In Silico and In Vitro Analysis of Multifunctionality of Animal Food-Derived Peptides

Abstract

Currently, the associations between oxidative stress, inflammation, hypertension, and metabolic disturbances and non-communicable diseases are very well known. Since these risk factors show a preventable character, the searching of food peptides acting against them has become a promising strategy for the design and development of new multifunctional foods or nutraceuticals. In the present study, an integrated approach combining an in silico study and in vitro assays was used to confirm the multifunctionality of milk and meat protein-derived peptides that were similar to or shared amino acids with previously described opioid peptides. By the in silico analysis, 15 of the 27 assayed peptides were found to exert two or more activities, with Angiotensin-converting enzyme (ACE) inhibitory, antioxidant, and opioid being the most commonly found. The in vitro study confirmed ACE-inhibitory and antioxidant activities in 15 and 26 of the 27 synthetic peptides, respectively. Four fragments, RYLGYLE, YLGYLE, YFYPEL, and YPWT, also demonstrated the ability to protect Caco-2 and macrophages RAW264.7 cells from the oxidative damage caused by chemicals. The multifunctionality of these peptides makes them promising agents against oxidative stress-associated diseases.

Introduction to the Special Issue: New Advances in the Research of Antioxidant Food Peptides

“…Moreover, a more pronounced impact of conditions in the biochemical assays compared to the cellular antioxidant assay was observed. In the study of Amigo et al [6], an integrated approach combining in silico and in vitro assays was used, confirming the multifunctionality of milk and meat protein-derived peptides that were similar to or shared amino acids with previously described opioid peptides. Fifteen of twenty-seven assayed peptides were found to exert two or more activities, with angiotensin-converting enzyme (ACE) inhibitory, antioxidant, and opioid being the most commonly found.…”

Antioxidant Potential of the Sweet Whey-Based Beverage Colada after the Digestive Process and Relationships with the Lipid and Protein Fractions

“…The antioxidant activity of casein derived peptides has been extensively studied and described [ 63 ]. One of the peptides identified, YPFPGPI, which shares sequence with other peptides found, has been recently described as a potent antioxidant [ 65 ]. Although antioxidant peptides derived from milk proteins have been mostly associated with bovine casein, hydrolysis of whey proteins may also result in the production of antioxidant peptides [ 63 , 64 ].…”

The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico

“…Some studies have dealt with in vitro and in silico to obtain information on bioactive peptides. Even though in vitro and in vivo studies have been conducted to study antimicrobial activity, and small antimicrobial peptides could be explored [38], in silico and in vitro studies could also be used to confirm the multifunctionality of peptides [39].…”