Microbial respiratory reduction of nitrous oxide (N 2 O) to dinitrogen (N 2 ) via denitrification plays a key role within the global N-cycle since it is the most important process for converting reactive nitrogen back into inert molecular N 2 . However, due to methodological constraints, we still lack a comprehensive, quantitative understanding of denitrification rates and controlling factors across various ecosystems. We investigated N 2 , N 2 O and NO emissions from irrigated cotton fields within the Aral Sera Basin using the He/O 2 atmosphere gas flow soil core technique and an incubation assay. NH 4 NO 3 fertilizer, equivalent to 75 kg ha −1 and irrigation water, adjusting the water holding capacity to 70, 100 and 130% were applied to the incubation vessels to assess its influence on gaseous N emissions. Under soil conditions as they are naturally found after concomitant irrigation and fertilization, denitrification was the dominant process and N 2 the main end product of denitrification. The mean ratios of N 2 /N 2 O emissions increased with increasing soil moisture content. N 2 emissions exceeded N 2 O emissions by a factor of 5±2 at 70% soil water holding capacity (WHC) and a factor of 55±27 at 130% WHC. The mean ratios of N 2 O/NO emissions varied between 1.5±0.4 (70% WHC) and 644±108 (130% WHC). The magnitude of N 2 emissions for irrigated cotton was estimated to be in the range of 24±9 to 175±65 kg-N ha −1 season −1 , while emissions of NO were only of minor importance (between 0.1 to 0.7 kg-N ha −1 season −1 ). The findings demonstrate that for irrigated dryland soils in the Aral Sera Basin, denitrification is a major pathway of N-loss and that substantial amounts of Nfertilizer are lost as N 2 to the atmosphere for irrigated dryland soils.