In situ detoxification of lignocellulosic hydrolysate using a surfactant for butyric acid production by Clostridium tyrobutyricum ATCC 25755

Lee, Kyung Min; Kim, Ki Yeon; Choi, Okkyoung; Woo, Han Min; Kim, Yunje; Han, Sung Ok; Sang, Byoung In; Um, Youngsoon

doi:10.1016/j.procbio.2015.01.020

Cited by 26 publications

(7 citation statements)

References 23 publications

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“…Results of Lee et al (2015) corroborated the previous assertion by verifying that phenolic compounds concentration of 0.87 g/L in the rice straw hydrolysate inhibited the production of butyric acid by C. tyrobutyricum ATCC 25755, indicating high toxicity of these products to the microorganism. In current work 0.88 g/L phenolics were observed at 15 % w/v biomass load.…”

Section: Liquid Hot Water Pretreatment (Lhw)supporting

confidence: 85%

Biobutanol production from sugarcane straw: Defining optimal biomass loading for improved ABE fermentation

Pratto

Chandgude

Sousa

et al. 2020

Industrial Crops and Products

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Section: Liquid Hot Water Pretreatment (Lhw)supporting

confidence: 85%

Biobutanol production from sugarcane straw: Defining optimal biomass loading for improved ABE fermentation

Pratto

Chandgude

Sousa

et al. 2020

Industrial Crops and Products

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“…Detoxification methods such as the use of lime, peroxidases, activated charcoal, surfactant and ion-exchange resin adsorption [15][16][17][18] have been proposed to reduce the hydrolysates toxicity. Despite its effectiveness, the detoxification process involves a series of separation and purification steps that can sharply increase the overall cost of the process and limit its economic feasibility [19].…”

Section: Introductionmentioning

confidence: 99%

Enhancing acetic acid and 5‐hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution

Alves

Zetty-Arenas

Demirci

et al. 2021

Process Biochemistry

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In this study, adaptive laboratory evolution (ALE) was applied to isolate four strains of Clostridium saccharoperbutylacetonicum able to grow in the presence of hemicellulosic hydrolysate inhibitors unsupported by the parental strain. Among them, isolate RAC-25 presented the best fermentative performance, producing 22.1 g/L of ABE and 16.7 g/L of butanol. Genome sequencing revealed a deletion in the arabinose transcriptional repressor gene (araR) and a mutation in the anti-sigma factor I that promoted a downregulation of sigI. Gene expression analysis indicated high expression of genes related to H +-pumps (ATP synthases), proline biosynthesis (gamma phosphate reductase) and chaperonins (Grol), suggesting an integrated mechanism that is probably coordinated by the repression of sigI. Therefore, in addition to highlighting the power of ALE for selecting robust strains, our results suggest that sigI and araR may be interesting gene targets for increased tolerance toward inhibitor compounds relevant for lignocellulosic biofuels production.

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“…Although washing only requires water, dilution of substrate concentration increases the total annualized costs (TAC) and reduces the energy efficiency of in situ recovery processes for ABE production [8]. Other detoxification methods before fermentation include extraction, adsorption, evaporation, electrodialysis, over-liming, neutralization, steam stripping, enzymatic and microbial treatment [22,23].…”

Section: Introductionmentioning

confidence: 99%

Economic optimization of in situ extraction of inhibitors in acetone-ethanol-butanol (ABE) fermentation from lignocellulose

Díaz

Tost

2018

Process Biochemistry

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The inhibitors produced in the pretreatment (phenolics, furans, and organic acids) as well as in the fermentation (butanol and organic acids) limit acetone, butanol, and ethanol (ABE) production from lignocellulose. To reduce their negative impact on the fermentation, a process involving simultaneous saccharification, ABE fermentation, and detoxification by liquid-liquid extraction was proposed and economically optimized. Although several extractants may be used to reduce butanol toxicity increasing the reactor performance, simultaneous detoxification of inhibitors from pretreatment (IFP) is difficult due to their high boiling point. Hence, the simultaneous detoxification system was evaluated using the biocompatible extractant: oleyl alcohol (boiling point of ~350 °C). Given that oleyl alcohol and IFP have a high boiling point, a heat-integrated distillation system with low-pressure columns was proposed to reduce the energy requirements of the purification of IFP and products from the fermentation. The simulations were performed rigorously in Aspen Plus ® and Matlab ® at different concentrations of IFP. Although IFP and butyric acid production become feasible ABE production by SSF-E at concentrations of IFP between 13.5 and 30 g/l, the energy requirements were between 1.2-and 2.4-fold higher than that for IFP concentrations of 9 g/l, respectively.

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In situ detoxification of lignocellulosic hydrolysate using a surfactant for butyric acid production by Clostridium tyrobutyricum ATCC 25755

Cited by 26 publications

References 23 publications

Biobutanol production from sugarcane straw: Defining optimal biomass loading for improved ABE fermentation

Biobutanol production from sugarcane straw: Defining optimal biomass loading for improved ABE fermentation

Enhancing acetic acid and 5‐hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution

Economic optimization of in situ extraction of inhibitors in acetone-ethanol-butanol (ABE) fermentation from lignocellulose

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