced with 1 mM IPTG, and the protein was purified by nickel affinity chromatography with a 5 mL HisTrapTM HP column (GE Healthcare, Uppsala, Sweden as described previously [24]. The binding buffer was 50 mM Bis-Tris propane buffer, 0.5 M NaCl and 20 mM imidazole (pH 8.0), and the elution buffer was 50 mM Bis-Tris propane buffer, 0.5 M NaCl and 0.5 M imidazole (pH 8.0).…”

Section: Protein Expression and Purificationmentioning

confidence: 99%

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A Novel Organophosphorus Acid Anhydrolase from Deep Sea Sediment with High Degradation Efficiency for Organophosphorus Pesticides and Nerve Agent

Zheng

Wang

Qi³

et al. 2022

Microorganisms

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Organophosphorus compounds (OPCs), including highly toxic nerve agents and pesticides, have been used widely in agricultural and military applications. However, they have aroused widespread concern because they persistently pollute the environment and threaten human life. Organophosphorus acid anhydrolase (OPAA) is a promising enzyme that can detoxify OPCs. Here, a novel OPAA (OPAA114644) was isolated and characterized from deep-sea sediment (−3104 m). It exhibited excellent alkaline stability, and the loss of activity was less than 20% in the pH range 5.0–9.0, even after being incubated for 30 d at 4 °C. It also exhibited high salt tolerance, and its enzymatic activity increased by approximately fourfold in the presence of 20% NaCl (w/v). Additionally, OPAA114644 exhibited high degradation efficiency for soman, dichlorvos, paraoxon, coumaphos, and chlorpyrifos with a concentration of up to 250 mg/L, with the degradation rate being 100%, 100%, 100%, 80% and 51%, respectively, in 20 min under optimal conditions. Notably, OPAA114644 dissolved in different solutions, such as 20% NaCl, 1 mM SDS, 0.05% soap, 10% methanol, and tap water, could efficiently decontaminate the residual paraoxon on the surfaces of glasses, cotton tissues, and apples. These results indicate that OPAA114644 has excellent potential for the biodegradation and bioremediation of OPCs pollution and represents a real application of OPAA in the decontamination and detoxification of foods and clothes, and in the remediation of sites such as floors. Deep-sea sediment might also be an abundant resource for various functional microorganisms and enzymes.

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Section: Metal Ions Analysissupporting

confidence: 76%

Section: Protein Expression and Purificationmentioning

confidence: 99%

A Novel Organophosphorus Acid Anhydrolase from Deep Sea Sediment with High Degradation Efficiency for Organophosphorus Pesticides and Nerve Agent

Zheng

Wang

Qi³

et al. 2022

Microorganisms

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“…Despite the recent development of degradation means to deal with methyl parathion contaminants, such as methyl parathion hydrolase and ozone, the untargeted toxicity of organophosphorus remains unknown [ 19 , 20 ]. Zebrafish, due to high fecundity, rapid embryonic development, embryonic transparency, and high experimental yield, is becoming a popular model organism in environmental toxicity studies [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Methyl Parathion Exposure Induces Development Toxicity and Cardiotoxicity in Zebrafish Embryos

Chen

Wang

et al. 2023

Toxics

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Methyl parathion (MP) has been widely used as an organophosphorus pesticide for food preservation and pest management, resulting in its accumulation in the aquatic environment. However, the early developmental toxicity of MP to non-target species, especially aquatic vertebrates, has not been thoroughly investigated. In this study, zebrafish embryos were treated with 2.5, 5, or 10 mg/L of MP solution until 72 h post-fertilization (hpf). The results showed that MP exposure reduced spontaneous movement, hatching, and survival rates of zebrafish embryos and induced developmental abnormalities such as shortened body length, yolk edema, and spinal curvature. Notably, MP was found to induce cardiac abnormalities, including pericardial edema and decreased heart rate. Exposure to MP resulted in the accumulation of reactive oxygen species (ROS), decreased superoxide dismutase (SOD) activity, increased catalase (CAT) activity, elevated malondialdehyde (MDA) levels, and caused cardiac apoptosis in zebrafish embryos. Moreover, MP affected the transcription of cardiac development-related genes (vmhc, sox9b, nppa, tnnt2, bmp2b, bmp4) and apoptosis-related genes (p53, bax, bcl2). Astaxanthin could rescue MP-induced heart development defects by down-regulating oxidative stress. These findings suggest that MP induces cardiac developmental toxicity and provides additional evidence of MP toxicity to aquatic organisms.

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“…In order to improve crop yield in the eld, a series of pesticides, such as insecticides, fungicides, herbicides, cochides, rodenticides and nematocides, have been widely used, but pesticides can also cause damage to crops to varying degrees in the process of pesticides and pesticides [25][26][27]. The composition of pesticides and the concentration of pesticides applied will have a certain impact on the growth of crops [28,29].…”

Section: Validation Of the Degradation Ability Of Pinoxaden Degrading...mentioning

confidence: 99%

Degradation Characteristics of Pinoxaden by Acinetobacter and Prediction of Related Genes

Fang

Wei

Sun

et al. 2022

Preprint

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In order to improve crop yield per unit area, pinoxaden was widely used in weed control in the field to better degrade the residual pinoxaden in the soil. In this experiment, 14 strains were domesticated and isolated from soil samples using pinoxaden as the only carbon and nitrogen source, and the highly degraded strain was T4P20-1. The effects of different pH values, inoculation amount and culture time on the growth and degradation rate of strain T4P20-1 were studied. The optimal growth conditions of strain T4P20-1 were determined, and the degradation rate reached 92.23%. The morphological characteristics of strain T4P20-1 were determined by optical microscope. The strain T4P20-1 was identified as Acinetobacter Pittii by physiological and biochemical characteristics and 16S rDNA homology analysis, and the nucleic acid sequence was submitted to Gentbank to obtain the accession number MW350066. Through gene sequencing, the whole genome framework of strain T4P20-1 and database annotations of GO, KEGG, COG, CAZymes and PHI were obtained. Meanwhile, genes related to herbicide degradation were predicted by gene functional annotations. There was a negative correlation between herbicide and seed germination rate, bud length and root length of maize susceptible to subsequent crop. In conclusion, the strains screened in this study have certain remediation effects on soil contaminated by pinoxaden. It provides a new choice for the degradation of pinoxaden in the future. It can increase crop yields and reduce food security risks.

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In-depth biochemical identification of a novel methyl parathion hydrolase from Azohydromonas australica and its high effectiveness in the degradation of various organophosphorus pesticides

Cited by 44 publications

References 45 publications

A Novel Organophosphorus Acid Anhydrolase from Deep Sea Sediment with High Degradation Efficiency for Organophosphorus Pesticides and Nerve Agent

A Novel Organophosphorus Acid Anhydrolase from Deep Sea Sediment with High Degradation Efficiency for Organophosphorus Pesticides and Nerve Agent

Methyl Parathion Exposure Induces Development Toxicity and Cardiotoxicity in Zebrafish Embryos

Degradation Characteristics of Pinoxaden by Acinetobacter and Prediction of Related Genes

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