In silico study of bone tissue regeneration in an idealised porous hydrogel scaffold using a mechano-regulation algorithm

Zhao, Feihu; Garrigle, Myles J. Mc; Vaughan, Ted J.; McNamara, Laoise M.

doi:10.1007/s10237-017-0941-3

Cited by 20 publications

(14 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, recent studies are also indicating that interconnected pores are necessary to allow for the circulation of nutrients, waste and to promote the migration of cells to the center of the implant [ 225 , 226 ]. Thus, biomaterials scaffolds for BTE should exhibit both suitable pore sizes and porosity, as well as good interconnectivity among the pores [ 74 , 227 , 228 , 229 ].…”

Section: The Effect Of Substrate Topographymentioning

confidence: 99%

Topography: A Biophysical Approach to Direct the Fate of Mesenchymal Stem Cells in Tissue Engineering Applications

Cun

Hosta‐Rigau

2020

Nanomaterials

View full text Add to dashboard Cite

Tissue engineering is a promising strategy to treat tissue and organ loss or damage caused by injury or disease. During the past two decades, mesenchymal stem cells (MSCs) have attracted a tremendous amount of interest in tissue engineering due to their multipotency and self-renewal ability. MSCs are also the most multipotent stem cells in the human adult body. However, the application of MSCs in tissue engineering is relatively limited because it is difficult to guide their differentiation toward a specific cell lineage by using traditional biochemical factors. Besides biochemical factors, the differentiation of MSCs also influenced by biophysical cues. To this end, much effort has been devoted to directing the cell lineage decisions of MSCs through adjusting the biophysical properties of biomaterials. The surface topography of the biomaterial-based scaffold can modulate the proliferation and differentiation of MSCs. Presently, the development of micro- and nano-fabrication techniques has made it possible to control the surface topography of the scaffold precisely. In this review, we highlight and discuss how the main topographical features (i.e., roughness, patterns, and porosity) are an efficient approach to control the fate of MSCs and the application of topography in tissue engineering.

show abstract

Section: The Effect Of Substrate Topographymentioning

confidence: 99%

Topography: A Biophysical Approach to Direct the Fate of Mesenchymal Stem Cells in Tissue Engineering Applications

Cun

Hosta‐Rigau

2020

Nanomaterials

View full text Add to dashboard Cite

show abstract

“…This is made possible by coupling μCT imaging and numerical simulation. CFD approaches have shown promising results to estimate WSS distribution in idealized geometries and numerically reconstructed scaffold geometry from X‐ray tomography . To date, this strategy has been implemented with considerable success on single scaffold with a single‐cell resolution without contrast agent .…”

Section: Discussionmentioning

confidence: 99%

“…CFD approaches have shown promising results to estimate WSS distribution in idealized geometries and numerically reconstructed scaffold geometry from X-ray tomography. [33][34][35]55,56 To date, this strategy has been implemented with considerable success on single scaffold with a singlecell resolution without contrast agent. 28,29 This approach enables the assessment of cell proliferation (and mineralization) within the volume of 3D scaffolds.…”

Section: Discussionmentioning

confidence: 99%

“…Authors report a proof‐of‐principle, demonstrating that it is possible to model the real geometrical and hydro‐dynamical microenvironment within a scaffold and the presence of cells in micro‐pores. Various modelling approaches carried out within the last decade are reported in the literature . To evaluate the localized fluid shear stress distributions throughout the culture period from micro‐tomography images, giving an indication of the effects of tissue growth on the flow‐induced stress field, the authors deployed fluid dynamic simulations using a custom in‐house lattice Boltzmann (LBM) program .…”

Section: Introductionmentioning

confidence: 99%

“…Various modelling approaches carried out within the last decade are reported in the literature. [32][33][34][35] To evaluate the localized fluid shear stress distributions throughout the culture period from micro-tomography images, giving an indication of the effects of tissue growth on the flow-induced stress field, the authors deployed fluid dynamic simulations using a custom in-house lattice Boltzmann (LBM) program. 30 LBM easily handles complex geometries such as porous media.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Assessment of the interplay between scaffold geometry, induced shear stresses, and cell proliferation within a packed bed perfusion bioreactor

