In silico Study to Reveal Annotation and Significant Interactions of Human Defensin with its Isoforms and their Phylogeny

Arif, Rawaba; Ahmed, Sibtain; Mustafa, Ghulam

doi:10.36468/pharmaceutical-sciences.669

Cited by 5 publications

(3 citation statements)

References 34 publications

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“…Natural products obtained from different organisms are a very useful resource for improving health care issues and treating different ailments [25][26][27][28] . Fibrinolytic enzymes have been used in clinical treatments including prevention and cure of thrombotic diseases, regulation of cellular fibrinolysis where they act as Fig.…”

Section: Resultsmentioning

confidence: 99%

Isolation, Identification and Sequence Analysis of Subtilisin Gene (Quaking Homolog) Encoding a Fibrinolytic Enzyme from Bacillus subtilis

Mushtaq¹,

Showky²,

Irshad³

et al. 2021

ijps

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Mushtaq et al.: Characterization of Subtilisin Gene from Bacillus subtilisFibrinolytic therapy progressed by the evolution of different strategies that helped in enhancing its efficacy and specificity. The use of microbial fibrinolytic enzymes is leading to a promising direction for the treatment of cardiovascular diseases. Subtilisin, a member of subtilases is a fibrinolytic enzyme abundantly found in Bacillus species. The isolation of subtilisin gene (quaking homolog) from Bacillus subtilis was attempted in the present work. The genomic deoxyribonucleic acid extraction was done following Yamada protocol and used as a template for polymerase chain reaction amplification of the target gene using specifically designed primers. The polymerase chain reaction product was ligated into cloning vector pTZ57R/T followed by its transformation into Escherichia coli top 10 strain. A 1090 base pair, partial gene sequence was amplified coding for subtilisin protein of 363 amino acids with molecular weight of 37550.7 Daltons. The nucleotide sequence revealed significant evolutionary relationships with subtilases from other strains of Bacillus subtilis. Our study confirms the presence of subtilisin (quaking homolog) gene in local Bacillus species suggesting economical way to produce important thrombolytic agents of commercial and pharmaceutical worth.

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Section: Resultsmentioning

confidence: 99%

Isolation, Identification and Sequence Analysis of Subtilisin Gene (Quaking Homolog) Encoding a Fibrinolytic Enzyme from Bacillus subtilis

Mushtaq¹,

Showky²,

Irshad³

et al. 2021

ijps

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“…Phylogeny is an effective tool for determining the evolutionary relationships among various species in terms of protein and its functionality [23,24]. To further elucidate the evolutionary relationships of TLRs, a phylogram among all human TLR proteins was also reconstructed.…”

Section: Biomed Research Internationalmentioning

confidence: 99%

Sequence and Structural Characterization of Toll-Like Receptor 6 from Human and Related Species

Mustafa

Mahrosh

Arif

2021

BioMed Research International

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Toll-like receptors (TLRs) play an important role in the innate immune response against various pathogens. They serve as expected targets of natural selection in those species which are adapted to habitats with contrasting pathogen burdens. Till date, sufficient literature about TLRs especially TLR6 is not available. The current study was therefore planned to show evolutionary patterns of human TLRs generally and TLR6 specifically along with their conservation and diversity. The study also deals with characteristic polymorphic patterns of TLR6 in humans which are involved in serious clinical consequences. The sequence analysis of TLR6 from different mammals revealed conserved regions in the protein sequence. With respect to TLR6 evolution, human showed a close evolutionary relationship with chimpanzee and orangutans, while monkeys were appeared in a separate clade showing a distant evolutionary relationship. Old World monkeys and New World monkeys made their separate clades but both have evolved from a common ancestor. The C-terminal of human TLRs (TLR1 to TLR10) exhibited more conservation as compared to other regions. The phylogram of human TLRs showed that TLR6 is closely related to TLR1 and both TLRs shared a common ancestor with TLR10. The domain analysis has revealed that TLR1 and TLR10 have least (i.e., 4) number of leucine-rich repeat (LRR) while TLR6 contains five LRRs. Three single nucleotide polymorphisms were found in TLR6 which were found to be associated with benign. Conclusively, the current comparative sequence analyses and phylogenetic analyses provided informative insights into the process of TLR evolution in mammals. Furthermore, the polymorphism analysis would serve as a useful marker in the early detection of susceptibility and resistance against cancers and other diseases in humans.

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“…Today, plant research is the center of attraction because it has the potential to be used in various areas of the world's industry. A wide range of natural materials is used to maintain the health of all living beings [1] . About 80 % of the world's population still depends on traditional medicine.…”

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confidence: 99%

Evaluation of Crude and Modified Cordia myxa Gum for its Nutraceutical Benefits

Tahir¹,

Mustafa²,

Anjum³

et al. 2021

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Tahir et al.: Nutraceutical Benefits of Cordia myxa GumPlants are a rich source of safe and valuable bioactive compounds and since ancient times they have been used as medicines. Medicinal herbs are used as traditional means of treating many human diseases in many parts of the world. In current study, the green synthesis pathway was investigated for modification, reducing and crown potential of Cordia myxa gum for the synthesis of silver nanoparticles. The seeding process and the synthesis of silver nanoparticles were tracked by taking ultraviolet/visible absorption spectra during the reaction. Nutritional as well as medicinal potential of raw and modified gums were analyzed. Synthesized spherical nanoparticles were up to 65 nm in size. The ultraviolet/visible spectroscopic results revealed an intense peak at 428 nm together with other small peaks in the region of 390-450 nm due to the presence of nanoparticles of polydispersed silver. The purified gum showed the highest phenolic contents (i.e. 14.71±0.04 mg) and percentage scavenging activity (i.e. 87.92±2.25). The maximum bactericidal activity was showed by nanoformulated gum against Escherichia coli with inhibition zone of 42 mm while minimum was found to be against Staphylococcus aureus with inhibition zone of 14 mm. Nano formulated gum samples also exhibited optimal fungicidal effects against Fusarium solani with inhibition zone of 11.09 mm and Aspergillus niger with inhibition zone of 15.53 mm. The results of current study portrayed that modifications in Cordia myxa gum would be beneficial to food products as an additive.

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In silico Study to Reveal Annotation and Significant Interactions of Human Defensin with its Isoforms and their Phylogeny

Cited by 5 publications

References 34 publications

Isolation, Identification and Sequence Analysis of Subtilisin Gene (Quaking Homolog) Encoding a Fibrinolytic Enzyme from Bacillus subtilis

Isolation, Identification and Sequence Analysis of Subtilisin Gene (Quaking Homolog) Encoding a Fibrinolytic Enzyme from Bacillus subtilis

Sequence and Structural Characterization of Toll-Like Receptor 6 from Human and Related Species

Evaluation of Crude and Modified Cordia myxa Gum for its Nutraceutical Benefits

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