In pursuit of enigmatic platelet antibodies ‐ anti‐HPA‐2b and anti‐HPA‐3a*

Minchinton, R. M.; Dawkins, B.; Chynoweth, L.; Pearson, Helen; Lown, J.A.G.; Shibata, Yoichi

doi:10.1111/j.1365-3148.1996.tb00081.x

Cited by 11 publications

(9 citation statements)

References 11 publications

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“…The MAIPA assay ( Kiefel et al ., 1987 ) is a sensitive technique which has led to considerable advances in platelet antibody detection, although certain antibodies have remained difficult to detect ( Minchinton et al ., 1996 ). The use of the PIFT together with the MAIPA assay is accepted by many laboratories as an optimal strategy for the investigation of platelet antibodies (19/22 international laboratories used either the PIFT and/or the MAIPA assay in the 9th ISBT Platelet Serology Workshop exercise in 1998).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of an enzyme‐linked immunosorbent assay kit (GTI PakPlus®) for the detection of antibodies against human platelet antigens

Lucas¹,

Rogers²

1999

Transfusion Medicine

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Twenty-six serum samples from 24 patients were investigated for the presence of platelet-specific antibodies in a partly retrospective (n = 15) and partly prospective (n = 9) study. The sera contained either alloantibodies to human platelet antigens (HPA) (n = 23) or were from clinically suspected cases of fetomaternal alloimmune thrombocytopenia (FMAITP) in which platelet-specific antibodies had not been detected (n = 3). Three techniques were used to detect platelet antibodies: the platelet immunofluorescence test, the monoclonal antibody immobilization of platelet antigens (MAIPA) assay and a commercially available enzyme-linked immunosorbent assay--GTI PakPlus (GTI kit). Two alkaline phosphatase-conjugated antiglobulin reagents provided by the manufacturer were used in the GTI kit: an antihuman IgG/IgA/IgM (IgGAM) conjugate and an antihuman IgG conjugate. The GTI kit with the anti-IgGAM conjugate failed to detect eight antibody specificities in seven sera (anti-HPA-1a [n = 3], anti-HPA-3a [n = 1], anti-HPA-3b [n = 1] and anti-HPA-5b [n = 3]). Greater signal-to-background ratios were achieved in the GTI kit with the anti-IgG conjugate but five antibody specificities (anti-HPA-1a [n = 1], anti-HPA-3a [n = 1], anti-HPA-3b [n = 1], anti-HPA-5b [n = 2]) remained undetectable. All the sera were detected by MAIPA assay and, furthermore, the MAIPA assay achieved the greatest signal-to-background ratio in the majority of sera tested. These findings re-emphasize the value of the MAIPA assay in reference laboratories and illustrate that the GTI kit may either fail to detect or incorrectly identify clinically significant HPA antibodies.

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Section: Discussionmentioning

confidence: 99%

Evaluation of an enzyme‐linked immunosorbent assay kit (GTI PakPlus®) for the detection of antibodies against human platelet antigens

Lucas¹,

Rogers²

1999

Transfusion Medicine

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“…However, IgG2 was never found. The higher frequency of IgG3-and IgG4-and the detection of IgG2 subclasses of the antibody in the present study is likely to be due to the greater sensitivity of the MAIPA assay compared to the immunofluorescence technique (14) and to the use of undiluted sera instead of diluted sera employed in the study by Proulx et al (13). In our investi-gation the similar anti-HPA-la IgG subclass composition of the severely thromocytopenic and mildly thrombocytopenichnaffected groups of sera is in keeping with the findings of Proulx et al (13).…”

Section: Discussionmentioning

confidence: 66%

Immunoglobulin G subclasses of anti‐human platelet antigen la in maternal sera: relation to the severity of neonatal alloimmune thrombocytopenia

Mawas

Wiener

Williamson

et al. 1997

European J of Haematology

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The monoclonal antibody immobilization of platelet antigen (MAIPA) technique was employed to detect and semiquantitatively assess total IgG anti‐HPA‐1a and its subclasses in sera of mothers who gave birth to severely thrombocytopenic (<50×109 platelets/L) (n = 14) or mildly thrombocytopenic/unaffected (>50×109 platelets/L) (n = 13) neonates. There was no statistically significant difference between the IgG anti‐HPA‐1a subclass composition of the 2 groups of sera. The majority of sera (26/27,96%) showed IgG1+IgG3 while 17/27 (63%) had all 4 subclasses of the antibody. No significant differences between the severely thrombocytopenic and mildly thrombocytopenic/unaffected groups were detected in the levels of IgG, IgG1, IgG2 or IgG4 of the antibody. However, the values of IgG3 anti‐HPA‐1a were significantly higher in the severely thrombocytopenic than in the mildly thrombocytopenic/unaffected group of sera with only little overlap (median 2.94 vs. 1.68, range 1.36–9.71 vs. 1.50–2.84, respectively; p <0.01). The results suggest that maternal IgG3 anti‐HPA‐1a has predictive value for severe thrombocytopenia of the neonate. However, a prospective study of IgG HPA‐1a subclasses in a greater number of maternal sera at different times of pregnancy is needed to test if IgG3 anti‐HPA‐1a is predictive of the degree of fetal/neonatal thrombocytopenia.

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“…Strong evidence for difficulties in the detection of HPA‐3 antibodies comes from various workshops on platelet immunobiology and from other reports [2,7,8]. As shown by Goldberger et al .…”

Section: Discussionmentioning

confidence: 97%

Delayed detectability of anti‐HPA‐3a by the MAIPA assay in a severe neonatal alloimmune thrombocytopenia, but successful transfusion of incompatible donor platelets: a case report

Schallmoser¹,

Kutschera

Macher³

et al. 2006

Vox Sanguinis

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In pursuit of enigmatic platelet antibodies ‐ anti‐HPA‐2b and anti‐HPA‐3a*

Cited by 11 publications

References 11 publications

Evaluation of an enzyme‐linked immunosorbent assay kit (GTI PakPlus®) for the detection of antibodies against human platelet antigens

Evaluation of an enzyme‐linked immunosorbent assay kit (GTI PakPlus®) for the detection of antibodies against human platelet antigens

Immunoglobulin G subclasses of anti‐human platelet antigen la in maternal sera: relation to the severity of neonatal alloimmune thrombocytopenia

Delayed detectability of anti‐HPA‐3a by the MAIPA assay in a severe neonatal alloimmune thrombocytopenia, but successful transfusion of incompatible donor platelets: a case report

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