Thibeaux

Duval

Smaniotto

et al. 2019

Biotechnology Progress

View full text Add to dashboard Cite

By favoring cell proliferation and differentiation, perfusion bioreactors proved efficient at optimizing cell culture. The aim of this study was to quantify cell proliferation within a perfusion bioreactor and correlate it to the wall shear stress (WSS) distribution by combining 3‐D imaging and computational fluid dynamics simulations.NIH‐3T3 fibroblasts were cultured onto a scaffold model made of impermeable polyacetal spheres or Polydimethylsiloxane cubes. After 1, 2, and 3 weeks of culture, constructs were analyzed by micro‐computed tomography (μCT) and quantification of cell proliferation was assessed. After 3 weeks, the volume of cells was found four times higher in the stacking of spheres than in the stacking of cube.3D‐μCT reconstruction of bioreactors was used as input for the numerical simulations. Using a lattice‐Boltzmann method, we simulated the fluid flow within the bioreactors. We retrieved the WSS distribution (PDF) on the scaffolds surface at the beginning of cultivation and correlated this distribution to the local presence of cells after 3 weeks of cultivation. We found that the WSS distributions strongly differ between spheres and cubes even if the porosity and the specific wetted area of the stackings were very similar. The PDF is narrower and the mean WSS is lower for cubes (11 mPa) than for spheres (20 mPa). For the stacking of spheres, the relative occupancy of the surface sites by cells is maximal when WSS is greater than 20 mPa. For cubes, the relative occupancy is maximal when the WSS is lower than 10 mPa. The discrepancies between spheres and cubes are attributed to the more numerous sites in stacking of spheres that may induce 3‐D (multi‐layered) proliferation.

show abstract

Multiphysics simulation of a compression–perfusion combined bioreactor to predict the mechanical microenvironment during bone metastatic breast cancer loading experiments

Liu

Han

Modarres‐Sadeghi

et al. 2021

Biotech & Bioengineering

View full text Add to dashboard Cite

Incurable breast cancer bone metastasis causes widespread bone loss, resulting in fragility, pain, increased fracture risk, and ultimately increased patient mortality. Increased mechanical signals in the skeleton are anabolic and protect against bone loss, and they may also do so during osteolytic bone metastasis. Skeletal mechanical signals include interdependent tissue deformations and interstitial fluid flow, but how metastatic tumor cells respond to each of these individual signals remains underinvestigated, a barrier to translation to the clinic. To delineate their respective roles, we report computed estimates of the internal mechanical field of a bone mimetic scaffold undergoing combinations of high and low compression and perfusion using multiphysics simulations. Simulations were conducted in advance of multimodal loading bioreactor experiments with bone metastatic breast cancer cells to ensure that mechanical stimuli occurring internally were physiological and anabolic. Our results show that mechanical stimuli throughout the scaffold were within the anabolic range of bone cells in all loading configurations, were homogenously distributed throughout, and that combined high magnitude compression and perfusion synergized to produce the largest wall shear stresses within the scaffold. These simulations, when combined with experiments, will shed light on how increased mechanical loading in the skeleton may confer anti‐tumorigenic effects during metastasis.

show abstract

In silico study of bone tissue regeneration in an idealised porous hydrogel scaffold using a mechano-regulation algorithm

Cited by 20 publications

References 42 publications

Topography: A Biophysical Approach to Direct the Fate of Mesenchymal Stem Cells in Tissue Engineering Applications

Topography: A Biophysical Approach to Direct the Fate of Mesenchymal Stem Cells in Tissue Engineering Applications

Assessment of the interplay between scaffold geometry, induced shear stresses, and cell proliferation within a packed bed perfusion bioreactor

Multiphysics simulation of a compression–perfusion combined bioreactor to predict the mechanical microenvironment during bone metastatic breast cancer loading experiments

Contact Info

Product

Resources

About