In Situ Growth of Porous Platinum Nanoparticles on Graphene Oxide for Colorimetric Detection of Cancer Cells

Abstract

A green approach is proposed for in situ growth of porous platinum nanoparticles on graphene oxide (PtNPs/GO). The resulting nanocomposite has been proven to function as peroxidase mimetics that can catalyze the reaction of peroxidase substrate in the presence of hydrogen peroxide. On the basis of the peroxidase-like activity, we used the PtNPs/GO as a signal transducer to develop a colorimetric assay for the direct detection of cancer cells. By using folic acid as a recognition element, a total of 125 cancer cells (MCF-7) can be distinguished by naked-eye observation. We envision that this nanomaterial could be used as a power tool for a wide range of potential applications in biotechnology and medicine.

Honeycomb-like nitrogen-doped porous carbon supporting Pt nanoparticles as enzyme mimic for colorimetric detection of cholesterol

“…A low K m represents a strong affinity and vice versa [36]. In our experiment, steadystate kinetic parameters of Pt/PCN were determined and compared with different materials, such as porous platinum nanoparticles on graphene oxide (Pt/GO) [37], ferritin-platinum (Pt/Ft) [38] and irregular-shaped platinum nanoparticles (ISPtNPs) [39] ( Table 1). The results show that the K m value of Pt/PCN with H 2 O 2 as the substrate is much lower than that of the other three materials, suggesting that Pt/PCN has a significant higher affinity to H 2 O 2 than the other three materials.…”

“…As the most known one, noble metal platinum (Pt) is chemically inert at bulk scales but can serve as the important catalysts at nanoscales because of their unique size, shape and the increased specific surface area [11]. Many reports demonstrated that Pt nanoparticles can exhibit peroxidase-like catalysis activity for the detection of H 2 O 2 [12,13]. Regrettably, Pt is a precious metal and pure Pt nanoparticles are usually instable and easily aggregated together, which limits the catalytic performance of Pt nanoparticles.…”

Self-templated formation of aptamer-functionalized copper oxide nanorods with intrinsic peroxidase catalytic activity for protein and tumor cell detection

“…Enzyme-like nanomaterials (nanozymes) [1,2] based on metallic [3][4][5], metal oxide [6][7][8][9][10], bimetallic nanoparticles (NPs) and nanoclusters (NCs) [11][12][13][14], carbon nanomaterials [15][16][17], and hybrid nanocomposites [18][19][20][21][22][23][24][25], have recently received a great deal of attention for their biochemical applications. Enzyme-mimicking molecules (e.g., metal complexes, hemin and other porphyrins, cyclodextrins, and supramolecules) also have been reported to have many advantages over natural enzymes [26][27][28].…”

Graphene based nanomaterials as chemical sensors for hydrogen peroxide – A comparison study of their intrinsic peroxidase catalytic behavior

“…Au@Pd bimetallic nanoparticles dispersed on graphene also demonstrated their ability as catalysts [14]. Gold nanoparticles on citrate functionalized graphene nanosheets [15], porous platinum nanoparticles growth in situ on graphene oxide [16] and magnetite nanospheres on reduced graphene oxide nanocomposites [17] were also synthesized and evaluated for their peroxidase mimic. Some graphene based nanomaterials without metallic nanoparticles were also investigated, among them being carboxyl modified graphene oxide [18] and few-layer graphene exfoliated by chitosan [19].…”