In-house validation of AF4-MALS-UV for polystyrene nanoplastic analysis

Battistini, Beatrice; Petrucci, Francesco; Bocca, Beatrice

doi:10.1007/s00216-021-03238-2

Cited by 16 publications

(6 citation statements)

References 58 publications

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“…Repeatability was studied for the D geo and t r for MALS and UV detectors, respectively. The results (Table 2 , top) confirmed that both detectors showed a repeatability within range of acceptance (RSD = 1–20%) [ 33 , 35 ]. A noticeable tendency is the increasing precision in the MALS detector, with the increase of the standard size, agreeing with the increasing sensibility of the equipment for higher sizes.…”

Section: Resultsmentioning

confidence: 60%

“…Although there are publications that explore the use of the AF4 to obtain size information of NPLs [ 32 , 33 ], there is still a lack of validated methodologies to apply in this field [ 11 , 24 ], also for the validation of standards in aqueous solution. Reference materials are being developed and validated methodologies; alternatives to SEM and TEM are needed for their measurement.…”

Section: Introductionmentioning

confidence: 99%

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Validated method for polystyrene nanoplastic separation in aqueous matrices by asymmetric-flow field flow fraction coupled to MALS and UV–Vis detectors

et al. 2023

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Plastics with nanosize (nanoplastics, NPLs) must be characterized, since they can be toxic or act as carriers of organic and inorganic pollutants, but there is a lack of reference materials and validated methods in the nanosize range. Therefore, this study has focused on the development and validation of a separation and size characterization methodology of polystyrene latex nanospheres, by using an asymmetric-flow field flow fraction system coupled to multi-angle light scattering and ultraviolet–visible detectors (AF4-MALS-UV). Hence, this work presents a fully validated methodology in the particle size range 30 to 490 nm, with bias between 95 and 109%, precision between 1 and 18%, LOD and LOQ below 0.2 and 0.3 µg respectively, except for 30-nm standard, for both detectors, and showing stable results for 100 analyses. Graphical Abstract

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Section: Resultsmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Validated method for polystyrene nanoplastic separation in aqueous matrices by asymmetric-flow field flow fraction coupled to MALS and UV–Vis detectors

et al. 2023

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“…As regards AF4-MALS, Battistini et al successfully validated an AF4-MALS method for the identication and quantication of nanosized PS particles (20-200 nm) at a LOD of 15-33 mg mL À1 . 94 The same accounts for the protocol of Bocca et al that was also able to quantify PS NPs (20-200 nm) at a LOD of 50 mg mL À1 . 101 However, both methods are limited to nanoplastics that present UV absorbance.…”

Section: Critical Reviewmentioning

confidence: 86%

Methodologies to characterize, identify and quantify nano- and sub-micron sized plastics in relevant media for human exposure: a critical review

Bruin

Rijke

Wezel

et al. 2022

Environ. Sci.: Adv.

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“…The morphology of an environmental micro/nanoplastic (MNPs) particle can be visually characterized by microscopy methods such as scanning electron microscopy–energy dispersive X-ray (SEM-EDX), transmission electron microscopy (TEM), atomic force microscopy (AFM), and stimulated emission depletion (STED) microscopy. To determine the size of MNPs, there are scattering methods such as dynamic light scattering (DLS), asymmetric flow field-flow fractionation with multi-angle light scattering or total organic carbon (AF 4 -MALS or AF 4 - TOC), and nanoparticle tracking analysis (NTA) . Spectroscopy methods used for chemical identification of MNPs include surface enhanced Raman (SERS), , Raman tweezers, , Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), and photoinduced forced microscopy (PiFM) .…”

Section: Introductionmentioning

confidence: 99%

Size-Resolved Identification and Quantification of Micro/Nanoplastics in Indoor Air Using Pyrolysis Gas Chromatography–Ion Mobility Mass Spectrometry

Hashemihedeshi,

Haywood,

Gatch

et al. 2024

J. Am. Soc. Mass Spectrom.

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Humans are exposed to differing levels of micro/nanoplastics (MNPs) through inhalation, but few studies have attempted to measure <1 μm MNPs in air, in part due to a paucity of analytical methods. We developed an approach to identify and quantify MNPs in indoor air using a novel pyrolysis gas chromatographic cyclic ion mobility mass spectrometer (pyr-GCxcIMS). Four common plastic types were targeted for identification, namely, (polystyrene (PS), polyethylene (PE), polypropylene (PP), and polymethyl methacrylate (PMMA). The method was applied to size-resolved particulate (56 nm to 18 μm) collected from two different indoor environments using a Micro-Orifice Uniform Deposit Impactors (MOUDI) model 110 cascade impactor. Comprehensive two-dimensional separation by GCxcIMS also enabled the retrospective analysis of other polymers and plastic additives. The mean concentrations of MNP particles with diameters of <10 μm and <2.5 μm in the laboratory were estimated to be 47 ± 5 and 27 ± 4 μg/m 3 , respectively. In the private residence, the estimated concentrations were 24 ± 3 and 16 ± 2 μg/m 3 . PS was the most abundant MNP type in both locations. Nontargeted screening revealed the presence of plastic additives, such as TDCPP (tris(1,3-dichloro-2-propyl)phosphate) whose abundance correlated with that of polyurethane (PU). This is consistent with their use as flame retardants in PU-based upholstered furniture and building insulation. This study provides evidence of indoor exposure to MNPs and underlines the need for further study of this route of exposure to MNPs and the plastic additives carried with them.

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In-house validation of AF4-MALS-UV for polystyrene nanoplastic analysis

Cited by 16 publications

References 58 publications

Validated method for polystyrene nanoplastic separation in aqueous matrices by asymmetric-flow field flow fraction coupled to MALS and UV–Vis detectors

Validated method for polystyrene nanoplastic separation in aqueous matrices by asymmetric-flow field flow fraction coupled to MALS and UV–Vis detectors

Methodologies to characterize, identify and quantify nano- and sub-micron sized plastics in relevant media for human exposure: a critical review

Size-Resolved Identification and Quantification of Micro/Nanoplastics in Indoor Air Using Pyrolysis Gas Chromatography–Ion Mobility Mass Spectrometry

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