2016
DOI: 10.1021/acs.analchem.6b01868
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In Ovo Sexing of Domestic Chicken Eggs by Raman Spectroscopy

Abstract: Male birds of egg-laying hen strains have no commercial value and are culled immediately after hatching, raising concerns for animal welfare. Existing experimental methods for in ovo sexing require taking samples and are applicable after embryos' sexual differentiation. We demonstrate that Raman spectroscopy enables contactless in ovo sex determination of the domestic chicken (Gallus gallus f. dom.) already at day 3.5 of egg incubation. A sexing accuracy of 90% was obtained by analyzing the spectra of blood ci… Show more

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Cited by 50 publications
(39 citation statements)
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“…Thermal damage of erythrocytes is avoided, as they move with high speed driven by the heart and remain in the laser focus for a fraction of second. [6] …”
Section: In Ovo Sexing By Spectroscopy Of Embryonic Bloodmentioning
confidence: 99%
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“…Thermal damage of erythrocytes is avoided, as they move with high speed driven by the heart and remain in the laser focus for a fraction of second. [6] …”
Section: In Ovo Sexing By Spectroscopy Of Embryonic Bloodmentioning
confidence: 99%
“…[6] The spectrum of embryonic blood is complex and most of the bands originate from haemoglobin. After baseline subtraction to remove the fluorescence background and normalization, the mean spectra of male and female eggs appear very similar.…”
Section: Raman Spectroscopymentioning
confidence: 99%
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“…Sex determination in ovo with near-infrared Raman spectroscopy enables contactless differentiation at day 4 of egg incubation via analysis of circulation blood spectra. According to Galli et al [ 7 ] the method is accompanied with lower hatching rates, requires egg-windowing, and the sexing is only 90% accurate; therefore a higher amount of eggs would have to be incubated. However, technique improvements could increase the hatching rate and accuracy [ 7 ].…”
Section: Introductionmentioning
confidence: 99%
“…Используемые на практике методы определения пола при сортировке однодневных цыплят немногочисленны [4]. Один из основных способов включает оценку формы рудиментарных бугорков в слизистой оболочке клоаки цыпленка.…”
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