In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio)

López, Mauricio; Figueroa, Jaime; Kausel, Gudrun; Vera, María; Krauskopf, Manuel

doi:10.4067/s0716-97602001000100010

Cited by 10 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Expression of SL is up/down-regulated during various internal/external environmental changes, including sexual maturation , acute stress (Rand-Weaver et al, 1993; but see Zhu and Thomas, 1995), salt/gas concentration (Kakizawa et al, 1993; but see Tang et al, 2001), season (RandWeaver et al, 1995; but see López et al, 2001), light intensity (Zhu and Thomas, 1995; but see Kakizawa et al, 1995), fasting (Company et al, 2001; but see Kakizawa et al, 1995), etc. Although SL may (or may not; as exemplified by the above conflicting evidence between species) have important functions during these circumstances, its direct effects on the body remain unknown from these observations.…”

Section: Introductionmentioning

confidence: 97%

Effects of constitutive expression of somatolactin alpha on skin pigmentation in medaka

Fukamachi

Yada

Meyer

et al. 2009

Gene

View full text Add to dashboard Cite

Somatolactin alpha (SLa) is a fish-specific peptide hormone secreted from the pituitary. Its functions have been investigated for the last decade but are still under debate. We previously reported a frame-shift mutation on SLa in a medaka mutant, color interfere (ci), which shows defects in body-color regulation, lipid storage, and cortisol secretion. In this study, we examined the effects of introducing a DNA fragment which constitutively expresses wild-type SLa under regulation of the beta-actin (Actb) promoter into the ci genome. We successfully visualized the transgene expression by taking advantage of an internal ribosome entry site (IRES) and the green fluorescent protein (GFP). The transgenic medaka, Actb-SLa:GFP, exhibited a reversed body-color phenotype of ci; more orange xanthophores and less white leucophores. We also detected more black melanophores and less silver iridophores, which indicates SLa's comprehensive role in regulating all types of pigment cells in the skin. Unexpectedly, the defects in lipid/cortisol contents remained in Actb-SLa:GFP. Therefore, the causal relationship between SLa and lipid/cortisol metabolisms relapses to an open question which needs to be reassessed by other types of experiments. Both the Actb-SLa:GFP and ci fish grow and mature similar to wild type, indicating SLa contributes little to growth regulation in spite of the fact that it binds to a teleost-specific paralog of growth-hormone receptor in vitro. The present study provides definitive evidence for SLa's principal and indispensable role in body-color regulation in medaka.

show abstract

Section: Introductionmentioning

confidence: 97%

Effects of constitutive expression of somatolactin alpha on skin pigmentation in medaka

Fukamachi

Yada

Meyer

et al. 2009

Gene

View full text Add to dashboard Cite

show abstract

“…The habitat of the carp (Cyprinus carpio) undergoes seasonal changes that compel the fish to develop adaptive strategies for sensing the cyclical fluctuation of the physical parameters (e.g. temperature, photoperiod) and to transduce them into molecular signals through mechanisms that involve the neuroendocrine system (Figueroa et al, 1994;Kausel et al, 1998;López et al, 2001). One of the most outstanding cellular features of this adaptive process is the reorganization of the nucleolus architecture.…”

Section: Introductionmentioning

confidence: 99%

Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio

Vera¹,

Molina²,

Pinto

et al. 2003

Biol. Res.

Self Cite

View full text Add to dashboard Cite

The seasonal adaptation of the teleost Cyprinus carpio to the cyclical changes of its habitat demands physiological compensatory responses. The process involves profound nucleolar adjustments and remarkable changes in rRNA synthesis, which affects ribosomal biosynthesis. In this context, we have demonstrated that the synthesis of several proteins involved in ribosomal biogenesis as protein kinase CK2, ribosomal protein L41 and nucleolin, as well as U3 snoRNP, are differentially regulated in summer-acclimatized carp compared to the cold-season adapted fish. To understand the mechanisms involved in the seasonal regulation of rRNA gene transcription, we have been studying the carp rDNA cistron structure. Because the cis-elements that regulate the expression of the tandem organized ribosomal genes are located in the non-transcribed intergenic spacer (IGS), we analyzed the primary structure of the carp rDNA gene IGS. The gene organization is similar to that described from other vertebrate species, including numerous repetitive sequences, the transcription start site, and some potential cis-elements such as ribosomal enhancers, proximal terminator and transcriptional terminators. Ribosomal DNA is a remarkable case of gene duplication and has been used as a model to test the concerted evolution theory. We performed sequence comparison analyses of 18S rRNA coding sequences from carp with different species, data with which an unrooted phylogram was constructed.

show abstract

“…Furthermore, Pit-1 exhibits a concurrent cell and temporal transcription with the hormone [Kausel et al, 1999]. While GH also is induced environmentally in the carp [Figueroa et al, 2005], the expression of somatolactin, a fish hormone belonging to the PRL/GH family, seems not to be associated with the seasonal acclimatization process [Lopez et al, 2001].…”

mentioning

confidence: 99%

Gene structure and seasonal expression of carp fish prolactin short receptor isoforms

Martin¹,

Hurtado²,

Quezada³

et al. 2006

J of Cellular Biochemistry

Self Cite

View full text Add to dashboard Cite

The complex adaptive mechanisms that eurythermal fish have evolved in response to the seasonal changes of the environment include the transduction of the physical parameter variations into neuroendocrine signals. Studies in carp (Cyprinus carpio) have indicated that prolactin (PRL) and growth hormone (GH) expression is associated with acclimatization, suggesting that the pituitary gland is a relevant physiological node in this adaptive process. Also, the distinctive pattern of expression that carp prolactin receptor (PRLr) protein depicts upon seasonal acclimatization supports the hypothesis that PRL and its receptor clearly are involved in the new homeostatic stage that the eurythermal fish needs to survive during the cyclical changes of its habitat. Here, we characterize the first prolactin receptor gene in a teleost and show that its expression is not associated with alternative promoters, unlike in humans and rodents. Using the regulatory region to direct the transcription of green fluorescent protein (GFP) in zebrafish embryos, we mapped the appearance of this hormone receptor during fish development. This is the first report identifying a fish prolactin receptor gene expressing transcript isoforms encoding for short forms of the protein (45 kDa). These have been found in osmoregulatory tissues of the carp and are regulated in connection with the seasonal acclimatization of the fish.

show abstract

In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio)

Cited by 10 publications

References 0 publications

Effects of constitutive expression of somatolactin alpha on skin pigmentation in medaka

Effects of constitutive expression of somatolactin alpha on skin pigmentation in medaka

Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio

Gene structure and seasonal expression of carp fish prolactin short receptor isoforms

Contact Info

Product

Resources

About