No abstract

Protein identification in polyacrylamide gel electrophoresis (PAGE) requires post-electrophoretic steps like fixing, staining and destaining of the gel, which are time-consuming and cumbersome. We have developed a method for direct visualization of protein bands in PAGE using tetrakis(4-sulfonato phenyl)porphyrin (TPPS) as a dye without the need for any post electrophoretic steps, where separation and recovery of enzymes become much easier for further analysis. Activity staining was done to prove that the biochemical activity of the enzymes was preserved after electrophoresis.

The catalytic, optical and thermal properties are based on the size of the nanoparticles. Nickel oxide nanoparticles have unique optical property and excellent antibacterial activity. The present study aims for the synthesis of Nickel oxide nanoparticles (NiO NPs) using Sterculia foetida (S. foetida) leaf extract as reducing agent by solution combustion method. The synthesized Nickel oxide nanoparticles (NiO NPs) were confirmed by UV-Visible spectroscopy (UV) with the peak at 370 nm and at the temperature of 450 ± 10 °C, Fourier transform infrared (FTIR) wavelength was observed at 1418 cm–1 1027 cm–1 shows C–O stretching vibration and at 507 cm–1 vibration of Ni–O bond found. Crystalline structure and the formation of monoclinic phase revealed by Powder X-ray diffraction (PXRD) pattern, the percentage of nickel and oxygen of NiO NPs were confirmed with EDAX analysis. The Scanning electron microscopy (SEM) and Transmission electron microscope (TEM) images indicate the shape of Nickel oxide nanoparticles (NiO NPs) with the size range of 10–51 nm. Staphylococcus aureus (S. aureus) (Gram positive) and Escherichia coli (E. coli) (Gram negative) bacteria's were taken to study about antibacterial activity against the green synthesized Nickel oxide nanoparticles (NiO NPs). The Nickel oxide nanoparticles have pharmaceutical and other biomedical applications.

The use of tetraammonium tetrakis(4-sulphonato)phenyl porphyrin (TPPS), a water-soluble anionic compound, as a stain to analyse bacterial cells using fluorescent microscopy was investigated. TPPS was effectively used to analyse two different bacteria: Pseudomonas aeruginosa and Bacillus cereus. The variation in brightness with varying concentrations of TPPS was studied. The patterns of variations for these bacteria were found to be the same, but with consistently higher brightness for Bacillus cereus.

The photocatalytic activity of green synthesized nickel oxide nanoparticles derived using Sterculia foetida leaf extract was examined by introducing into graphene oxide (GO). Graphene oxide has porous nature which might enlarge the photocatalytic execution of NiO nanoparticles. NiO nanoparticles (NiO NPs) were prepared by solution combustion method and introduced into graphene oxide (GO) synthesized by Hummers method to form NiO@GO nanocomposite. Various techniques were used for the material characterization of the above synthesized compounds such as UV, FTIR, Powder X-ray diffraction (PXRD), Transmission electron microscopy (TEM). The photocatalytic execution of NiO@GO nanocomposite on deterioration of Methylene blue (MB) and Malachite Green (MG) have been examined and the results indicate that the GO plays a critical part in the improvement of photocatalytic execution. Antibacterial activity has been carried out against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). The synthesized NiO@GO nanocomposite accomplishes a most extreme decay efficiency of 98% for Methylene blue than Malachite green under UV-light.