In-Gel Staining of Proteins in Native Poly Acryl Amide Gel Electrophoresis Using Tetrakis(4-sulfonato phenyl)porphyrin

Abstract

Protein identification in polyacrylamide gel electrophoresis (PAGE) requires post-electrophoretic steps like fixing, staining and destaining of the gel, which are time-consuming and cumbersome. We have developed a method for direct visualization of protein bands in PAGE using tetrakis(4-sulfonato phenyl)porphyrin (TPPS) as a dye without the need for any post electrophoretic steps, where separation and recovery of enzymes become much easier for further analysis. Activity staining was done to prove that the biochemical activity of the enzymes was preserved after electrophoresis.

Synthesis of Tailed Porphyrin Modified with Nicotinic Acid and Interactions with Human Serum Albumin

“…在生 物体系中, 金属卟啉配合物是许多酶的活性中心, [1][2][3][4][5] 在与血清白蛋白相互作用的研究中, 卟啉分子可与 血清白蛋白结合, 其聚集状态及在机体中的分布、 代谢等生理过程都受到血清白蛋白的影响. 因此, 研究金属卟啉与血清白蛋白相互作用的机理已引 起了人们的极大兴趣, 当前国内外的文献资料 [6][7][8][9][10][11][12] 中 也较多地报道了金属卟啉与牛血清白蛋白之间相 互作用的研究成果.…”

Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue

“…Coomassie blue staining has the following advantages: low cost, visual inspection, easy operation, convenient scanning procedure for image acquisition, better suitability for quantitative analysis than silver staining, and the capacity to allow possible modifications for fast or highly sensitive staining. Coomassie stain has become the key compound in Blue native PAGE in the last 10 years [13], [14]. However, it still requires a long staining time, and the relatively complicated ingredients make its use inconvenient.…”