In silico analysis of virulence genes in an emerging dental pathogen A. baumannii and related species

Priyadharsini, J. Vijayashree; Girija, A. S. Smiline; Paramasivam, Arumugam

doi:10.1016/j.archoralbio.2018.07.001

Cited by 71 publications

(42 citation statements)

References 22 publications

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“…The ability to form biofilm and resist desiccation is a key property of A. baumannii which makes it refractory to endodontic and periodontal treatments. The biofilms produced by the bacteria are also potential reservoirs of pathogens associated with pneumonia and chronic obstructive pulmonary disease [9, 10]. In a polymicrobial community, A. baumannii protects carbapenems-susceptible bacteria, thus aggravating the disease process during treatment with carbapenems [11].…”

Section: Introductionmentioning

confidence: 99%

An insight into the emergence of Acinetobacter baumannii as an oro-dental pathogen and its drug resistance gene profile – An in silico approach

Priyadharsini

Girija

Paramasivam

2018

Heliyon

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BackgroundAcinetobacter baumannii, a potential nosocomial pathogen has stealthily gained entry into the oral cavity. Their association with other pathogens like Pseudomonas aeruginosa in chronic and aggressive periodontitis cases is well documented. The magnitude of problem caused by A.baumannii could be attributed to resistance genes acquired by the organism. Since the microbiome of oral cavity is heterogeneous and complex, the transfer of genes from multidrug resistant A.baumannii may be a serious threat in infection control and management. In view of this fact, the present study aims to categorize and characterize drug resistant genes present in each of the 19 genomes of Acinetobacter Sp. selected for the study.MethodsAbout 19 genome sequences of Acinetobacter spp. with the predominance of different strains of A.baumannii was genotyped using in silico restriction digestion and pulse field gel electrophoresis (PFGE). Further, the prevalence of common drug resistant genes in the genome of various Acinetobacter spp. was recorded using in silico PCR analysis.ResultsBased on the PFGE pattern, phylogenetic tree was constructed and the genomes were clustered into 6 genotypes. Genotype 4 (n = 8; 42.10%) and 5 (n = 6; 31.57%) were predominant, followed by genotypes 2 (n = 2; 10.52%), 1, 3 and 6 (n = 1; 5.26%). Three species were excluded from the list since they were negative for most of the drug resistant genes tested. Prevalence of drug resistant genes in each of the 16 genomes analysed found oxa-51, ISAba 1 and ADC 1 to be the major genes found in A.baumannii. Acinetobacter spp. belonging to genotypes 4 and 5 were found to harbour 6–10 and 2–8 potential drug resistant genes respectively.ConclusionThe present study showed cluster of multi-drug resistant genes in genomes analysed, thus, warranting the need for antibiotic surveillance, alternate therapeutic measures and development of novel antimicrobials. An extensive study on the genes conferring drug resistance in this pathogen will open new avenues for battling the entry and spread of this pathogen in vulnerable patient groups.

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Section: Introductionmentioning

confidence: 99%

An insight into the emergence of Acinetobacter baumannii as an oro-dental pathogen and its drug resistance gene profile – An in silico approach

Priyadharsini

Girija

Paramasivam

2018

Heliyon

Self Cite

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“…81.7% people think that viruses can be transmitted from person to person very easily by currency notes(Fig- 9), which is very true, confederation of all India traders(CAIT) stated that currency notes are most vulnerable to health of people and are easy carriers of communicable diseases. 75.2% of the respondents thinks that viral born infections like COVID-19 can transmit through aerosols(Fig- 10), which is true, WHO stated that,In the context of COVID-19, airborne transmission may be possible in specific circumstances and settings in which procedures or support treatments that generate aerosols are performed The initial outbreak of coronavirus is in China in December 2019 the COVID-19 disease has had a cascading effect worldwide(Vijayashree Priyadharsini, Smiline Girija and Paramasivam, 2018a; Inserm and INSERM, 2020).According to the ICMR update on March 23, 2020 more than 400 individuals have been tested COVID-19 positive in India (Priyadharsini et al, 2018).The identification and isolation of a suspected case is the most important step in curing the spread of COVID-19. However, in our study, less than half of the respondents were aware of defining a close contact according to USCDC A close contact is defined as being within approximately 6 feet(2meters) for a prolonged period of time for having direct contact with infectious a creation of a COVID-19 case(Sohaib Shahzan, Smiline Girija and Vijayashree Priyadharsini, 2019).…”

Section: Resultsmentioning

confidence: 99%

Awareness About Viral Infections and Its Prevention Among Public After Covid-19 Pandemic

2021

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Information on awareness of public towards viral infections, it's coverage in general public, prevention of viral infection is like Covid-19 is the major challenge for every country and it formed the basis of healthcare forces. Therefore, it is important to assess the attitude and awareness towards viral infections after Covid-19 pandemic, for the further health policies implementation as well as taking safety measures and following the guidelines. The study objective was to assess the awareness about viral infections and its prevention among the public after Covid-19 pandemic, from the various places of the country. Study was carried out by using multiple-choice structured questionnaires among 119 attendees. The questionnaire was prepared in such a way as to assess the knowledge of the public towards the infection, transmission, screening and prevention of viral infections. The data was collected and it analyses statistically.Although most of the attendants were aware of the existence of COVID-19 and the many other viral infections, the level of awareness regarding prevention, transmission and following the safety guidelines was found to be dissatisfactory. Direct correlation was found between educational level and awareness, which reversed the fact that the attendants with better knowledge and information had better attitudes towards the infection and prevention of COVID-19. Within the limitations of the study, the moderate level of awareness about viral infections and prevention after COVID-19 pandemic is observed

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“…This episode was first distinguished in Foshan, Guangdong, China on november 2002. SARS -COV is believed to be a creature infection (Priyadharsini et al, 2018b)from a questionable creature supply, maybe bats that spread to different creatures and first contaminated people in china. Transmission was fundamentally from individual to person (Lal, 2010).…”

Section: Sarsmentioning

confidence: 99%

Viral Pandemics and Its Effect on General Public - A Review

2021

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The pandemic is an outbreak of disease which spreads across a particular region or regions. The key features of pandemic include wide geographic extension, disease movement, novelty, severity, high attack rates and explosiveness. It also involves minimal population immunity, infectiousness and contagiousness which helps us to understand what pandemics are and its effect on the public. Viral pathogens can transform quickly, which allows them to pass from wild animals to humans. These emerging diseases endanger human lives and have major socioeconomic impacts. The aim of the review is to know about the viral pandemics and its effect on the general public. Human behaviour and demographic factors significantly increase these risks, and the speed with which humans travel between continents can cause the runaway spread of pandemics. The literature review was carried from scopus and pubmed databases from 2000 till date. The viral pandemic disease and its effect on the general public is the outbreak of global scale. Children, older people, pregnant women and people with weakened immune systems are often affected by these pandemics. The cause, prevention and control of pandemics had been studied.

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In silico analysis of virulence genes in an emerging dental pathogen A. baumannii and related species

Cited by 71 publications

References 22 publications

An insight into the emergence of Acinetobacter baumannii as an oro-dental pathogen and its drug resistance gene profile – An in silico approach

An insight into the emergence of Acinetobacter baumannii as an oro-dental pathogen and its drug resistance gene profile – An in silico approach

Awareness About Viral Infections and Its Prevention Among Public After Covid-19 Pandemic

Viral Pandemics and Its Effect on General Public - A Review

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