In situ hybridization with genomic and rDNA probes reveals complex origins for polyploid New Zealand species of<i>Plantago</i>(Plantaginaceae)

Wong, C; Murray, B. G.

doi:10.1080/0028825x.2014.898664

Cited by 5 publications

(4 citation statements)

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“…All individuals from the four Hungarian populations were diploid (2 n = 2x = 12) as Soó ( 1970 ) reported previously. The characterization and physical mapping of rDNA sites in Plantago resulted in species-specific patterns (Dhar et al 2006 , 2017 ; Wong and Murray 2014 ). Each Plantago species investigated so far has unique rDNA pattern enabling discrimination of species according to the rDNA loci position.…”

Section: Discussionmentioning

confidence: 99%

“…is 4, 5 and 6 (Peruzzi and Cesca 2002 ; Dhar et al 2006 ; Shahriari et al 2018 ). The majority of Plantago species are diploids, however, tetraploids, hexaploids, octoploids, decaploids, dodecaploids, and 16-ploids are also reported (Dhar et al 2006 ; Wong and Murray 2014 ). Previously, it was reported that P. maxima is tetraploid 2 n = 4x = 24 (Magulaev 1982 ).…”

Section: Introductionmentioning

confidence: 99%

“…Mapping of rDNA loci using fluorescence in situ hybridization can be used to determine phylogenetic relationships in the Plantago genus (Dhar et al 2006 ). Until this study the chromosomal position of 35S and 5S rDNA loci has been investigated for fewer than 10 Plantago species, exhibiting from one to two 5S rDNA loci and from one to two 35S rDNA loci (Dhar et al 2006 , 2017 ; Wong and Murray 2014 ). rDNA loci number and position is so far unknown for P. maxima .…”

Section: Introductionmentioning

confidence: 99%

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Living on the edge: morphological, karyological and genetic diversity studies of the Hungarian Plantago maxima populations and established ex situ collection

2023

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Background The analysis of genetic diversity of protected plant species can greatly support conservation efforts. Plantago maxima Juss. ex Jacq. is a perennial species distributed along the Eurasian steppe. The westernmost range edge of the species’ distribution is located in the Pannonian basin, in Hungary where it is represented by a few, fragmented and highly endangered populations. We studied population diversity of all Hungarian range edge, natural populations, and one established ex situ population. One population from the centre of distribution (Kazakhstan) was implemented in the cpDNA haplotype study to compare the peripheral vs. central populations. We performed morphometric trait-based analysis, chromosome studies (morphometric analyses and FISH) and genetic diversity evaluations using inter simple sequence repeats (ISSR) and cpDNA trnL-trnF to evaluate differences between the in situ and ex situ populations as well as central vs. peripheral populations. Results Our results showed no obvious morphological differences among the in situ and ex situ populations in the period between 2018 and 2020. One ex situ subpopulation develops flowers three years in a row from 2019, which is a favourable indicator of the introduction success. Hungarian populations are exclusively diploids (2n = 2x = 12). The karyogram consists of 5 metacentric and 1 acrocentric chromosome pair. Plantago maxima has one 35S and two 5S rDNA loci, located on the acrocentric chromosome pair. Eight variable ISSR primers yielded 100 fragments, of which 74.6% were polymorphic (mean He = 0.220). A high level of genetic variation within population was observed (92%) while the genetic differentiation among the populations was only 8%. STRUCTURE analysis revealed that the largest Kunpeszér population separated from the rest of the Hungarian populations, indicating a high rate of admixture among the other ones. Based on the trnL-trnF sequence analysis the Hungarian populations represent a single haplotype, which can indicate a reduced diversity due to isolation and recent population decline. By contrast, Kazakh population represents a distinct haplotype compared to the Hungarian samples. Conclusions The present study draws the attention to the high conservation value of the Plantago maxima populations from the westernmost range edge of the species’ distribution.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Living on the edge: morphological, karyological and genetic diversity studies of the Hungarian Plantago maxima populations and established ex situ collection

2023

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“…GISH has an ability to distinguish genomic relationship of polyploids. In polyploids, GISH effectively confirmed the presumed parental genomes and in addition this technique also provides their origin information, that is, whether polyploids are alloploids or autoploids ( Table 1 ) [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Genomic In Situ Hybridization in Horticultural Science

Ramzan

Younis

Lim

2017

International Journal of Genomics

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Molecular cytogenetic techniques, such as in situ hybridization methods, are admirable tools to analyze the genomic structure and function, chromosome constituents, recombination patterns, alien gene introgression, genome evolution, aneuploidy, and polyploidy and also genome constitution visualization and chromosome discrimination from different genomes in allopolyploids of various horticultural crops. Using GISH advancement as multicolor detection is a significant approach to analyze the small and numerous chromosomes in fruit species, for example, Diospyros hybrids. This analytical technique has proved to be the most exact and effective way for hybrid status confirmation and helps remarkably to distinguish donor parental genomes in hybrids such as Clivia, Rhododendron, and Lycoris ornamental hybrids. The genome characterization facilitates in hybrid selection having potential desirable characteristics during the early hybridization breeding, as this technique expedites to detect introgressed sequence chromosomes. This review study epitomizes applications and advancements of genomic in situ hybridization (GISH) techniques in horticultural plants.

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The application of high-throughput sequencing for taxonomy: The case of Plantago subg. Plantago (Plantaginaceae)

Hassemer

Bruun-Lund

Shipunov

et al. 2019

Molecular Phylogenetics and Evolution

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In situ hybridization with genomic and rDNA probes reveals complex origins for polyploid New Zealand species ofPlantago(Plantaginaceae)

Cited by 5 publications

References 42 publications

Living on the edge: morphological, karyological and genetic diversity studies of the Hungarian Plantago maxima populations and established ex situ collection

Living on the edge: morphological, karyological and genetic diversity studies of the Hungarian Plantago maxima populations and established ex situ collection

Application of Genomic In Situ Hybridization in Horticultural Science

The application of high-throughput sequencing for taxonomy: The case of Plantago subg. Plantago (Plantaginaceae)

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