In Silico Analysis of the Potential of the Active Compounds Fucoidan and Alginate Derived from Sargassum Sp. as Inhibitors of COX-1 and COX-2

Dewi, Lestari

doi:10.5455/medarh.2016.70.172-176

Cited by 6 publications

(3 citation statements)

References 26 publications

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“…Similarly, they reduced COX-2 mRNA expression by 82.1, 65.2, 64.1, and 70.2%, respectively, relative to the control group, in a similar manner to that of dexamethasone, which revealed a reduction of 85.2% at 40 μg/mL, where MIF1 revealed the highest inhibitory potential at all examined doses. This coincides with in silico studies of fucoidan-type sulfated polysaccharides, which turned out to be potent inhibitors of COX-1 and COX-2, and their effect may be greater than that of alginates [ 52 ]. Another study showed that Fucus vesiculosus fucoidan showed the better inhibition of both isoforms of the COX enzyme compared to indomethacin; the inhibition of COX-2 was higher than COX1 [ 53 ].…”

Section: Resultssupporting

confidence: 84%

FTIR Characterization of Sulfated Polysaccharides Obtained from Macrocystis integrifolia Algae and Verification of Their Antiangiogenic and Immunomodulatory Potency In Vitro and In Vivo

et al. 2022

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The aim of this study was to evaluate the antiangiogenic and immunomodulatory potential of sulfated polysaccharides from the marine algae Macrocystis integrifolia characterized by FTIR. The cytotoxicity of sulfated polysaccharides was evaluated using the 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay. Antiangiogenic activity was evaluated using the chicken chorioallantoic membrane (CAM) assay. Immunomodulatory activity was determined on macrophage functionality and allergic response. The results showed that sulfated polysaccharides significantly decreased angiogenesis in chicken chorioallantoic membranes (p < 0.05). Likewise, they inhibited in vivo chemotaxis and in vitro phagocytosis, the transcription process of genes that code the enzymes cyclooxygenase−1 (COX−1), cyclooxygenase-2 (COX-2) and nitric oxide synthase-2 (NOS-2) and the nuclear factor kappa-light chain enhancer of activated B cells (NF-κB), showing immunomodulatory properties on the allergic response, as well as an in vivo inhibitory effect in the ovalbumin-induced inflammatory allergy model (OVA) and inhibited lymphocyte proliferation specific to the OVA antigen in immunized mice. Finally, these compounds inhibited the histamine-induced skin reaction in rats, the production of immunoglobulin E (IgE) in mice, and the passive response to skin anaphylaxis in rats. Therefore, the results of this research showed the potential of these compounds to be a promising source for the development of antiangiogenic and immunomodulatory drugs.

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Section: Resultssupporting

confidence: 84%

FTIR Characterization of Sulfated Polysaccharides Obtained from Macrocystis integrifolia Algae and Verification of Their Antiangiogenic and Immunomodulatory Potency In Vitro and In Vivo

et al. 2022

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“…berupa fukoidan dan alginat berpotensi menghambat enzim cyclooxygenase 1 dan 2 dalam proses peradangan. Sehingga, hal ini dapat memberikan efek yang menguntungkan terhadap proses peradangan pada kondisi patologis (Dewi, 2016). Hal ini didukung oleh hasil studi Oka dkk, bahwa kadungan senyawa bioaktif pada Sargassum sp yakni fukoidan yang merupakan polisakarida tersulfasi pada rumput laut memiliki aktivitas antimikroba kuat dalam melawan Candida albicans, S. mutans, dan P. gingivalis serta mampu menetralkan endotoksin (lipopolisakarida) dalam uji LAL (Oka et al, 2019).…”

Section: Journal Of Holistic Nursing and Health Scienceunclassified

Dampak dan Terapi Non-Farmakologis Periodontitis pada Ibu Hamil; Kajian Literatur

