In situ biostimulation of petroleum hydrocarbon degradation by nitrate and phosphate injection using a dipole well configuration

Ponsin, Violaine; Coulomb, Bruno; Guelorget, Yves; Maier, Joachim; Höhener, Patrick

doi:10.1016/j.jconhyd.2014.10.003

Cited by 24 publications

(18 citation statements)

References 28 publications

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“…Bioremediation is often enhanced by supplying additional nutrients or oxygen to promote microbial activity [30]. In particular, at hydrocarbon-contaminated sites, adjustment of the C/N/P ratio is important for efficient bioremediation because of the excess amount of carbon [31].…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms of Enhanced Bacterial Growth on Hexadecane with Red Clay

et al. 2015

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Red clay was previously used to enhance bioremediation of diesel-contaminated soil. It was speculated that the enhanced degradation of diesel was due to increased bacterial growth. In this study, we selected Acinetobacter oleivorans DR1, a soil-borne degrader of diesel and alkanes, as a model bacterium and performed transcriptional analysis using RNA sequencing to investigate the cellular response during hexadecane utilization and the mechanism by which red clay promotes hexadecane degradation. We confirmed that red clay promotes the growth of A. oleivorans DR1 on hexadecane, a major component of diesel, as a sole carbon source. Addition of red clay to hexadecane-utilizing DR1 cells highly upregulated β-oxidation, while genes related to alkane oxidation were highly expressed with and without red clay. Red clay also upregulated genes related to oxidative stress defense, such as superoxide dismutase, catalase, and glutaredoxin genes, suggesting that red clay supports the response of DR1 cells to oxidative stress generated during hexadecane utilization. Increased membrane fluidity in the presence of red clay was confirmed by fatty acid methyl ester analysis at different growth phases, suggesting that enhanced growth on hexadecane could be due to increased uptake of hexadecane coupled with upregulation of downstream metabolism and oxidative stress defense. The monitoring of the bacterial community in soil with red clay for a year revealed that red clay stabilized the community structure.

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Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms of Enhanced Bacterial Growth on Hexadecane with Red Clay

et al. 2015

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“…The forced gradient induced by the doublet creates a local recirculation zone whose shape depends on the hydraulic properties of the aquifer and on the regional flow strength and orientation. The tracer tests performed in these conditions are of interest for management of sea water intrusion (Sheahan, 1977), groundwater remediation (Ponsin et al, 2014), reactivity characterization (Burbery et al, 2013) or characterization of hydraulic and transport parameters (Clement et al, 1997;Grove and Beetem, 1971).…”

Section: Steady-state Analytical Rtdsmentioning

confidence: 99%

Residence time distributions for hydrologic systems: Mechanistic foundations and steady-state analytical solutions

Leray

Engdahl

Massoudieh

et al. 2016

Journal of Hydrology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Steady-state analytical RTDs contaminant transport, and ecosystem preservation. Analytical solutions are often adopted as a model of the RTD and a broad spectrum of models from many disciplines has been applied. Although these solutions are typically reduced in dimensionality and limited in complexity, their ease of use makes them preferred tools, specifically for the interpretation of tracer data. Our review begins with the mechanistic basis for the governing equations, highlighting the physics for generating a RTD, and a catalog of analytical solutions follows. This catalog explains the geometry, boundary conditions and physical aspects of the hydrologic systems, as well as the sampling conditions, that altogether give rise to specific RTDs. The similarities between models are noted, as are the appropriate conditions for their applicability. The presentation of simple solutions is followed by a presentation of more complicated analytical models for RTDs, including serial and parallel combinations, lagged systems, and non-Fickian models. The conditions for the appropriate use of analytical solutions are discussed, and we close with some thoughts on potential applications, alternative approaches, and future directions for modeling hydrologic residence time.

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“…However, the general trend of dissolved benzene concentrations showed also a marked decrease during the four-year period studied, suggesting that physical and tentatively biological natural attenuation was very effective on the site. Complementary field and lab data confirm that benzene concentrations were rapidly lowered to below detection limit in a field test and in microcosms where nitrate and phosphate were added (Ponsin et al, 2014a(Ponsin et al, , 2014b. Further strong evidence for toluene and benzene biodegradation is given by the results of carbon isotope ratios in these compounds.…”

Section: Hydrocarbon Degradationmentioning

confidence: 60%

“…6). A dipole test with injection and recirculation of nutrients (nitrate and phosphate) was conducted in Pz21 and Pz25 between months 21 and 25 after the spill (Ponsin et al, 2014a). Dissolved nitrate and phosphate decreased rapidly to non-detectable concentration after month 26 in this zone.…”

Section: Temporal Geochemical Evolution Of the Source Zonementioning

confidence: 99%

“…Within a time frame starting seven months after the spill and ending 53 months after the spill, a total of 2047 kg of hydrocarbons (CH 1.85 ) were degraded on site, to which 115 kg from a recirculating tracer test should be added (Ponsin et al, 2014a), for a total of 2162 kg (2.51 m 3 ). Within the same time frame, 900 kg of hydrocarbons (1.05 m 3 ) were trapped in the activated charcoal filter, and 30,616 kg (35.6 m 3 ) of LNAPL were recovered by skimming (unpublished classified report).…”

Section: Carbon Mass Balancementioning

confidence: 99%

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Documentation of time-scales for onset of natural attenuation in an aquifer treated by a crude-oil recovery system

Ponsin

Maier²,

Guelorget³

et al. 2015

Science of The Total Environment

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International audienceA pipeline transporting crude-oil broke in a nature reserve in 2009 and spilled 5100 m(3) of oil that partly reached the aquifer and formed progressively a floating oil lens. Groundwater monitoring started immediately after the spill and crude-oil recovery by dual pump-and-skim technology was operated after oil lens formation. This study aimed at documenting the implementation of redox-specific natural attenuation processes in the saturated zone and at assessing whether dissolved compounds were degraded. Seven targeted water sampling campaigns were done during four years in addition to a routine monitoring of hydrocarbon concentrations. Liquid oil reached the aquifer within 2.5 months, and anaerobic processes, from denitrification to reduction of sulfate, were observable after 8 months. Methanogenesis appeared on site after 28 months. Stable carbon isotope analyses after 16 months showed maximum shifts in delta C-13 of +4.9 +/- 0.22 parts per thousand for toluene, +2.4 +/- 0.19 parts per thousand for benzene and +0.9 +/- 0.51 parts per thousand for ethylbenzene, suggesting anaerobic degradation of these compounds in the source zone. Estimations of fluxes of inorganic carbon produced by biodegradation revealed that, in average, 60% of inorganic carbon production was attributable to sulfate reduction. This percentage tended to decrease with time while the production of carbon attributable to methanogenesis was increasing. Within the investigation time frame, mass balance estimations showed that biodegradation is a more efficient process for control of dissolved concentrations compared to pumping and filtration on an activated charcoal filter. (C) 2015 Elsevier B.V. All rights reserved

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In situ biostimulation of petroleum hydrocarbon degradation by nitrate and phosphate injection using a dipole well configuration

Cited by 24 publications

References 28 publications

Molecular Mechanisms of Enhanced Bacterial Growth on Hexadecane with Red Clay

Molecular Mechanisms of Enhanced Bacterial Growth on Hexadecane with Red Clay

Residence time distributions for hydrologic systems: Mechanistic foundations and steady-state analytical solutions

Documentation of time-scales for onset of natural attenuation in an aquifer treated by a crude-oil recovery system

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