In search of the visual pigment template

Govardovskii, V. I.; Fyhrquist, Nanna; Reuter, Tom; Kuzmin, Dmitry G.; Donner, Karl Johan

doi:10.1017/s0952523800174036

Cited by 959 publications

(1,121 citation statements)

References 62 publications

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“…Cells were harvested before isolating rhodopsin photopigments by affinity immunochromatography and binding to excess 11-cis retinal in the dark. 19,20 Dark and bleached absorbance spectra were recorded using a UV-visible spectrophotometer, and the peak sensitivity value, the lambda max (λ max ), was determined by fitting to a standard rhodopsin template 21 as previously described 19,20 (Supplementary Materials and Methods online).…”

Section: Construct Generationmentioning

confidence: 99%

Next-generation sequencing in health-care delivery: lessons from the functional analysis of rhodopsin

Davies

Downes

et al. 2012

Genetics in Medicine

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original research articlePurpose: The interpretation of genetic information has always been challenging, but next-generation sequencing produces data on such a vast scale that many more variants of uncertain pathogenicity will be found. We exemplify this issue with reference to human rhodopsin, in which pathogenic mutations can lead to autosomal dominant retinitis pigmentosa.methods: Rhodopsin variants, with unknown pathogenicity, were found in patients by next-generation and Sanger sequencing and a multidisciplinary approach was used to determine their functional significance.Results: Four variants in rhodopsin were identified: F45L, P53R, R69H, and M39R, with the latter two substitutions being novel. We investigated the cellular transport and photopigment function of all four human substitutions and found that the F45L and R69H variants behave like wild-type and are highly unlikely to be pathogenic. By contrast, P53R (a de novo change) and M39R were retained in the endoplasmic reticulum with significantly reduced functionality and are clearly pathogenic.conclusion: Potential pathogenicity of variants requires careful assessment using clinical, genetic, and functional data. We suggest that a multidisciplinary pathway of assessment, using several functional assays, will be required if next-generation sequencing is to be used effectively, reliably, and safely in the clinical environment. 2012:14(11):891-899 Genet Med

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Section: Construct Generationmentioning

confidence: 99%

Next-generation sequencing in health-care delivery: lessons from the functional analysis of rhodopsin

Davies

Downes

et al. 2012

Genetics in Medicine

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“…These measurements were made at four different flicker rates, ranging from 10 to 25 Hz. Each pair of the sensitivity values obtained from this procedure was best fitted using standard photopigment absorption curves (Govardovskii et al 2001) with the k max values determined to the nearest 0.1 nm. The logic of the experiment was that if the 510 nm peak value obtained earlier reflected mostly contributions from rods, then those signals should become progressively attenuated at higher flicker rates, i.e., one should see a Purkinje shift.…”

Section: Erg Measurementsmentioning

confidence: 99%

Visual pigments of marine carnivores: pinnipeds, polar bear, and sea otter

et al. 2006

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Rod and cone visual pigments of 11 marine carnivores were evaluated. Rod, middle/long-wavelength sensitive (M/L) cone, and short-wavelength sensitive (S) cone opsin (if present) sequences were obtained from retinal mRNA. Spectral sensitivity was inferred through evaluation of known spectral tuning residues. The rod pigments of all but one of the pinnipeds were similar to those of the sea otter, polar bear, and most other terrestrial carnivores with spectral peak sensitivities (k max ) of 499 or 501 nm. Similarly, the M/L cone pigments of the pinnipeds, polar bear, and otter had inferred k max of 545 to 560 nm. Only the rod opsin sequence of the elephant seal had sensitivity characteristic of adaptation for vision in the marine environment, with an inferred k max of 487 nm. No evidence of S cones was found for any of the pinnipeds. The polar bear and otter had S cones with inferred k max of $440 nm. Flickerphotometric ERG was additionally used to examine the in situ sensitivities of three species of pinniped. Despite the use of conditions previously shown to evoke cone responses in other mammals, no cone responses could be elicited from any of these pinnipeds. Rod photoreceptor responses for all three species were as predicted by the genetic data.

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“…The rhodopsin and metarhodopsin spectra used in the calculations were obtained with the visual pigment template of Govardovskii et al (2000), where spectral shape is fully determined by the wavelength of maximal absorption. Calculations with visual pigment spectra peaking at slightly different wavelengths demonstrated that the anomalous effects of Fig.…”

Section: Blue Visual Pigmentmentioning

confidence: 99%

Visual pigment spectra of the comma butterfly, Polygonia c-album, derived from in vivo epi-illumination microspectrophotometry

Vanhoutte

Stavenga

2005

J Comp Physiol A

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Visual pigment spectra of the comma butterfly, Polygonia c-album, derived from in vivo epiillumination nation microspectrophotometry Vanhoutte, KJA; Stavenga, DG Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Abstract The visual pigments in the compound eye of the comma butterfly, Polygonia c-album, were investigated in a specially designed epi-illumination microspectrophotometer. Absorption changes due to photochemical conversions of the visual pigments, or due to lightindependent visual pigment decay and regeneration, were studied by measuring the eye shine, i.e., the light reflected from the tapetum located in each ommatidium proximal to the visual pigment-bearing rhabdom. The obtained absorbance difference spectra demonstrated the dominant presence of a green visual pigment. The rhodopsin and its metarhodopsin have absorption peak wavelengths at 532 nm and 492 nm, respectively. The metarhodopsin is removed from the rhabdom with a time constant of 15 min and the rhodopsin is regenerated with a time constant of 59 min (room temperature). A UV rhodopsin with metarhodopsin absorbing maximally at 467 nm was revealed, and evidence for a blue rhodopsin was obtained indirectly.

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In search of the visual pigment template

Cited by 959 publications

References 62 publications

Next-generation sequencing in health-care delivery: lessons from the functional analysis of rhodopsin

Next-generation sequencing in health-care delivery: lessons from the functional analysis of rhodopsin

Visual pigments of marine carnivores: pinnipeds, polar bear, and sea otter

Visual pigment spectra of the comma butterfly, Polygonia c-album, derived from in vivo epi-illumination microspectrophotometry

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