2018
DOI: 10.1007/s00709-018-1316-z
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In situ assessment of mitochondrial calcium transport in tobacco pollen tubes

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Cited by 7 publications
(7 citation statements)
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“…KEGG enrichment analysis showed that the up-regulated proteins were significantly enriched in Oxidative phosphorylation signaling pathways. Oxidative phosphorylation plays an important role in maintaining pollen tube development and pollen fertility, and is the basis of regulating pollen formation and development (Flores-Herrera et al, 2019;Wei et al, 2020). In addition, MBF1 and cysteine proteinase inhibitor were most significantly up-regulated during pollen maturation.…”
Section: Discussionmentioning
confidence: 99%
“…KEGG enrichment analysis showed that the up-regulated proteins were significantly enriched in Oxidative phosphorylation signaling pathways. Oxidative phosphorylation plays an important role in maintaining pollen tube development and pollen fertility, and is the basis of regulating pollen formation and development (Flores-Herrera et al, 2019;Wei et al, 2020). In addition, MBF1 and cysteine proteinase inhibitor were most significantly up-regulated during pollen maturation.…”
Section: Discussionmentioning
confidence: 99%
“…Protoplast permeability was determined under the microscope after staining for 5 minutes with 1% Evans blue followed by extensive washing with PAB. 35 …”
Section: Methodsmentioning
confidence: 99%
“…Recent reports have successfully measured Mitochondrial Calcium Uniporter-dependent calcium uptake activity either using electrophysiology of reconstituted MCU or through heterologous expression of MCU in yeast [2,3]. Here we present methodological details to assess in situ mitochondrial respiration and calcium uptake in permeabilized pollen tubes [4]. This method could be applied -in principle- to pollen tubes from any plant provided adequate germination and assay buffers are used throughout the process.…”
Section: Methods Detailsmentioning
confidence: 99%