In situ attenuated total reflectance FT-IR analysis of an enzyme-modified mid-infrared fiber surface using crystalline bacterial surface proteins

Taga, K.; Kellner, Robert; Kainz, Ursula; Sleytr, Uwe B.

doi:10.1021/ac00073a008

Cited by 32 publications

(22 citation statements)

References 18 publications

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“…[270] Fiber sensor for general anesthetics, Raman spectroscopy [310] Mid-IR accessible for molecular spectr. and gas anal., tunable diode lasers [414] In situ ATR FT-IR analysis of enzyme-modified fiber [418] pH meter using NIR dye (NIR proton-carrier dye and laser diodes)…”

Section: Optical Principlesmentioning

confidence: 99%

Opto-Chemical and Opto-Immuno Sensors

Gauglitz¹

1996

Sensors Update

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Section: Optical Principlesmentioning

confidence: 99%

Opto-Chemical and Opto-Immuno Sensors

Gauglitz¹

1996

Sensors Update

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“…The tapered fiber is functionalized for antibody detection by immobilization of an antigen sensor layer in the outer surface of the taper waist of the fiber. This antigen layer can be the crystalline bacterial surface layer, known as S layer, which is a common technique for functionalization of chalcogenide surfaces for immobilizing functional biomolecules [41]. The thickness of the sensing S layer has been experimentally determined to be ∼40 nm with refractive index of 1.45 [41], [42].…”

Section: Sensor Configurationmentioning

confidence: 99%

“…This antigen layer can be the crystalline bacterial surface layer, known as S layer, which is a common technique for functionalization of chalcogenide surfaces for immobilizing functional biomolecules [41]. The thickness of the sensing S layer has been experimentally determined to be ∼40 nm with refractive index of 1.45 [41], [42]. For our investigation, we consider big biomolecules to be able to have a detectable effect as shown in Fig.…”

Section: Sensor Configurationmentioning

confidence: 99%

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Nonlinear Label-Free Biosensing With High Sensitivity Using As<sub>2</sub>S<sub>3</sub> Chalcogenide Tapered Fiber

Markos

Bang

2015

J. Lightwave Technol.

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We demonstrate an experimentally feasible fiber design, which can act as a highly sensitive, label-free, and selective biosensor using the inherent high nonlinearity of an As 2 S 3 chalcogenide tapered fiber. The surface immobilization of the fiber with an antigen layer can provide the possibility to selectively capture antibody biomolecules. This increase of the layer thickness directly affects the group velocity dispersion of the fiber and, thus, the modulation instability (MI) gain spectrum changes (location of the anti-Stokes and Stokes wavelengths) when pumping the fiber close to the zero-dispersion wavelength. The sensitivity of the sensor was predicted to be ∼18 nm/nm, defined as the shift in resonance wavelength per nanometer biolayer thickness, which is almost twice the current record sensitivity of nonlinear MI-based fiber optical biosensors. Importantly, due to the strong nonlinearity of As 2 S 3 , this high sensitivity can be obtained using a low-power 1064-nm microchip laser.

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“…But besides thermal imaging, the mid-infrared domain is of great interest for vibrational spectroscopy of chemicals and biomolecules [4][5][6][7][8][9]. Indeed, almost all molecules exhibit highly specific vibrational signatures in the 2-16 microns range which provides an effective mean of performing selective optical sensing.…”

Section: Introductionmentioning

confidence: 99%

Chalcogenide glass fibers: Optical window tailoring and suitability for bio-chemical sensing

et al. 2015

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a b s t r a c tGlassy materials based on chalcogen elements are becoming increasingly prominent in the development of advanced infrared sensors. In particular, infrared fibers constitute a desirable sensing platform due to their high sensitivity and versatile remote collection capabilities for in-situ detection. Tailoring the transparency window of an optical material to the vibrational signature of a target molecule is important for the design of infrared sensor, and particularly for fiber evanescent wave spectroscopy. Here we review the basic principles and recent developments in the fabrication of chalcogenide glass infrared fibers for application as bio-chemical sensors. We emphasize the challenges in designing materials that combine good rheological properties with chemical stability and sufficiently wide optical windows for bio-chemical sensing. The limitation in optical transparency due to higher order overtones of the amorphous network vibrations is established for this family of glasses. It is shown that glasses with wide optical window suffer from higher order overtone absorptions. Compositional engineering with heavy elements such as iodine is shown to widen the optical window but at the cost of lower chemical stability. The optical attenuations of various families of chalcogenide glass fibers are presented and weighed for their applications as chemical sensors. It is then shown that long-wave infrared fibers can be designed to optimize the collection of selective signal from bio-molecules such as cells and tissues. Issues of toxicity and mechanical resistance in the context of bio-sensing are also discussed.

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In situ attenuated total reflectance FT-IR analysis of an enzyme-modified mid-infrared fiber surface using crystalline bacterial surface proteins

Cited by 32 publications

References 18 publications

Opto-Chemical and Opto-Immuno Sensors

Opto-Chemical and Opto-Immuno Sensors

Nonlinear Label-Free Biosensing With High Sensitivity Using As<sub>2</sub>S<sub>3</sub> Chalcogenide Tapered Fiber

Chalcogenide glass fibers: Optical window tailoring and suitability for bio-chemical sensing

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