In situ hybridization of human immunodeficiency virus (HTLV‐III) in cryostat sections of lymph nodes of lymphadenopathy syndrome patients

Abstract

The presence of human immunodeficiency virus (HIV, or HTLV-III) genome sequence was investigated by means of in situ hybridization in cryostat sections of lymph nodes from lymphadenopathy syndrome (LAS) patients. The technique employed involved the modification of the DNA probe by chemical insertion of an antigenic sulfone group in cytosine moieties and the visualization of DNA by a double-antibody immunohistochemical reaction. The hybrid formation was revealed in five out of ten cases: in all positive samples, HIV was mainly observed in the cytoplasm of lymph node cells. The method of in situ hybridization described in the present paper is specific and has some advantages if compared with other techniques based on the use of DNA probes labelled with radioisotopes or biotin by nick translation.

Direct Visualization of HIV-1 Replication Intermediates Shows that Capsid and CPSF6 Modulate HIV-1 Intra-nuclear Invasion and Integration

“…Importantly, antibody-based imaging of HIV-1 and fluorescence in situ hybridization (FISH) strategies to visualize vRNA and vDNA (Pezzella et al, 1987; Singer et al, 1989), as well as the use of chimeric viral proteins (Campbell and Hope, 2008; Francis et al, 2014), have also improved our knowledge of HIV-1 infection at a single cell level. Some of the acknowledged limitations of these approaches are a lack of sensitivity and disruptive preparative conditions (i.e.…”

Diagnostic deoxyribonucleic acid probes for infectious diseases

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Treatment-Induced Decline of Human Immunodeficiency Virus-1 p24 and HIV-1 RNA in Lymphoid Tissue of Patients with Early Human Immunodeficiency Virus-1 Infection

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