Apriani¹,

Sunarjo

Widyawati

et al. 2022

Holistic Nursing and Health Science

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Introduction: According to WHO, periodontitis is a disease that affects about 20-50% of the world’s population. Periodontitis in pregnancy is often associated with poor pregnancy outcomes. Nowadays, periodontitis therapy is still giving antibiotics and mouth rinses (chlorhexidine) to reduce the dental plaque. However, these therapies have side effects in the form of antibiotic resistance, allergies and mouth irritation. Therefore, a non-conventional therapeutic that is more effective and safe is needed. Brown seaweed (Sargassum sp) is a promising alternative to its natural potential compounds as periodontitis therapy. The purpose of this study was to analyze the effect of Sargassum sp and the impact of periodontitis on pregnancy. Methods: This research is a systematic literature review by searching literature through various databases on; PubMed, Cochrane, ScienceDirect, and Google Scholar. The literature was examined in the span of 2015 to 2020. The criteria and keywords were; Sargassum and Periodontitis; Periodontitis and Pregnancy; Periodontal Disease and Pregnancy. Results: The results of this review found 12 articles that showed that periodontitis is one of the risk factors in pregnancy such as; preeclampsia, LBW, and premature. Furthermore, 9 other articles discussed the antibacterial and anti-inflammatory activity contained in Sargassum sp, which can inhibit the development of P.gingivalis and its virulence factors. Conclusion: The conclusion is metabolites of Sargassum sp “alginate” were able overcomed periodontitis in pregnancy. Thus, further research is expected to be able to intervene directly and analyze more broadly about the effect of Sargassum sp on periodontitis in pregnancy.

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“…The COX is an enzyme that mediates inflammation responses by prostaglandins (Dewi, 2016). The COX-2 enzyme is a significant isoenzyme in the inflammatory process but the data showed that prostanoids produced by COX-1 also play a role in inflammation (Smith & Langenbach, 2001).…”

mentioning

confidence: 99%

Virtual prediction of Brown Rice Gamma Oryzanol as anti-inflammation via COX-1 and COX-2 inhibitions

Palis,

Safitri,

Wijayanti

et al. 2023

Berkala Penelitian Hayati

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Brown rice gamma oryzanol (GO) is an important bioactive compound with numerous potential health functions, such as anti-inflammatory and anti-diabetic. Cyclooxygenase (COX) is the enzyme that mediates the biological transformation of arachidonic acid (ARA) into prostaglandins (PGs) in the mechanism of inflammation. This study purposed to identify the potency of gamma oryzanol of brown rice as an anti-inflammatory compound via COX-1 and COX-2 inhibition. The three-dimensional structure of COX-1 and COX-2 was determined by binding affinity calculations from virtual molecular docking with gamma oryzanol ligand through in silico study. Arachidonic acid as native ligand of COX-1/COX-2 was performed as a control in this study. Gamma oryzanol (CIS 5282164) and arachidonic acid (CIS 444899) were downloaded in 3D-SDF format from the PubChem database. The three-dimensional model of COX-1 and COX-2 was acquired from the protein data bank (www.rscb.org) in PDB format. The molecular docking was performed by Vina Wizard integrated on PyRx version 0.8. The interaction between GO and COX-1/COX-2 was visualized using the Discovery Studio version 2022 software. Molecular dynamics (MD) simulation also used to analyze the stability of the protein-ligand complex. The results showed that brown rice gamma oryzanol binds to COX-1 and COX-2 at many active sites with strongest binding affinity compared to arachidonic acid, with energy binding -7.8 kcal/mol and -6.2 kcal/mol, respectively. This in silico study concluded that brown rice gamma oryzanol has potential function as an anti-inflammatory activity through COX-1 and COX-2 catalysis reduction.

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In Silico Analysis of the Potential of the Active Compounds Fucoidan and Alginate Derived from Sargassum Sp. as Inhibitors of COX-1 and COX-2

Cited by 6 publications

References 26 publications

FTIR Characterization of Sulfated Polysaccharides Obtained from Macrocystis integrifolia Algae and Verification of Their Antiangiogenic and Immunomodulatory Potency In Vitro and In Vivo

FTIR Characterization of Sulfated Polysaccharides Obtained from Macrocystis integrifolia Algae and Verification of Their Antiangiogenic and Immunomodulatory Potency In Vitro and In Vivo

Dampak dan Terapi Non-Farmakologis Periodontitis pada Ibu Hamil; Kajian Literatur

Virtual prediction of Brown Rice Gamma Oryzanol as anti-inflammation via COX-1 and COX-2 inhibitions

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