In situ Enabling Approaches for Tissue Regeneration: Current Challenges and New Developments

Dias, Juliana R.; Ribeiro, Nilza; Baptista-Silva, Sara; Costa-Pinto, Ana Rita; Alves, Nuno; Oliveira, Ana L.

doi:10.3389/fbioe.2020.00085

Cited by 42 publications

(24 citation statements)

References 158 publications

(255 reference statements)

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“…In situ tissue engineering is a powerful strategy for the treatment of bone defects; in this strategy, biomaterials are designed to mobilize endogenous stem cells to the site of injury and provide a highly osteoinductive environment [ 17 , 31 ]. In this study, we developed an ECM-based scaffold loaded with bioactive molecules to induce bone regeneration in situ , which sequentially delivered double factors in specific growth stages to enhance stem cells homing and colonization, as well as direct cell differentiation to osteogenic lineage.…”

Section: Discussionmentioning

confidence: 99%

In situ bone regeneration with sequential delivery of aptamer and BMP2 from an ECM-based scaffold fabricated by cryogenic free-form extrusion

Sun

Meng

Ding

et al. 2021

Bioactive Materials

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In situ tissue engineering is a powerful strategy for the treatment of bone defects. It could overcome the limitations of traditional bone tissue engineering, which typically involves extensive cell expansion steps, low cell survival rates upon transplantation, and a risk of immuno-rejection. Here, a porous scaffold polycaprolactone (PCL)/decellularized small intestine submucosa (SIS) was fabricated via cryogenic free-form extrusion, followed by surface modification with aptamer and PlGF-2 123-144 *-fused BMP2 (pBMP2). The two bioactive molecules were delivered sequentially. The aptamer Apt19s, which exhibited binding affinity to bone marrow-derived mesenchymal stem cells (BMSCs), was quickly released, facilitating the mobilization and recruitment of host BMSCs. BMP2 fused with a PlGF-2 123-144 peptide, which showed “super-affinity” to the ECM matrix, was released in a slow and sustained manner, inducing BMSC osteogenic differentiation. In vitro results showed that the sequential release of PCL/SIS-pBMP2-Apt19s promoted cell migration, proliferation, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes. The in vivo results demonstrated that the sequential release system of PCL/SIS-pBMP2-Apt19s evidently increased bone formation in rat calvarial critical-sized defects compared to the sequential release system of PCL/SIS-BMP2-Apt19s. Thus, the novel delivery system shows potential as an ideal alternative for achieving cell-free scaffold-based bone regeneration in situ .

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Section: Discussionmentioning

confidence: 99%

In situ bone regeneration with sequential delivery of aptamer and BMP2 from an ECM-based scaffold fabricated by cryogenic free-form extrusion

Sun

Meng

Ding

et al. 2021

Bioactive Materials

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“…In contrast, in vivo bioprinting has proven effective for injection of cell-laden bioinks at the defect site. [108] Its clinical translation is advantageous for scanning the defect area and deposition into the wound site over conventional 3D construct implantation, while eliminating the need to reshape the scaffold according to defect geometry. This aspect is highly desirable for irregular shapes and architectures.…”

Section: In Vivo Bioprintingmentioning

confidence: 99%

“…[120] However, existing drawbacks are clogging due to high cell density, nonuniform droplet sizes, and limited material choice due to viscosity requirements (<10 mPa s −1 ). [108] Enhanced cell viability can be achieved by laser induced forward transfer (LIFT), enabling deposition at the individual cell level (Figure 4C). Focused laser pulses trigger evaporation of the absorbing layer, propelling the bioink toward the site of interest.…”

Section: In Vivo Bioprintingmentioning

confidence: 99%

Controlling Structure with Injectable Biomaterials to Better Mimic Tissue Heterogeneity and Anisotropy

Babu

Albertino

Anarkoli

et al. 2021

Adv Healthcare Materials

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Tissue regeneration of sensitive tissues calls for injectable scaffolds, which are minimally invasive and offer minimal damage to the native tissues. However, most of these systems are inherently isotropic and do not mimic the complex hierarchically ordered nature of the native extracellular matrices. This review focuses on the different approaches developed in the past decade to bring in some form of anisotropy to the conventional injectable tissue regenerative matrices. These approaches include introduction of macroporosity, in vivo pattering to present biomolecules in a spatially and temporally controlled manner, availability of aligned domains by means of self-assembly or oriented injectable components, and in vivo bioprinting to obtain structures with features of high resolution that resembles native tissues. Toward the end of the review, different techniques to produce building blocks for the fabrication of heterogeneous injectable scaffolds are discussed. The advantages and shortcomings of each approach are discussed in detail with ideas to improve the functionality and versatility of the building blocks.

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“…Although this combination does not confer structural strength, it brings functionality and coating and must be combined with dilators in order to confer physical structure. Recently studies described the current belief is that biological materials used in vaginal construction are expected to provide a protective layer and allow tissue to undergo epithelization (Dias et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…Regardless of the fabrication approach used, scaffolds have a key role in the integration in new tissue having a crucial performance in the host microenvironment (Dias et al, 2020 ), although the barrier to scaffold translation demands specific and appropriated conditions to successfully reconstruct tissue and organs defects (Naderi et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Scaffold strategies combined with mesenchymal stem cells in vaginal construction: a review

et al. 2021

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Tissue engineering has provided new treatment alternatives for tissue reconstruction. Advances in the tissue engineering field have resulted in mechanical support and biological substitutes to restore, maintain or improve tissue/organs structures and functions. The application of tissue engineering technology in the vaginal reconstruction treatment can not only provide mechanical requirements, but also offer tissue repairing as an alternative to traditional approaches. In this review, we discuss recent advances in cell-based therapy in combination with scaffolds strategies that can potentially be adopted for gynaecological transplantation.

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In situ Enabling Approaches for Tissue Regeneration: Current Challenges and New Developments

Cited by 42 publications

References 158 publications

In situ bone regeneration with sequential delivery of aptamer and BMP2 from an ECM-based scaffold fabricated by cryogenic free-form extrusion

In situ bone regeneration with sequential delivery of aptamer and BMP2 from an ECM-based scaffold fabricated by cryogenic free-form extrusion

Controlling Structure with Injectable Biomaterials to Better Mimic Tissue Heterogeneity and Anisotropy

Scaffold strategies combined with mesenchymal stem cells in vaginal construction: a review

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