In-silico design of a multivalent epitope-based vaccine against Candida auris

Akhtar, Nahid; Joshi, Amit; Kaushik, Vikas; Kumar, Manish; Mannan, M. Amin-ul

doi:10.1016/j.micpath.2021.104879

Cited by 47 publications

(38 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, there has been no report of development of vaccine candidate against canine circovirus by targeting its capsid and replicase protein by immunoinformatic approach so far. The in silico immunoinformatic approach has become the first step in developing vaccine candidates because of its cost-effective and time-saving manner [ 16 , 46 , 47 ]. This approach can also help to overcome limitations such as genetic variations, antigenic shifts, and antigenic drifts during the vaccine development process [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…The interaction of these epitopes with the DLA alleles was evaluated by molecular docking study. The negative binding energy between the interactions of epitopes with the DLA molecules implies stable interactions and good binding affinity [ 16 ]. Furthermore, the molecular dynamics simulation analysis also corroborated that the interactions among the epitopes and the DLA alleles were stable.…”

Section: Discussionmentioning

confidence: 99%

“…After the selection of the epitopes, they were linked by GGS linkers to adjuvants such as RS09 (APPHALS) and N and C terminal sequence of Salmonella dublin flagellin protein along with PADRE sequence to design the final vaccine construct. These linker and adjuvants have been previously used in multivalent epitopebased vaccine design of herpes simplex virus and human papillomavirus, Candida auris, human cytomegalovirus, and dengue virus [16,17,22,23,26,50]. PADRE (AKF-VAAWTLKAAA) sequence was added to enhance the stability and potency of the vaccine construct [50,51].…”

Section: Discussionmentioning

confidence: 99%

“…As, these proteins are highly conserved, are involved in viral pathogenesis, and can help to generate immune response, they can be a good target for identifying epitopes that could be used to design a vaccine construct which can help protect the dog population from potentially lethal canine circovirus infections [13][14][15]. Such in silico studies have been previously performed to design vaccine construct against dengue, Candida auris, human cytomegalovirus, SARS-CoV-2, Lassa virus, human papillomavirus, cervical cancer, and hepatitis C virus [16][17][18][19][20][21][22][23][24][25][26]. In our study, various immunoinformatic tools were used to design a potential vaccine construct against canine circovirus.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus

Jain

Joshi

Akhtar

et al. 2021

Journal of Genetic Engineering and Biotechnology

Self Cite

View full text Add to dashboard Cite

Background Canine circovirus is a deadly pathogen of dogs and causes vasculitis and hemorrhagic enteritis. It causes lethal gastroenteritis in pigs, fox, and dogs. Canine circovirus genome contains two main (and opposite) transcription units which encode two open reading frames (ORFs), a replicase-associated protein (Rep) and the capsid (Cap) protein. The replicase protein and capsid protein consist of 303 amino acids and 270 amino acids respectively. Several immuno-informatics methods such as epitope screening, molecular docking, and molecular-dynamics simulations were used to craft peptide-based vaccine construct against canine circovirus. Results The vaccine construct was designed by joining the selected epitopes with adjuvants by suitable linker. The cloning and expression of the vaccine construct was also performed using in silico methods. Screening of epitopes was conducted by NetMHC server that uses ANN (Artificial neural networking) algorithm. These methods are fast and cost-effective for screening epitopes that can interact with dog leukocyte antigens (DLA) and initiate an immune response. Overall, 5 epitopes, YQHLPPFRF, YIRAKWINW, ALYRRLTLI, HLQGFVNLK, and GTMNFVARR, were selected and used to design a vaccine construct. The molecular docking and molecular dynamics simulation studies show that these epitopes can bind with DLA molecules with stability. The codon adaptation and in silico cloning studies show that the vaccine can be expressed by Escherichia coli K12 strain. Conclusion The results suggest that the vaccine construct can be useful in preventing the dogs from canine circovirus infections. However, the results need further validation by performing other in vitro and in vivo experiments.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus

Jain

Joshi

Akhtar

et al. 2021

Journal of Genetic Engineering and Biotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, epitope mapping via immunoinformatics tools will exclude the non-antigenic regions of protein candidates, where only epitopes that stimulate B and T cells would be selected for the vaccine design, which would give a more potent immune response [ 77 ]. The approach of designing and validating a vaccine through a computational approach has been applied against many fungi such as Aspergillus fumigatus [ 78 ], Candida auris [ 79 ], and Candida albicans [ 18 ]. This approach had been validated with wet-lab experiments, and immunized mice were protected from fatal candidiasis [ 80 ].…”

Section: Discussionmentioning

confidence: 99%

In Silico Designing of a Multitope Vaccine against Rhizopus microsporus with Potential Activity against Other Mucormycosis Causing Fungi

Soltan

Eldeen

Elbassiouny

et al. 2021

Cells

View full text Add to dashboard Cite

During the current era of the COVID-19 pandemic, the dissemination of Mucorales has been reported globally, with elevated rates of infection in India, and because of the high rate of mortality and morbidity, designing an effective vaccine against mucormycosis is a major health priority, especially for immunocompromised patients. In the current study, we studied shared Mucorales proteins, which have been reported as virulence factors, and after analysis of several virulent proteins for their antigenicity and subcellular localization, we selected spore coat (CotH) and serine protease (SP) proteins as the targets of epitope mapping. The current study proposes a vaccine constructed based on top-ranking cytotoxic T lymphocyte (CTL), helper T lymphocyte (HTL), and B cell lymphocyte (BCL) epitopes from filtered proteins. In addition to the selected epitopes, β-defensins adjuvant and PADRE peptide were included in the constructed vaccine to improve the stimulated immune response. Computational tools were used to estimate the physicochemical and immunological features of the proposed vaccine and validate its binding with TLR-2, where the output data of these assessments potentiate the probability of the constructed vaccine to stimulate a specific immune response against mucormycosis. Here, we demonstrate the approach of potential vaccine construction and assessment through computational tools, and to the best of our knowledge, this is the first study of a proposed vaccine against mucormycosis based on the immunoinformatics approach.

show abstract

Candida auris, a singular emergent pathogenic yeast: its resistance and new therapeutic alternatives

Fernandes

Ribeiro

Henriques

et al. 2022

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

In-silico design of a multivalent epitope-based vaccine against Candida auris

Cited by 47 publications

References 60 publications

An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus

An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus

In Silico Designing of a Multitope Vaccine against Rhizopus microsporus with Potential Activity against Other Mucormycosis Causing Fungi

Candida auris, a singular emergent pathogenic yeast: its resistance and new therapeutic alternatives

Contact Info

Product

Resources

About