In‐line coupling of supported liquid membrane extraction across nanofibrous membrane to capillary electrophoresis for analysis of basic drugs from undiluted body fluids

Abstract

Planar polyamide 6 nanofibrous membrane was for the first time used in direct coupling of supported liquid membrane (SLM) extraction to CE analysis. Disposable microextraction device with the nanofibrous membrane was preassembled and stored for immediate use. The membrane in the device was impregnated with 1 µL of 1‐ethyl‐2‐nitrobenzene and the device was subsequently filled with 10 µL of acceptor solution (10 mM HCl) and 15 µL of donor solution (sample). The device was in‐line coupled to CE system for selective extraction and direct injection, separation and quantification of model basic drugs (nortriptyline, haloperidol, loperamide and papaverine) from standard saline solutions (150 mM NaCl) and from undiluted human body fluids (urine and blood plasma). Compared to standard polypropylene supporting material, the nanofibrous membrane demonstrated superior characteristics in terms of lower consumption of organic solvents, constant volumes of operational solutions, full transparency and possibility to preassemble the devices. Extraction parameters were better or comparable for the nanofibrous vs. the polypropylene membrane and the hyphenated SLM‐CE method with the nanofibrous membrane was characterized by good repeatability (RSD ≤ 11.3%), linearity (r2 ≥ 0.9953; 0.5–20 mg/L), sensitivity (LOD ≤ 0.4 mg/L) and transfer (27–126%) of the basic drugs.

Applications of capillary electrophoresis in the fields of environmental, pharmaceutical, clinical, and food analysis (2019–2021)

“…The F I G U R E 5 Schematic diagram of magnetic SPE-CE (MSPE-CE) for the analysis of the atenolol enantiomers in plasma samples. Reproduced [47] with permission from Elsevier disposable pretreatment system coupled to CE-UV system for the quantitative analysis of the model essential drugs (norpine, droperidine, loperamide and papain) in undiluted body fluid at 200 nm has been illustrated [44]. In comparison with the traditional polypropylene SLMs, the advantages of polyamide 6 membrane included larger surface area, lower consumption and better extraction capacity.…”

“…A disposable pretreatment device using polyamide 6 nanofiber membrane as supporting liquid membrane (SLM) has been constructed for the first time, in which SLM was impregnated with 1 μL of 1‐ethyl‐2‐nitrobenzene and the device was subsequently filled with 10 μL of acceptor solution (10 mM HCl) and 15 μL of donor solution (sample). The disposable pretreatment system coupled to CE‐UV system for the quantitative analysis of the model essential drugs (norpine, droperidine, loperamide and papain) in undiluted body fluid at 200 nm has been illustrated [44]. In comparison with the traditional polypropylene SLMs, the advantages of polyamide 6 membrane included larger surface area, lower consumption and better extraction capacity.…”