In-ovo Antiviral Effect of Nigella sativa Extract against Newcastle Disease Virus in Experimentally Infected Chicken Embryonated Eggs

Abstract

Black seed (Nigella sativa) is an important medicinal herbal plant used to treat different ailments. In the present experiment antiviral effects of black seed was evaluated. Ethanolic extact of black seed was usd in the embryonated eggs inoculated with Newcastle Disease Virus (NDV). For this purpose Sixty (n=60) embryonated chicken eggs (14 th day), were randomly divided into 5 groups (G 1-5) with 12 eggs in each group. The treatment groups were G1 (control negative) and G2 (control positive) groups were inoculated with NDV @ 0.2ml/egg of (10-10.2 EID50), G3 (NDV+ Nigella sativa, 0.2mg /0.2ml), G4 (Nigella sativa only, 0.2mg/0.2 ml) and G5 (NDV+ Ribavirin 20µg/ml). Egg candling, histopathology and gross pathology were performed after 24, 48, 72 and 96 hours post inoculation. Different survival rates were observed in different groups such as, G1 and G4 (100%), G2 (33%), G3 (75%) and G5 (83%), respectively exhibiting significant difference (P<0.05) between groups. The gross study of G2 showed degeneration of thigh muscle and severe haemorrhages, while in G3 and G5 the lesions were not intense and showing viral inhibition effects of herbal extract and Ribavirin. Degeneration in hepatocytes, mild necrosis, lymphocytosis and increased number of macrophages were also evident in G3 and G5. There were dominant population of plasma cells, lymphocytes with mild degeneration and lymphoblasts. In G2 degeneration of bursal follicles, while in G3 and G5 aggregation of lymphocytes and lymphoblasts, developed bursal follicles with clear cortex and medulla was noticed. The gross examination and histopathology of the embryonated eggs revealed that Nigella sativa extract have strong immunotherapeutic effect against NDV infcetion.

Prophetic Medicine-Nigella Sativa (Black cumin seeds) – Potential herb for COVID-19?

“…The in vitro and in vivo studies of N.sativa has also shown antiviral efficacy against some other viruses like Murine cytomegalovirus (MCMV) [ 22 ], Papaya Ring Spot Virus [ 23 ], Hepatitis C Virus (HCV) [ 24 ], Avian influenza (H9N2) [ 25 ], Newcastle disease virus (NDV) [ 26 ] and Peste des Petits Ruminants (PPR) Virus [ 27 ]. It has been proposed that antiviral efficacy of N.sativa might be due to raised serum levels of interferon-gamma, increased CD4 count, augmented suppressor function and enhanced numbers of macrophages [ 22 ].…”

Fight against Covid-19: Nigella sativa, a Potential Curative

“…The in vitro and in vivo studies of N. Sativa have demonstrated antiviral efficacy against other viruses like Avian influenza (H9N2) [24], Murine cytomegalovirus (MCMV) [17], and Newcastle disease virus (NDV) [25].…”

Elucidating antiviral activity of Curcuma longa against H9 N2 influenza virus using embryonated chicken egg model

“…Different lymphatic tissues (spleen, bursa, Liver) were collected from embryos. A thin tissue section was stained with hematoxylin-eosin dye using histological paraffin embedding technique as suggested by (Khan et al, 2018).…”