In-Situ-Generated Vasoactive Intestinal Peptide Loaded Microspheres in Mussel-Inspired Polycaprolactone Nanosheets Creating Spatiotemporal Releasing Microenvironment to Promote Wound Healing and Angiogenesis

Abstract

Vasoactive intestinal peptide (VIP) was reported to promote angiogenesis. Electrospun nanofibers lead to idea wound dressing substrates. Here we report a convenient and novel method to produce VIP loaded microspheres in polycaprolactone (PCL) nanofibrous membrane without complicated processes. We first coated mussel-inspired dopamine (DA) to nanofibers, then used strong adhesive DA to absorb the functional peptide. PCL membrane was then immersed into acetone to generate microspheres with VIP loading. We employed high pressure liquid chromatography to record encapsulation efficiency of (31.8 ± 2.2)% and loading capacity of (1.71 ± 0.16)%. The release profile of VIP from nanosheets showed a prolonged release. The results of laser scanning confocal microscope, scanning electron microscope and cell counting kit-8 proliferation assays showed that cell adhesion and proliferation were promoted. In order to verify the efficacy on wound healing, in vivo implantation was applied in the full-thickness defect wounds of BALB/c mice. Results showed that the wound healing was significantly promoted via favoring the growth of granulation tissue and angiogenesis. However, we found wound re-epithelialization was not significantly improved. The resulting VIP-DA-coated PCL (PCL-DA-VIP) nanosheets with spatiotemporal delivery of VIP could be a potential application in wound treatment and vascular tissue engineering.

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

“…33,34 BALB/c mice were individually anesthetized using an intraperitoneal injection of 1% pentobarbital; then, the dorsal surface was shaved. After rinsed with 75% alcohol, two 6 mm-diameter full-thickness wounds were prepared using a sterile 6 mm punch biopsy tool on either side of the back.…”

“…In PCL spectrum, two characteristic absorption peaks at 1,166 cm -1 and 1,726 cm -1 were related to C-O-C and C=O bonds, and peaks at 2,864 cm -1 and 2,930 cm -1 corresponded to the C-H bond. 33,38,39 After DA coating, a broad peak around 3,100-3,600 cm -1 corresponding to the stretching vibration band of phenolic hydroxyls was observed in the spectrum of PCL/DA. 13 The peak became sharper in the PCL/NS0.5, PCL/NS1.0 and PCL/NS2.0 spectra, suggesting the interaction between NS and DA.…”

Nano-silver-decorated microfibrous eggshell membrane: processing, cytotoxicity assessment and optimization, antibacterial activity and wound healing

“…To further investigate the underlying mechanism of the observed effects, PCNA was selected as a cell proliferation marker and was detected by both immunohistochemistry and Western blotting at day 3 post-surgery 38 . As shown in the immunohistochemical images (Fig.…”

New insights into the roles of myofibroblasts and innervation during skin healing and innovative therapies to improve scar innervation

“…A possible source of human neuropeptides, ideally from sensory neurones, could be generated using skin‐derived precursors (SKPs) or induced pluripotent stem cells (iPS) differentiated into neurones . Due to the distinct role of different neuropeptides and NTs, the use of a cocktail would be interesting with delivery either by single administration or ideally by delayed delivery into the wound . Thus, different delivery systems are possible via topical application or using vehicles such as microspheres, hydrogel or gelatin .…”