In situ Identification of Ammonia-oxidizing Bacteria

Wagner, Michael; Rath, Gabriele; Amann, Rudolf; Koops, Hans‐Peter; Schleifer, Karl‐Heinz

doi:10.1016/s0723-2020(11)80396-6

Cited by 482 publications

(300 citation statements)

References 50 publications

Supporting

Mentioning

277

Contrasting

Unclassified

Order By: Relevance

“…The specific oligonucleotide probes used in this study are: EUB mix for the detection of the domain bacteria (Amann et al 1990), NSO190 for the detection of betaproteobacterial ammonia-oxidizing bacteria (AOB) (Mobarry et al 1996), NEU for most of the halophilic and halotolerant Nitrosomonas sp. (Wagner et al 1995), NSMR76 for Nitrosomonas marina-like AOB (Burrell et al 2001), NIT3 for Nitrobacter sp. (Wagner et al 1995), and NTSPA685 for the Nitrospira moscoviensis, Nitrospira marina, aquarium clone 710-9 (Hovanec et al 1998).…”

Section: Fish Dna Extraction and Real-time Quantitative Pcr Analysismentioning

confidence: 99%

Start-up of halophilic nitrogen removal via nitrite from hypersaline wastewater by estuarine sediments in sequencing batch reactor

Cui

Ding

et al. 2013

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Nitrogen removal from hypersaline wastewater was successfully started up by inoculating estuarine sediments for 140 days. Efficient ammonia and total nitrogen removal was sustained under specific ammonia loading of 0.016-0.139 kg N/[kg VSS day] in a sequencing batch reactor. Stable nitrite accumulation was observed during nitrification. The specific ammonia consumption rate was higher than the value of freshwater activated sludge and saltacclimated freshwater activated sludge. With methanol as carbon source, specific nitrite reduction rate of halophilic denitrifiers was much less than the freshwater counterpart. Halophilic activated sludge was characterized as good settling and flocculation prosperity with small floc size and netlike sludge structure. The abundance of ammonia-oxidizing bacteria outnumbered ammonia-oxidizing archaeas in both estuarine sediments and the activated sludge. Nitrifier population was dominated by the halophilic members of genus Nitrosomonas. This study demonstrated the application of mixed halophilic consortia for efficient nitrogen removal, overcoming the limits and difficulties of applying freshwater bacteria for saline wastewater treatment.

show abstract

Section: Fish Dna Extraction and Real-time Quantitative Pcr Analysismentioning

confidence: 99%

Start-up of halophilic nitrogen removal via nitrite from hypersaline wastewater by estuarine sediments in sequencing batch reactor

Cui

Ding

et al. 2013

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…The ammonia-oxidizing and nitrite-oxidizing bacteria were identified using previously published probes ( Fig. 3; [10][11][12][13][14]). …”

Section: Fluorescence In Situ Hybridization Microscopy and Quantificmentioning

confidence: 99%

Nitrifying and heterotrophic population dynamics in biofilm reactors: effects of hydraulic retention time and the presence of organic carbon

Melo²,

et al. 2002

Self Cite

View full text Add to dashboard Cite

Two biofilm reactors operated with hydraulic retention times of 0.8 and 5.0 h were used to study the links between population dynamics and reactor operation performance during a shift in process operation from pure nitrification to combined nitrification and organic carbon removal. The ammonium and the organic carbon loads were identical for both reactors. The composition and dynamics of the microbial consortia were quantified by fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes combined with confocal laser scanning microscopy, and digital image analysis. In contrast to past research, after addition of acetate as organic carbon nitrification performance decreased more drastically in the reactor with longer hydraulic retention time. FISH analysis showed that this effect was caused by the unexpected formation of a heterotrophic microorganism layer on top of the nitrifying biofilm that limited nitrifiers oxygen supply. Our results demonstrate that extension of the hydraulic retention time might be insufficient to improve combined nitrification and organic carbon removal in biofilm reactors. r

show abstract

“…This might be due to the choice of the probes. Although probe Nso 190 detected many ammonia oxidizers of the b-subclass of Proteobacteria [35], some Nitrosomonas strains were not included [40], and probe NEU is only specific for the detection of a few members of the genus Nitrosomonas [40,50]. Therefore it is not remarkable that strain R1.24, which was classified by IF labeling to genus Nitrosomonas, could not be detected by probes Nso 190 and NEU.…”

Section: Discussionmentioning

confidence: 99%

“…Probe EUB338 was used to detect members of the domain Bacteria [12], and probe Nso 190 detects all ammonia oxidizers of the b-subclass of Proteobacteria [35] except some Nitrosomonas strains (Table 1, [40]). Probe NEU is specific for the detection of only a few members of the genus Nitrosomonas (Table 1 [40,50]). Probe Nsv 443 is directed to Nitrosolobus multiformis, Nitrosospira briensis and Nitrosovibrio tenuis (Table 1 [35,40]).…”

Section: Fishmentioning

confidence: 99%

Genera-Specific Immunofluorescence Labeling of Ammonia Oxidizers with Polyclonal Antibodies Recognizing Both Subunits of the Ammonia Monooxygenase

Fiencke

Bock

2004

Microb Ecol

View full text Add to dashboard Cite

Polyclonal antibodies that recognize the two subunits AmoA and AmoB of the ammonia monooxygenase (AMO) were applied to identify ammonia-oxidizing bacteria by immunofluorescence (IF) labeling in pure, mixed, and enriched cultures. The antibodies against the AmoA were produced using a synthetic peptide of the AmoA of Nitrosomonas eutropha, whereas the antibodies against the AmoB had been developed previously is against the whole B-subunit of the AMO [Pinck et al. (2001) Appl Environ Microbiol 67:118-124]. Using IF labeling, the AmoA antibodies were specific for the detection of all species of the genus Nitrosomonas. In contrast, the antiserum against AmoB labeled all genera of ammonia oxidizers of the b-subclass of Proteobacteria (Nitrosomonas, Nitrosospira, Nitrosolobus, and Nitrosovibrio). The fluorescence signals of the AmoA antibodies were spread all over the cells, whereas the signals of the AmoB antibodies were associated with the cytoplasmic membranes. The specificity of the reactions of the antisera with ammonia oxidizers were proven in pure and mixed cultures, and the characteristic IF labeling and the morphology of the cells enabled their identification at the genus level. The genus-specific IF labeling could be used to identify ammonia oxidizers enriched from various habitats. In enrichment cultures of natural sandstone, cells of the genera Nitrosomonas, Nitrosovibrio, and Nitrosospira were detected. Members of the genus Nitrosovibrio and Nitrosolobus were most prominent in enriched garden soil samples, whereas members of the genus Nitrosomonas dominated in enriched activated sludge. The antibodies caused only slight background fluorescence on sandstone and soil particles compared to oligonucleotide probes, which could not be used to detect ammonia oxidizers on these materials because of strong nonspecific fluorescence.

show abstract

In situ Identification of Ammonia-oxidizing Bacteria

Cited by 482 publications

References 50 publications

Start-up of halophilic nitrogen removal via nitrite from hypersaline wastewater by estuarine sediments in sequencing batch reactor

Start-up of halophilic nitrogen removal via nitrite from hypersaline wastewater by estuarine sediments in sequencing batch reactor

Nitrifying and heterotrophic population dynamics in biofilm reactors: effects of hydraulic retention time and the presence of organic carbon

Genera-Specific Immunofluorescence Labeling of Ammonia Oxidizers with Polyclonal Antibodies Recognizing Both Subunits of the Ammonia Monooxygenase

Contact Info

Product

Resources

About