In-silico and in-vitro analysis of endocan interaction with statins

Kumar, Sarwareddy Kartik; Sureshan, Muthusamy; Saraboji, K.; Mani, Krishna Priya

doi:10.1016/j.ijbiomac.2019.09.235

Cited by 13 publications

(7 citation statements)

References 57 publications

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“…About 1 × 10 4 Int 407 cells/well were seeded in a 96-well microtiter plate and incubated at 37 °C in a CO 2 incubator for 24 h. After the incubation period, the cells were treated with various concentrations (100 μg/mL, 50 μg/mL, 25 μg/mL, 12.5 μg/mL, 6.25 μg/mL) of CAP-2M4VP and incubated for another 24 h at 37 °C in a CO 2 incubator. After 24 h, 1 mg/mL of MTT was added, and the microtiter plate was incubated at 37 °C for 3 h. Dimethyl sulfoxide (DMSO) was added to dissolve the formazan crystals, and absorbance was read at 570 nm using a microplate reader spectrophotometer (Synergy H1) 20 .…”

Section: Methodsmentioning

confidence: 99%

Development of a smart pH-responsive nano-polymer drug, 2-methoxy-4-vinylphenol conjugate against the intestinal pathogen, Vibrio cholerae

Sarveswari

Gupta

Durai

et al. 2023

Sci Rep

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Vibrio cholerae causes cholera, an acute diarrhoeal disease. The virulence in V. cholerae is regulated by the quorum-sensing mechanism and response regulator LuxO positively regulates the expression of virulence determinants adhesion, biofilm formation, and cholera toxin production. Previous in-silico studies revealed that 2-methoxy-4-vinylphenol could bind to the ATP binding site of LuxO and the complex was compact and stable in pHs like intestinal pHs. Here, we have explored the polymeric nano-formulation of 2-methoxy-4-vinylphenol using cellulose acetate phthalate for controlled drug release and their effectiveness in attenuating the expression of V. cholerae virulence. Physico-chemical characterization of the formulation showed particles with a mean size of 91.8 ± 14 nm diameter and surface charge of − 14.7 ± 0.07 mV. The uniform round polymeric nanoparticles formed displayed about 51% burst release of the drug at pH 7 by 3rd h, followed by a controlled linear release in alkaline pH. The polymeric nanoparticles demonstrated a tenfold increase in intestinal membrane permeability ex-vivo. At lower concentrations, the 2-methoxy-4-vinylphenol polymeric nanoparticles were non-cytotoxic to Int 407 cells. In-vitro analysis at pH 6, pH 7, pH 8, and pH 9 revealed that cellulose acetate phthalate—2-methoxy-4-vinylphenol nanoparticles were non-bactericidal at concentrations up to 500 μg/mL. At 31.25 μg/mL, the nanoparticles inhibited about 50% of the biofilm formation of V. cholerae MTCC 3905 and HYR14 strains. At this concentration, the adherence of V. cholerae MTCC 3905 and HYR14 to Int 407 cell lines were also significantly affected. Gene expression analysis revealed that the expression of tcp, qrr, and ct at pH 6, 7, 8, and 9 has reduced. The CAP-2M4VP nanoparticles have demonstrated the potential to effectively reduce the virulence of V. cholerae in-vitro.

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Section: Methodsmentioning

confidence: 99%

Development of a smart pH-responsive nano-polymer drug, 2-methoxy-4-vinylphenol conjugate against the intestinal pathogen, Vibrio cholerae

Sarveswari

Gupta

Durai

et al. 2023

Sci Rep

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“…The expression of endocan increases under the stimulation of IL-1β, TNF-α and other pro-inflammatory factors, which further increases the levels of VCAM-1 and ICAM-1, thereby enhancing the adhesion between leukocytes and endothelial cells, and promoting the recruitment and migration of inflammatory cells. In addition, endocan treatment can stimulate the production of NO and ROS, and increase the expression of iNOS and CRP in RAW264.7 macrophages ( 5 ) ( Figure 3 ). These studies prove that endocan can accelerate endothelial cell dysfunction by promoting inflammation, cell adhesion and oxidative stress.…”

Section: Endocan and The Cardiovascular Systemmentioning

confidence: 99%

“…Impaired endothelial cell integrity and function play an important role in atherosclerosis and ischemic heart. Damage to the endothelium leads to an increase in the production of reactive oxygen species (ROS), which can reduce the production of NO by increasing the concentration of calcium ions in the cytoplasm ( 4 , 5 ). The increased production of ROS and the impairment of NO availability can induce and maintain the inflammatory state of the blood vessel wall.…”

Section: Introductionmentioning

confidence: 99%

Endocan: A Key Player of Cardiovascular Disease

Chen

Jiang

et al. 2022

Front. Cardiovasc. Med.

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Endothelial dysfunction is considered to be an early change in atherosclerosis. Endocan, also known as endothelial cell specific molecule-1, is a soluble proteoglycan mainly secreted by endothelial cells. Inflammatory factors such as IL-1β and TNF-α can up regulate the expression of endocan and then affect the expression of cell adhesion molecules, such as ICAM-1 and VCAM-1, which play an important role in promoting leukocyte migration and inflammatory response. Elevated plasma levels of endocan may reflect endothelial activation and dysfunction, and is considered to be a potential immuno-inflammatory marker that may be related to cardiovascular disease. In the case of hypertension, diabetes, angina pectoris and acute myocardial infarction, the increase or decrease of serum endocan levels is of great significance. Here, we reviewed the current research on endocan, and emphasis its possible clinical value as a prognostic marker of cardiovascular disease. Endocan may be a useful biomarker for the prognosis of cardiovascular disease, but more research is needed on its mechanism of action.

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“…Endocan could induce vascular inflammation via macrophage activation [46]. In turn, 5 and 10 mmol/l concentrations of simvastatin exerted antagonist activity on endocan expression in endothelial cells and suppressed this effect [46]. Endocan, chemerin and galectin-3 are discrete biomarkers associated with CVD [47].…”

Section: Effect Of Medications On Endocan Levelsmentioning

confidence: 99%

Endocan: a new marker of endothelial function

Balta

Mikhailidis

2021

Current Opinion in Cardiology

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Purpose of reviewTo consider the role of endocan as an inflammatory marker in cardiovascular diseases. Recent findingsEndocan, an endothelial inflammatory marker, is associated with cardiovascular disease. SummaryVascular endothelial inflammation plays a key role in the pathogenesis of inflammatory and cardiovascular diseases by influencing thrombogenesis, tumour invasion and secretion of bioactive mediators. We discuss the role of endocan mainly in the context of cardiology.

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In-silico and in-vitro analysis of endocan interaction with statins

Cited by 13 publications

References 57 publications

Development of a smart pH-responsive nano-polymer drug, 2-methoxy-4-vinylphenol conjugate against the intestinal pathogen, Vibrio cholerae

Development of a smart pH-responsive nano-polymer drug, 2-methoxy-4-vinylphenol conjugate against the intestinal pathogen, Vibrio cholerae

Endocan: A Key Player of Cardiovascular Disease

Endocan: a new marker of endothelial function

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